JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2016, Vol. 40 ›› Issue (02): 81-87.doi: 10.3969/j.issn.1000-2006.2016.02.014

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Influence of leaf litter and soil leaching liquor on seed germination and seedling growth of Pinus koraiensis

CHEN Lixin1, LI Shaobo1, QIAO Lu2, BU Fan1, DUAN Wenbiao1*   

  1. 1.College of Forestry, Northeast Forestry University,Harbin 150040, China;
    2.Henan University of Chinese Medicine, Zhengzhou 450008, China
  • Online:2016-04-18 Published:2016-04-18

Abstract: The current fallen leaves from Pinus koraiensis, Tilia amurensis and Betula costata, and their fresh leaves, 0-10 cm soil samples were collected from the P. koraiensis plantation, T. amurensis-P. koraiensis and B. costata-P. koraiensis mixed forests in Liangshui National Nature Reserve in Heilongjiang Province. After the leaves and soil samplings were air dried, smashed(ground), sieved, soaked and filtered, ten types of solutions, including five treatments of leaching liquors with 4 different concentration gradients(1:10, 1:30, 1:50, 1:100)and sterilized distilled water(CK), were equivalently put into culture dishes containing pregerminated, sterilized, and washed P. koraiensis seeds and experimental pots with P. koraiensis seedlings. The germination test and pot experiment were performed respectively to measure the 7 indices including germination percentage, germination potential, hypocotyl and radicle length, seedling height, root length and dry mass. The results showed that the different concentration leaching liquors inhibited the germination of P. koraiensis seeds for all forest types except 1:100 concentration for T. amurensis-P. koraiensis and B. costata-P. koraiensis mixed forests, their mean germination percentage successively ranked in the CK(47%)>T. amurensis-P. koraiensis mixed forest(44%)>B. costata-P. koraiensis mixed forest(42%)>P. koraiensis plantation(33%). High concentration(1:10)leaching liquor had suppressive effects on the 7 measured indices mentioned above, and the strongest suppressive effect was caused by the soil leaching liquor in P. koraiensis plantation, they significantly decreased by 55.32%, 51.35%, 54.07%, 55.51%, 15.93%, 58.01%, 35.71%(P<0.05)as compared with the CK, respectively. The suppressive effects of leaching liquor became weak with its decreasing concentration, it would disappear at a certain concentration or turned into promoting effects. The strongest promoting effects on the other 6 indices except for the seedling height resulted from low concentration(1:100)leaching liquor of T. amurensis fallen leaves, they significantly increased by 38.30%, 37.84%, 31.10%, 25.94%, 61.34%, 21.43%(P<0.05)in comparison with the CK, respectively. The strongest promoting effects on the seedling height came from low concentration(1:100)leaching liquor of T. amurensis fresh leaves, it was significantly raised by 15.13%(P<0.05)compared by the CK.

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