The genetic diversity analysis and molecular ID establishment of Chaenomeles speciosa based on SSR markers

doi:10.12302/j.issn.1000-2006.202301027

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2025, Vol. 49 ›› Issue (1): 59-68.doi: 10.12302/j.issn.1000-2006.202301027

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The genetic diversity analysis and molecular ID establishment of Chaenomeles speciosa based on SSR markers

LI Hui¹^,²(), HOU Lina¹^,², WANG Tianqi², BI Ningning², LI Shengbo³, LIU Zhonghua¹^,²^,^*()

1. National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental, Plants of Ministry of Education, Key Open Laboratory of Flower Breeding and Bioengineering, National Forestry and Grassland Administration, Beijing Forestry University, Beijing 100083, China
2. College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China
3. Shandong Yate Eco-tech, Co., Ltd., Junan 266071, China

Received:2023-01-30 Revised:2024-10-24 Online:2025-01-30 Published:2025-01-21
Contact: LIU Zhonghua E-mail:hyacinth1107@163.com;liuzh6@bjfu.edu.cn

Abstract

Abstract:

【Objective】Chaenomeles speciosa has high ornamental, edible, and medicinal values. China is the origin and distribution center of C. speciosa. The genetic diversity of Chaenomeles speciosa germplasm resources was studied, and the molecular identity card of varieties was constructed to solve the problems of lack of unified classification criteria among varieties, homonymous and synoonymous, and unclear origin and evolution among varieties in recent years.【Method】A total of 168 C. speciosa varieties and SSR (simple sequence repeats) markers were combined with capillary electrophoresis to analyze the genetic diversity and the degree of genetic differentiation. The observed number of alleles (N_a), Shannon’s information index (I), and the polymorphism information content (PIC) were employed to screen primer combinations that can distinguish the entire germplasm and construct DNA molecular IDs based on string codes. 【Result】The results showed that 26 pairs of primers amplified 304 alleles in 168 C. speciosa varieties, with an average of 11.577 per locus. The average expected heterozygosity (H_e), I and PIC were determined as 0.748, 1.731 and 0.607, respectively. Based on the cluster analysis, the population could be divided into two groups, and further into six groups. Moreover, based on population structure analysis, the tested materials was divided into two subgroups. Four pairs of core primers were selected from 26 pairs of primers to construct barcode and two-dimensional code identification cards of C. speciosa varieties. 【Conclusion】The selected amplification loci have a high degree of variation and strong discrimination, and are thus suitable for applications in genetic diversity analysis, core primer screening, and fingerprint construction. The results can provide a reference for the variety identification, genetic resource management, and the construction of a germplasm resource database of C. speciosa.

Key words: Chaenomeles speciosa, genetic diversity, SSR, molecular ID

CLC Number:

LI Hui, HOU Lina, WANG Tianqi, BI Ningning, LI Shengbo, LIU Zhonghua. The genetic diversity analysis and molecular ID establishment of Chaenomeles speciosa based on SSR markers[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2025, 49(1): 59-68.

Figures/Tables 9

Supplemental Table 1

Sampling information of Chaenomeles speciosa"

