Chromosomal Giemsa Cbanding analysis for root tips of Lilium speciosum Thunb.var.glorosoides Baker

doi:10.3969/j.jssn.1000-2006.2009.03.005

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2009, Vol. 33 ›› Issue (03): 17-19.doi: 10.3969/j.jssn.1000-2006.2009.03.005

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Chromosomal Giemsa Cbanding analysis for root tips of Lilium speciosum Thunb.var.glorosoides Baker

HU Fengrong, LIU Guangxin, XI Mengli, WU Zhuhua, SHI Jisen*

Key Laboratory of Forest Genetics and Biotechnology of Ministry of Education, Nanjing Forestry University, Nanjing 210037, China

Online:2009-06-18 Published:2009-06-18

Abstract

Abstract: Chromosomes from root tips of Lilium speciosum Thunb.var.glorosoides Baker were analyzed by Giemsa Cbanding. The results shown that, the karyotype is 2n=24=8C+4CI++2CI++2CN+4I++2I+T++2, the banding number of haploid genome was 20. Strong banding were observed on the centromeric place of chromosome A, E, H, J, K, and short arm of chromosome F. Chromosome A is SATchromosome, and strong banding were observed on the secondary constriction. Three different intensity bandings were shown on the short arm of chromosome B. Each chromosome of Lilium speciosum Thunb.var.glorosoides Baker could be distinguished by Giemsa Cbanding. The Cbanding results derived from this study provided reliable evidence for the identification of hybrid and the chromosomal location of the interesting genes.

CLC Number:

S722

HU Fengrong, LIU Guangxin, XI Mengli, WU Zhuhua, SHI Jisen*. Chromosomal Giemsa Cbanding analysis for root tips of Lilium speciosum Thunb.var.glorosoides Baker[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2009, 33(03): 17-19.

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Chromosomal Giemsa Cbanding analysis for root tips of Lilium speciosum Thunb.var.glorosoides Baker

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