Comparison of RAPD fingerprints of fruiting mei generated by different electrophoresis and characterization of the amplified sequences

doi:10.3969/j.jssn.1000-2006.2010.05.006

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2010, Vol. 34 ›› Issue (05): 29-33.doi: 10.3969/j.jssn.1000-2006.2010.05.006

Previous Articles Next Articles

Comparison of RAPD fingerprints of fruiting mei generated by different electrophoresis and characterization of the amplified sequences

LI Xiaoying, SONG Changnian, ZHANG Yanping, WANG Yujuan, ZHANG Zhen, FANG Jinggui^*

College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China

Online:2010-10-08 Published:2010-10-08

Abstract

Abstract: PCR amplification was conducted with 11 bp primers by the optimized RAPD protocol and we used agarose and polyacrylamide gel (PAGE) electrophoresis to detect RAPD amplification products from 16 fruiting mei cultivars. The results showed that the total and polymorphic bands of PAGE were 204 and 145, while 91 and 58 on agarose gel. The polygenetic tree also showed that all the 16 cultivars could be distinguished unambiguously and their relationship was revealed according to the fingerprinting profiles obtained by PAGE electrophoresis system. Random cloning and sequencing of 20 amplified fragments showed that all these segments were RAPD products generated by the corresponding primers. Among the sequenced fragments, four sequences were from protein coding regions, suggesting RAPD could randomly amplify both coding and noncoding sequences. Thus, genetic variability of different fruiting mei cultivars could be properly reflected by RAPD marker.

CLC Number:

S722

LI Xiaoying, SONG Changnian, ZHANG Yanping, WANG Yujuan, ZHANG Zhen, FANG Jinggui. Comparison of RAPD fingerprints of fruiting mei generated by different electrophoresis and characterization of the amplified sequences[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2010, 34(05): 29-33.

References

[1]Williams J G K, Kubelik A R, Livak K J, et al. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers[J]. Nucl Acids Res, 1990, 18(22): 6531-6535. [2]Lin K H, Lai Y C, Li H C. Genetic variation and its relationship to root weight in the sweet potato as revealed by RAPD analysis[J]. Scientia Horticulturae, 2009, 120(1): 2-7. [3]An N, Guo H B, Ke W D. Genetic variation in Rhizome Lotus (Nelumbo nucifera Gaertn.ssp.nucifera) germplasms from China assessed by RAPD markers[J]. Agricultural Sciences in China, 2009, 8(1): 31-39. [4]Yang R W, Zhou Y H, Ding C B, et al. Relationships among Leymus species assessed by RAPD markers[J]. Biologia Plantarum, 2008, 52(2): 237-241. [5]Ozakit, Shimada T, Nakanishi T, et al. RAPD analysis for parentage determination in Prunus mume Sieb.Et Zucc.[J]. Japan Soc Hort Sci, 1995, 64(2): 235-242. [6]Shimada T H T, Yamaguchi T. Classification of mume (Prunus mume Sieb.et Zucc.) by RAPD assay[J]. J Japan Soc Hort Sci, 1994, 63(3): 543-551. [7]房经贵,章镇,蔡斌华,等. 果梅品种分类研究进展[J]. 江苏林业科技,2009,36(3):44-50. [8]房经贵,章镇,周兰华. RAPD标记鉴定果树芽变的可行性分析[J]. 果树学报,2001,18(3):182-185. [9]于华平,房经贵,张美勇,等. RAPD标记用于葡萄、苹果等7种果树的品种鉴定的研究[J]. 江西农业学报,2009,21(10):5-9. [10]房经贵,王晨,章镇,等. 浅谈中国果树品种权益保护及其现状[J]. 中国农学通报,2009,25(24):298-302. [11]佟兆国,王富荣,章镇,等. 一种从果树成熟叶片提取DNA的方法[J]. 果树学报2008,25(1):122-125. [12]刘勇,吴波,孙中海,等. 江西柑橘地方品种资源及野生近缘种SSR分子标记[J]. 江西农业大学学报,2005,27(4):486-490. [13]程中平,陈志伟,胡春根,等. 利用RAPD技术对新疆桃分类地位的探讨[J]. 园艺学报,2001,28(3):211-217. [14]程中平,陈志伟,胡春根,等. 油桃品种的RAPD分析[J]. 中国农业大学学报,2002,7(3):63-68. [15]乔玉山,章镇,房经贵,等. 中国李RAPD的优化反应体系及其在品种鉴定中的应用[J]. 果树学报,2003,20(6):445-449. [16]吴少华,张大生,潘东明,等. 应用RAPD技术对柰、李、桃亲缘关系的探讨[J]. 园艺学报,2002,29(1):66-68. [17]张俊卫,包满珠,陈龙清,等. 桃、梅、李、杏、樱的RAPD分析[J]. 北京林业大学学报,1998,20(2):16-19. [18]高志红,章镇,盛炳成,等. 桃、梅、李、杏四种主要核果类果树RAPD指纹图谱初探[J]. 果树学报,2001,18(2):120-121. [19]陈晓流,陈学森,束怀瑞,等. 15个樱桃品种的RAPD分析[J]. 果树学报,2004,21(6):556-559. [20]程中平,陈志伟,胡春根,等. RAPD技术在桃种质资源保存中的应用[J]. 中国果树,2002(3):5-7. [21]程中平,陈志伟,胡春根,等. 利用RAPD标记对桃品种特殊种质分析[J]. 果树学报,2001,18(4):217-220. [22]乔飞,王力荣,范崇辉,等. 利用RAPD标记评价桃种间杂交一代群体的分离方式[J]. 果树学报,2003,20(4):310-312. [23]冉辛拓,冯志红,于丽辰,等. 利用RAPD方法确认“重阳红”桃及其芽变[J]. 园艺学报,2005,32(4):593.