编号 code	品种名 name of accessions	来源 source of collection	编号 code	品种名 name of accessions	来源 source of collection	编号 code	品种名 name of accessions	来源 source of collection
1	‘亚特020’ ‘Yate020’	山东临沂Linyi,Shandong	57	‘亚特084’ ‘Yate084’	山东临沂Linyi,Shandong	113	‘亚特094’ ‘Yate094’	山东临沂Linyi,Shandong
2	‘亚特108’ ‘Yate108’	山东临沂Linyi,Shandong	58	‘亚特288’ ‘Yate288’	山东临沂Linyi,Shandong	114	‘亚特332’ ‘Yate332’	贵州正安Zheng’an,Guizhou
3	‘亚特254’ ‘Yate254’	贵州正安Zheng’an,Guizhou	59	‘亚特343’ ‘Yate343’	云南大理Dali,Yunnan	115	‘亚特247’ ‘Yate247’	山东临沂Linyi,Shandong
4	‘亚特173’ ‘Yate173’	山东临沂Linyi,Shandong	60	‘亚特106’ ‘Yate106’	山东临沂Linyi,Shandong	116	‘亚特292’ ‘Yate292’	湖北恩施Enshi,Hubei
5	‘亚特265’ ‘Yate265’	云南大理Dali,Yunnan	61	‘亚特149’ ‘Yate149’	山东临沂Linyi,Shandong	117	‘亚特157’ ‘Yate157’	山东临沂Linyi,Shandong
6	‘亚特194’ ‘Yate194’	山东临沂Linyi,Shandong	62	‘亚特233’ ‘Yate233’	山东临沂Linyi,Shandong	118	‘亚特114’ ‘Yate114’	山东临沂Linyi,Shandong
7	‘亚特287’ ‘Yate287’	湖北恩施Enshi,Hubei	63	‘亚特246’ ‘Yate246’	山东临沂Linyi,Shandong	119	‘亚特380’ ‘Yate380’	贵州正安Zheng’an,Guizhou
8	‘亚特245’ ‘Yate245’	山东临沂Linyi,Shandong	64	‘亚特111’ ‘Yate111’	山东临沂Linyi,Shandong	120	‘亚特059’ ‘Yate059’	山东临沂Linyi,Shandong
9	‘亚特282’ ‘Yate282’	山东临沂Linyi,Shandong	65	‘绿香玉’ ‘Lü Xiangyu’	山东临沂Linyi,Shandong	121	‘亚特139’ ‘Yate139’	山东临沂Linyi,Shandong
10	金宝罗青’ ‘101Jinbaoluoqing101’	山东临沂Linyi,Shandong	66	‘亚特162’ ‘Yate162’	山东临沂Linyi,Shandong	122	‘亚特028’ ‘Yate028’	山东临沂Linyi,Shandong
11	‘亚特125’ ‘Yate125’	山东临沂Linyi,Shandong	67	‘亚特240’ ‘Yate240’	山东临沂Linyi,Shandong	123	‘亚特064’ ‘Yate064’	山东临沂Linyi,Shandong
12	‘亚特326’ ‘Yate326’	山东临沂Linyi,Shandong	68	‘亚特293’ ‘Yate293’	湖北恩施Enshi,Hubei	124	‘金宝罗青108’‘Jinbaoluoqing108’	山东临沂Linyi,Shandong
13	‘亚特115’ ‘Yate115’	山东临沂Linyi,Shandong	69	‘亚特095’ ‘Yate095’	山东临沂Linyi,Shandong	125	‘亚特172’ ‘Yate172’	山东临沂Linyi,Shandong
14	‘亚特069’ ‘Yate069’	山东临沂Linyi,Shandong	70	‘亚特359’ ‘Yate359’	云南大理Dali,Yunnan	126	‘亚特112’ ‘Yate112’	山东临沂Linyi,Shandong
15	‘亚特338’ ‘Yate338’	山东临沂Linyi,Shandong	71	‘亚特379’ ‘Yate379’	云南大理Dali,Yunnan	127	‘亚特074’ ‘Yate074’	山东临沂Linyi,Shandong
16	‘亚特206’ ‘Yate206’	山东临沂Linyi,Shandong	72	‘亚特053’ ‘Yate053’	山东临沂Linyi,Shandong	128	‘亚特215’ ‘Yate215’	山东临沂Linyi,Shandong
17	‘亚特177’ ‘Yate177’	山东临沂Linyi,Shandong	73	‘亚特050’ ‘Yate050’	山东临沂Linyi,Shandong	129	‘亚特381’ ‘Yate381’	贵州正安Zheng’an,Guizhou
18	‘亚特356’ ‘Yate356’	山东临沂Linyi,Shandong	74	‘亚特253’ ‘Yate253’	山东临沂Linyi,Shandong	130	‘亚特283’ ‘Yate283’	山东临沂Linyi,Shandong
