Abstract: The dynamic changes of endogenous hormone contents in Betula luminifera were mesured by ELISA during adventitious buds and roots formation, which were cultured on the modified MS medium of plus with 4.5 mg/L BA and 0.01 mg/L NAA and on modified WH medium plus with 3.0 mg/L BA and 1.5 mg/L NAA respectively. It was showed that the significant effect between endogenous hormone and exogenous hormone in vitro culture during morphogenesis. The results provided the properly combination for setting in vitro culture system. It was also proofed that exogenous hormone endogenous hormone to regulate the development of adventitious buds and roots during morphogenesis. With the increasing endogenous hormone, including ZR, ABA, IAA, promoting the development of adventitious buds, and with the increasing endogenous hormone, including IAA, ABA, iPA, promoting the formation and development of root system.