JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2004, Vol. 28 ›› Issue (03): 86-88.doi: 10.3969/j.jssn.1000-2006.2004.03.022

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Construction and Analysis of cDNA Library from Leaf of Lycoris aurea

CUI Yong-lan,HUANG Min-ren~*,WANG Ming-xiutry University,Nanjing 210037,China)   

  • Online:2004-06-18 Published:2004-06-18

Abstract: Lycoris aurea is a perennial,bulbiferous plant.Its vegetative growth and reproduction are discrete.To study the leaf development of Lycoris aurea,A cDNA library was constructed from Lycoris aurea leaves.The titer of primary library is 5.6×10~5 pfu/mL,and the titer of amplified library is 6.9×10~9 pfu/mL,in which 96% of the phages were recombinant.Insert sizes ranging from 500 bp to 2 500 bp were obtained.All of the above mentioned accorded with the general requirements of cDNA library construction,which established a good foundation for investigating the leaf development and cloning full-length cDNA of genes related to leaf development of Lycoris aurea with the methodology of both genomics and molecular biology.

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