19	‘亚特346’ ‘Yate346’	山东临沂Linyi,Shandong	75	‘亚特096’ ‘Yate096’	山东临沂Linyi,Shandong	131	‘亚特338’ ‘Yate338’	贵州正安Zheng’an,Guizhou
20	‘亚特231’ ‘Yate231’	山东临沂Linyi,Shandong	76	‘亚特302’ ‘Yate302’	云南大理Dali,Yunnan	132	‘亚特156’ ‘Yate156’	山东临沂Linyi,Shandong
21	‘亚特182’ ‘Yate182’	山东临沂Linyi,Shandong	77	‘亚特351’ ‘Yate351’	云南大理Dali,Yunnan	133	‘亚特035’ ‘Yate035’	山东临沂Linyi,Shandong
22	‘黄香玉’ ‘Huang Xinagyu’	山东临沂Linyi,Shandong	78	‘亚特229’ ‘Yate229’	山东临沂Linyi,Shandong	134	‘亚特024’ ‘Yate024’	山东临沂Linyi,Shandong
23	‘亚特322’ ‘Yate322’	山东临沂Linyi,Shandong	79	‘亚特092’ ‘Yate092’	山东临沂Linyi,Shandong	135	‘亚特058’ ‘Yate058’	山东临沂Linyi,Shandong
24	‘金宝罗青104’ ‘Jinbaoluoqing104’	山东临沂Linyi,Shandong	80	‘亚特301’ ‘Yate301’	云南大理Dali,Yunnan	136	‘亚特197’ ‘Yate197’	山东临沂Linyi,Shandong
25	‘红香玉’ ‘Hong Xiangyu’	山东临沂Linyi,Shandong	81	‘亚特076’ ‘Yate076’	山东临沂Linyi,Shandong	137	‘亚特122’ ‘Yate122’	山东临沂Linyi,Shandong
26	‘亚特339’ ‘Yate339’	山东临沂Linyi,Shandong	82	‘亚特060’ ‘Yate060’	山东临沂Linyi,Shandong	138	‘亚特327’ ‘Yate327’	贵州正安Zheng’an,Guizhou
27	‘亚特041’ ‘Yate041’	山东临沂Linyi,Shandong	83	‘亚特328’ ‘Yate328’	云南大理Dali,Yunnan	139	‘亚特057’ ‘Yate057’	山东临沂Linyi,Shandong
28	‘亚特178’ ‘Yate178’	山东临沂Linyi,Shandong	84	‘亚特284’ ‘Yate284’	湖北恩施Enshi,Hubei	140	‘亚特073’ ‘Yate073’	山东临沂Linyi,Shandong
29	‘金宝罗青103’ ‘Jinbaoluoqing103’	山东临沂Linyi,Shandong	85	‘亚特003’ ‘Yate003’	山东临沂Linyi,Shandong	141	亚特398’ ‘Yate398	贵州正安Zheng’an,Guizhou
30	‘亚特376’ ‘Yate376’	湖北恩施Enshi,Hubei	86	‘亚特187’ ‘Yate187’	山东临沂Linyi,Shandong	142	亚特090’ ‘Yate090	山东临沂Linyi,Shandong
31	‘金宝罗青106’ ‘Jinbaoluoqing106’	山东临沂Linyi,Shandong	87	‘亚特242’ ‘Yate242’	山东临沂Linyi,Shandong	143	亚特123’ ‘Yate123	山东临沂Linyi,Shandong
32	‘青香玉’ ‘Qing Xiangyu’	山东临沂Linyi,Shandong	88	‘亚特170’ ‘Yate170’	山东临沂Linyi,Shandong	144	亚特317’ ‘Yate317	贵州正安Zheng’an,Guizhou
33	‘亚特394’ ‘Yate394’	湖北长阳Changyang,Hubei	89	‘亚特006’ ‘Yate006’	山东临沂Linyi,Shandong	145	亚特311’ ‘Yate311	贵州正安Zheng’an,Guizhou
34	‘亚特280’ ‘Yate280’	山东临沂Linyi,Shandong	90	‘亚特047’ ‘Yate047’	山东临沂Linyi,Shandong	146	亚特375’ ‘Yate375	贵州正安Zheng’an,Guizhou
35	‘亚特322’ ‘Yate322’	云南大理Dali,Yunnan	91	‘亚特307’ ‘Yate307’	云南大理Dali,Yunnan	147	亚特333’ ‘Yate333	贵州正安Zheng’an,Guizhou
36	‘亚特196’ ‘Yate196’	山东临沂Linyi,Shandong	92	‘亚特347’ ‘Yate347’	云南大理Dali,Yunnan	148	亚特099’ ‘Yate099	山东临沂Linyi,Shandong
37	‘亚特313’ ‘Yate313’	云南大理Dali,Yunnan	93	‘亚特337’ ‘Yate337’	云南大理Dali,Yunnan	149	亚特089’ ‘Yate089	山东临沂Linyi,Shandong
