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Cloning and expression analysis of the CiDGAT1 from Carya illinoinensis
ZHOU Xiaofeng, MO Zhenghai, SHANG Yangjuan, LUO Min, SU Wenchuan, TAN Pengpeng, HAN Minghui, DENG Qiuju, TIAN Zhaoxia, PENG Fangren
JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2020, Vol. 44 ›› Issue (4) : 55-62.
PDF(8431 KB)
PDF(8431 KB)
Cloning and expression analysis of the CiDGAT1 from Carya illinoinensis
The purpose of the study was to elucidate the structure and expression patterns of the CiDGAT1 to provide a theoretical reference for a further analysis of the function of CiDGAT1 gene.
A full-length CiDGAT1 gene was isolated from the fruit of Carya illinoinensis using rapid amplification of cDNA ends with specific primers according to the conserved DGAT1 fragment sequence. The genetic structure , protein character, and evolutionary relationship were analyzed using bioinformatics. In addition, the CiDGAT1 temporal expression pattern was analyzed in different varieties using quantitative real-time PCR.
CiDGAT1 has an open reading frame with 1 617 bp and encodes 538 amino acids. The gene structure analysis showed that the CiDGAT1 gene consists of 15 exons and 14 introns. The phylogenetic analysis revealed that the CiDGAT1 protein is clustered in the dicotyledonous branch with a close relationship to oak DGAT1 and filbert DGAT1. The qRT-PCR analysis showed that the expression of CiDGAT1 is higher in the growth process of ‘Pawnee’ and peaks in the mature stage at 145 d. Whereas, CiDGAT1 expression is lower during the growth process of ‘Mahan’ and ‘Jinhua’ varieties; it rapidly increased after 152 d, reaching the highest level at final maturity.
CiDGAT1 belongs to the MBOAT gene family, and its expression tends to increase during the growth process of the fruit of C. illinoinensis, which is a key regulator in the process of oil synthesis in plants.
Carya illinoinensis / lipids synthesis / CiDGAT1 / gene cloning / gene expression patterns
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