Related Articles 15

[1]	MA Tan, TIAN Ye, WANG Shujun, LI Wenhao, DUAN Qiying, ZHANG Qingyuan. Sex-specific leaf physiological responses of southern-type poplar to short-term intermittent soil drought [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2024, 48(3): 172-180.
[2]	WANG Gaiping, ZHANG Lei, CAO Fuliang, DING Yanpeng, ZHAO Qun, ZHAO Huiqin, WANG Zheng. Effect of red and blue light quality on growth physiological and flavonoid content of Ginkgo biloba seedlings [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2024, 48(2): 105-112.
[3]	SONG Ziqi, BIAN Guoliang, LIN Feng, HU Fengrong, SHANG Xulan. Establishment and application of a flow cytometry method for chromosome ploidy identification of Cyclocarya paliurus [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2024, 48(2): 61-68.
[4]	SUN Xugao, TAO Jialu, XIE Wei, SHI Jie, ZHANG Baojin, DENG Xiaomei. Optimization of the tissue culture technology system of Mytilaria laosensis trees [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2024, 48(2): 69-78.
[5]	GU Chenrui, YUAN Qihang, JIANG Jing, MU Huaizhi, LIU Guifeng. Mapping leaf shape affecting gene of Betula pendula ‘Dalecarlica’ by association study based on transcriptome sequencing [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2024, 48(1): 39-46.
[6]	YANG Yunli, CAO Li, WANG Yang, GU Chenrui, CHEN Kun, LIU Guifeng. Effects of BpGLK1 interference expression on leaf color and growth of Betula pendula ‘Dalecarlica’ [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2024, 48(1): 18-28.
[7]	WANG Wei, QIU Zhinan, LI Shuang, BAI Xiangdong, LIU Guifeng, JIANG Jing. CRISPR/Cas9 ribonucleoprotein-mediated precise mutation of BpGLK1 in birch without T-DNA insertion [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2024, 48(1): 11-17.
[8]	GUO Ying, YANG Ganggui, WU Yuhan, HE Jie, HE Yujie, LIAO Haoran, XUE Liangjiao. Recent advances in molecular regulatory mechanisms of DNA methylation in plant tissue culture [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2023, 47(6): 1-8.
[9]	CHEN Junna, WANG Xiaoyu, CHEN Chen, PENG Huiwu, CHEN Juan, HUANG Weihe, YU Fangyuan. Effects of BR on enzyme activities related to fatty acid synthesis and oil accumulation of Styrax tonkinensis seeds [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2023, 47(6): 35-41.
[10]	GONG Nan, ZU Xin, XIE Zhijun, ZHU Changhong, LI Shuxian. A low-field nuclear magnetic resonance detection of moisture changes in different water phases during the imbibition and stratification process of Cercis chinensis seeds [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2023, 47(6): 42-50.
[11]	WANG Zhangrong, JI Kongshu, XU Li’an, ZOU Bingzhang, LIN Nengqing, LIN Jingquan. New management model of construction techniques, realistic genetic gain and low cost multi-generation improvement in seedling seed orchard of Pinus massoniana [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2023, 47(6): 9-16.
[12]	OU Yang, OUYANG Fangqun, SUN Meng, WANG Chao, WANG Junhui, AN Sanping, WANG Lifang, XU Na, WANG Meng. Young growth rhythm, annual and density interaction effects and selection strategies of Picea abies clones [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2023, 47(6): 95-104.
[13]	LUO Qianqian, LI Fengqing, XIAO Deqing, DENG Zhangwen, WANG Jianhua, ZHOU Zhichun. Mating system analyses of two natural populations of Taxus wallichiana var. mairei [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2023, 47(5): 80-86.
[14]	GUO Wei, HAN Xiu, ZHANG Li, WANG Ying, DU Hui, YAN Yu, SUN Zhongkui, ZHANG Lin, LI Guohua, LUO Lei. Morphological, photosynthetic physiological and transcriptome analyses of Pteroceltis tatarinowii in response to different nitrogen application levels [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2023, 47(5): 87-96.
[15]	LIU Rong, WU Dejun, WANG Yinhua, REN Fei, LI Li, YAN Liping, ZHOU Xiaofeng. Screening of optimal germination medium for in vitro Fraxinus [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2023, 47(3): 70-76.

Metrics

Comments

www.nldxb.njfu.edu.cn

Edited ＆ Published by Editorial Department of Nanjing Forestry University(Natural Sciences Edition)
Address： No.159 Longpan Road,Nanjing 210037 Jiangsu,P.R.China
E-mail ：xuebao@njfu.edu.cn , xuebao@njfu.com.cn
Telephone +86-25-85428247,85427076
Distributed by China International Book Trading Corporation P.O. Box 399, Beijing ,P.R. China

Comparison of RAPD fingerprints of fruiting mei generated by different electrophoresis and characterization of the amplified sequences

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

Author

Reader

Reviewer