38	‘亚特007’ ‘Yate007’	山东临沂Linyi,Shandong	94	‘亚特220’ ‘Yate220’	山东临沂Linyi,Shandong	150	亚特055’ ‘Yate055	山东临沂Linyi,Shandong
39	‘亚特390’ ‘Yate390’	云南大理Dali,Yunnan	95	‘亚特285’ ‘Yate285’	山东临沂Linyi,Shandong	151	‘亚特298’ ‘Yate298’	湖北恩施Enshi,Hubei
40	‘亚特093’ ‘Yate093’	山东临沂Linyi,Shandong	96	‘亚特146’ ‘Yate146’	山东临沂Linyi,Shandong	152	‘亚特102’ ‘Yate102’	山东临沂Linyi,Shandong
41	‘亚特063’ ‘Yate063’	山东临沂Linyi,Shandong	97	‘亚特022’ ‘Yate022’	山东临沂Linyi,Shandong	153	‘亚特205’ ‘Yate205’	山东临沂Linyi,Shandong
42	‘亚特077’ ‘Yate077’	山东临沂Linyi,Shandong	98	‘亚特353’ ‘Yate353’	贵州正安Zheng’an,Guizhou	154	‘亚特179’ ‘Yate179’	山东临沂Linyi,Shandong
43	‘京01’ ‘Jing01’	北京Beijing	99	‘亚特039’ ‘Yate039’	山东临沂Linyi,Shandong	155	‘亚特382’ ‘Yate382’	贵州正安Zheng’an,Guizhou
44	‘京02’ ‘Jing02’	北京Beijing	100	‘亚特043’ ‘Yate043’	山东临沂Linyi,Shandong	156	‘亚特009’ ‘Yate009’	山东临沂Linyi,Shandong
45	‘京03’ ‘Jing03’	北京Beijing	101	‘亚特100’ ‘Yate100’	山东临沂Linyi,Shandong	157	‘亚特391’ ‘Yate391’	贵州正安Zheng’an,Guizhou
46	‘苏01’ ‘Su01’	江苏苏州Suzhou,Jiangsu	102	‘金香玉’ ‘Jin Xiangyu’	山东临沂Linyi,Shandong	158	‘亚特268’ ‘Yate268’	云南大理Dali,Yunnan
47	‘苏02’ ‘Su02’	江苏苏州Suzhou,Jiangsu	103	‘亚特251’ ‘Yate251’	山东临沂Linyi,Shandong	159	‘亚特201’ ‘Yate201’	山东临沂Linyi,Shandong
48	‘亚特021’ ‘Yate021’	山东临沂Linyi,Shandong	104	‘亚特124’ ‘Yate124’	山东临沂Linyi,Shandong	160	‘亚特127’ ‘Yate127’	山东临沂Linyi,Shandong
49	‘亚特176’ ‘Yate176’	山东临沂Linyi,Shandong	105	‘亚特045’ ‘Yate045’	山东临沂Linyi,Shandong	161	‘亚特117’ ‘Yate117’	山东临沂Linyi,Shandong
50	‘贵02’ ‘Gui02’	贵州贵阳Guiyang,Guizhou	106	‘亚特227’ ‘Yate227’	山东临沂Linyi,Shandong	162	‘亚特305’ ‘Yate305’	山东临沂Linyi,Shandong
51	‘贵01’ ‘Gui01’	贵州贵阳Guiyang,Guizhou	107	‘亚特334’ ‘Yate334’	贵州正安Zheng’an,Guizhou	163	‘亚特164’ ‘Yate164’	山东临沂Linyi,Shandong
52	‘金宝罗青102’ ‘Jinbaoluoqing102’	山东临沂Linyi,Shandong	108	‘亚特226’ ‘Yate226’	山东临沂Linyi,Shandong	164	‘亚特116’ ‘Yate116’	山东临沂Linyi,Shandong
53	‘贵03’ ‘Gui03’	贵州贵阳Guiyang,Guizhou	109	‘亚特036’ ‘Yate036’	山东临沂Linyi,Shandong	165	‘亚特286’ ‘Yate286’	湖北恩施Enshi,Hubei
54	‘亚特088’ ‘Yate088’	山东临沂Linyi,Shandong	110	‘亚特044’ ‘Yate044’	山东临沂Linyi,Shandong	166	‘亚特350’ ‘Yate350’	山东临沂Linyi,Shandong
55	‘亚特203’ ‘Yate203’	山东临沂Linyi,Shandong	111	‘亚特005’ ‘Yate005’	山东临沂Linyi,Shandong	167	‘亚特281’ ‘Yate281’	山东临沂Linyi,Shandong
56	‘亚特048’ ‘Yate048’	山东临沂Linyi,Shandong	112	‘亚特318’ ‘Yate318’	贵州正安Zheng’an,Guizhou	168	‘亚特012’ ‘Yate012’	山东临沂Linyi,Shandong

