Effects of different carbon sources on somatic embryo of Cinnamomum camphora L.

doi:10.3969/j.issn.1000-2006.201904002

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2020, Vol. 44 ›› Issue (1): 54-62.doi: 10.3969/j.issn.1000-2006.201904002

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Effects of different carbon sources on somatic embryo of Cinnamomum camphora L.

JING Ruyue¹(), WANG Peilan¹, HUANG Zhen², HE Linjun¹, LI Zhihui¹^,^*()

1. College of Forestry, Central South Forestry University of Science and Technology, Changsha 410004, China
2. Sichuan Academy of Forestry Sciences,Chengdu 610081, China

Received:2019-04-01 Revised:2019-06-20 Online:2020-02-08 Published:2020-02-02
Contact: LI Zhihui E-mail:326040597@qq.com;lzh1957@126.com

Abstract

Abstract:

【Objective】 In this study, we investigated the effects of somotic adjustment substances on somatic embryogenesis and regeneration of Cinnamomum camphora L. plants, which will provide a foundation for enhancing the induction rate of somatic embryogenesis and optimizing a somatic embryogenesis system for this species. 【Method】 The immature zygotic embryos of C. camphora collected in early July in Chenzhou, Changsha, Loudi and Huaihua City were used as materials for examining the effects of different carbon source types, concentrations, and combinations on somatic embryogenesis. The somatic embryo induction rate, germination rate, and rooting rate of plants were determined under different conditions.【Result】 The suitable medium for somatic embryo induction was MS medium+1.0 mg/L 6-benzylaminopurine (6+BA)+0.3 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D)+1.0 mg/L streptomycin+15 g/L sucrose+30 g/L sorbitol+6.5 g/L agar+700 mg/L hydrolyzed casein; a suitable proliferation medium was MS+15 g/L sourse +30 g/L sorbitol+6.5 g/L agar+700 mg/L hydrolyzed casein; a suitable medium for bud germination was murashige and skoog medium+0.1 mg/L thidiazuron (TDZ)+0.5 mg/L naphthylacetic acid (NAA)+0.1 mg/L 3-indolebutyric acid (IBA)+15 g/L sucrose+15 g/L sorbitol+6.5 g/L agar; and a suitable medium for rooting was MS+1.0 mg/L IBA+15 g/L sucrose+15 g/L sorbitol+6.5 g/L agar. 【Conclusion】 As a consequence of modification, somatic embryo induction was significantly improved by adding 15 g/L sucrose and 30 g/L sorbitol as combined carbon sources, and the new plants thus generated had a larger number of rooting strips, obvious main roots, and better growth characteristics.

Key words: Cinnamomum camphora L., carbon sources, somatic embryo induction rate, bud germination rate, rooting rate

CLC Number:

S792.23

JING Ruyue, WANG Peilan, HUANG Zhen, HE Linjun, LI Zhihui. Effects of different carbon sources on somatic embryo of Cinnamomum camphora L.[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2020, 44(1): 54-62.

Figures/Tables 9

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碳源种类 types of carbon sources	体胚诱导率/% induction rate of somatic embryo
碳源种类 types of carbon sources	郴州 Chenzhou	长沙 Changsha	怀化 Huaihua	娄底 Loudi
蔗糖sucrose	72.9±2.6 aC	83.7±1.1 aA	77.4±1.5 aB	69.1±1.3 aD
葡萄糖glucose	53.2±2.7 bB	59.7±1.9 bA	45.2±2.1 bC	44.9±1.6 bC
麦芽糖maltose	0.0	10.3±3.9 eA	3.5±1.7 dB	0.0
山梨醇sorbitol	33.3±1.5 cC	45.3±1.3 cA	46.4±1.9 bA	37.9±1.1 cB
甘露醇mannitol	31.9±2.4 cB	44.7±1.5 cA	44.5±2.3 bA	32.5±3.2 dB
海藻糖trehalose	11.2±1.6 dB	22.3±2.6 dA	18.7±3.9 cA	19.7±1.3 eA

因素类型 factor type	factor 系数 coefficient	标准差 standard deviation	标准化系数 standard coefficient	t	P
常量constant	67.786	7.339		9.236	<0.001^***
产地different original location	-0.425	2.055	-0.020	-0.207	0.837
碳源种类types of carbon sources	-8.251	1.346	-0.594	-6.132	<0.001^***
整体模型integral model	R²=0.353 F (2,69) =18.82		R²=0.334 P<0.001^***

混合碳源质量浓度/(g·L^-1) concentration of two mixed carbon sources			体胚诱导率/% induction rate of somatic embryo
蔗糖 source	山梨醇 sorbitol	甘露醇 mannitol	体胚诱导率/% induction rate of somatic embryo
45(CK)			84.1±1.3 c
15	15		87.7±2.3 b
15	30		92.9±1.7 a
15	45		55.3±1.4 d
15	60		31.9±2.5 e
45(CK)			84.1±1.3 b
15		15	85.4±1.7 b
15		30	90.8±1.1 a
15		45	55.1±3.2 c
15		60	30.7±2.5 d

碳源种类 types of carbon sources	体胚芽萌发率/% bud germination rate	体胚生根率/% rooting rate
蔗糖source	46.3±1.4 a	83.7±1.4 a
葡萄糖glucose	35.4±1.9 b	81.3±3.6 a
麦芽糖maltose	0.0	0.0
山梨醇sorbitol	45.3±1.3 a	83.3±1.6 a
甘露醇mannitol	44.4±1.9 a	82.0±2.9 a
海藻糖trehalose	19.4±3.2 c	40.3±1.2 b

蔗糖质量浓度 / (g·L^-1) concentration of sucrose	体胚芽萌发率/% bud germination rate	体胚生根率/% rooting rate
10	41.2±5.7 b	63.3±2.1 b
20	53.3±1.2 a	85.4±4.2 a
30	46.3±1.4 b	83.7±1.4 a

Effects of different carbon sources on somatic embryo of Cinnamomum camphora L.

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两种混合碳源质量浓度/(g·L^-1) concentration of two mixed carbon sources			体胚芽萌发率/% bud germination rate	体胚生根率/% rooting rate
蔗糖 sucrose	山梨醇 sorbitol	甘露醇 mannitol	体胚芽萌发率/% bud germination rate	体胚生根率/% rooting rate
45(CK)			53.3±1.2 a	85.4±4.2 a
15	15		54.7±1.7 a	86.9±3.7 a
15	30		47.3±1.7 b	84.3±3.5 a
15	45		47.2±3.6 b	84.2±3.2 a
45(CK)			53.3±1.2 a	85.4±4.2 a
15		15	53.6±2.7 a	86.0±2.3 a
15		30	46.8±1.6 b	83.9±1.6 a
15		45	41.9±2.6 c	64.4±2.3 b

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