Supplemental Table 1

Fig. 1

Table 1

Polymorphism of SSR primers at 26 loci"

引物 locus	类型 type	重复基序 repeat motif	荧光标记 fluorescent	N_a	N_e	H_e	I	H	PIC	引物序列 primer sequence
Nak1	p3	(TTC)6	FAM	12	2.857	0.651	1.508	0.650	0.455	F:GCGGGCGCGGTTTTATAATT R:GGCACACTGCTAGGGTTTCT
Nak2	p2	(CT)8	TAMRA	17	4.506	0.780	1.921	0.778	0.612	F:TCTCAGAGTCCTCTCTCTTTCCT R:GGAGGGCCATTATCCTCTGC
Nak3	p2	(AG)7	ROX	13	6.362	0.845	2.043	0.843	0.769	F:GGTGGTTACTCTGCCGACTG R:TCGCTGGCTCCCTCATTTTC
Nak4	p2	(GA)6	HEX	13	2.061	0.516	1.292	0.515	0.287	F:CTTGGATCTGGGCGAAGAGT R:CCACCTCTGACACTGGAACC
Nak5	p3	(GGA)6	HEX	13	6.277	0.843	2.060	0.841	0.812	F:GAGAGAACGAGATGCCGTCC R:TCAACAGTGGGTTCGCACAG
Nak6	p3	(TCC)8	TAMRA	8	2.685	0.629	1.301	0.628	0.444	F:TCATTCGGATCCTCCCACCT R:ACACGGAAACAGAGTCTGCA
Nak7	p3	(TTG)7	ROX	14	2.406	0.586	1.405	0.584	0.324	F:GCTGAGAGAGGTCGATGAGC R:AGAAACTTCAGCAGCGACCA
Nak8	p3	(AGA)8	FAM	11	4.881	0.798	1.879	0.795	0.662	F:TGCAAGTCTACAGCAGCAGG R:TTAAGGTTACCCGACGGTGC
Nak9	p3	(GGC)6	HEX	10	4.979	0.802	1.786	0.799	0.694	F:AACATGCCGGGACCATCTTT R:AACATGCCGGGACCATCTTT
Nak10	p3	(GGA)5	HEX	18	6.042	0.837	2.139	0.835	0.725	F:GGCGAGAGGGTAGTAGTGGA R:GGCGAGAGGGTAGTAGTGGA
Nak11	p3	(GCA)6	FAM	9	3.946	0.749	1.709	0.747	0.592	F:CTGGACATTCGAGTGAGCCA R:ATTCCAGTTTCAATGGCGGC
Nak12	p3	(TGC)7	FAM	10	4.983	0.802	1.883	0.799	0.666	F:CGGTGGCAAGGAAAGAGGAT R:ATCAGAGGGTTGTTTCGCGT
Nak13	p2	(TC)9	FAM	8	3.317	0.701	1.451	0.699	0.440	F:GCTCGTCCTAGAGGGCAAAG R:CATCCTGTAACCATCGGCCA
Nak14	p2	(CT)12	FAM	12	4.589	0.784	1.768	0.782	0.616	F:TACCGCTCTCTCCCTCTCTC R:ATAGGCCCGTCAAACTCGAC
Nak15	p2	(GA)7	TAMRA	15	6.766	0.855	2.217	0.852	0.762	F:CAGGGGTCAGTCAGCGAAAG R:CCTACACTTGCTGACCGGTT
Nak16	p2	(TC)20	TAMRA	16	5.847	0.832	2.045	0.829	0.694	F:ACATGGGCTCCTAGGTACGT R:TTTCGTCTTGCCGTCCATCA
Nak17	p3	(ACC)6	HEX	14	5.813	0.832	2.037	0.828	0.751	F:ATTGTTGGTGAGAGGGCTGG R:GCAGCTGGTGGTGGCTATTA
Nak18	p3	(TCC)8	FAM	15	4.146	0.763	1.923	0.759	0.583	F:TACCGCAAAACCTCAGCTCC R:AACCACACTGCGGAGTTCAT
Nak19	p3	(CGA)6	FAM	9	2.332	0.574	1.202	0.571	0.503	F:GCGGGTTCGACGACAGTTAT R:ACACACTTGCTACGTACGGA
Nak20	p3	(TTC)7	HEX	13	3.505	0.719	1.598	0.715	0.562	F:TTCCAAACCCAAACTCAGATTTT R:TGCGTTCTGTGTGTCCTCTC
Nak21	p3	(AAT)6	TAMRA	12	4.332	0.776	1.812	0.769	0.695	F: GAGGGACCTAGCTGTGTGTG R:GCCCAACATCACTGTTCAGC
Nak22	p2	(TA)6	TAMRA	7	2.710	0.639	1.323	0.631	0.427	F: AGAAGGGTGAGGGGTCTTGA R:CCACCTCCACAATCCTCCTC
Nak23	p2	(TC)9	TAMRA	10	4.694	0.793	1.845	0.787	0.656	F: ACTTGCAGATGGTTGCCCTT R:CAGGGATGGAGATTCGGACG
Nak24	p2	(TA)8	TAMRA	9	5.929	0.837	1.921	0.831	0.767	F: GGTCGTGACGCCTCGATATT R:CGCGGCCTACGTTCATCTTA
Nak25	p2	(GA)12	ROX	9	3.925	0.751	1.566	0.745	0.599	F: GGTGTGGTGAAGGAGACTGG R:GACCCTTGGATTGGCGAAGA
Nak26	P3	(AAC)6	ROX	4	3.895	0.751	1.373	0.743	0.696	F: CCTTGAGTGACCCCTTCAGC R:AGCCGGCACTCCAAATGTTA
均值mean				11.577	4.376	0.748	1.731	0.744	0.607

Table 1

Fig. 2

Fig. 3

Table 2

Table 3

Alleles selection and encoded standard"

引物 primer	N_a	毛细管电泳多态性片段编码 code of capillary electrophoresis polymorphism fragments
Nak2	16	215(1)、217(2)、217/219(3)、217/225(4)、217/229(5)、217/231(6)、219(7)、219/221(8)、219/225(9)、219/227(A)、219/235(B)、221(C)、221/227(D)、221/235(E)、221/238(F)、237(G)
Nak3	24	133(1)、191(2)、191/201(3)、191/205(4)、193(5)、193/195(6)、193/197(7)、193/199(8)、193/201(9)、193/203(A)、195(B)、195/197(C)、195/199(D)、195/201(E)、195/203(F)、197(G)、197/199(H)、197/201(I)、197/203(J)、199(K)、199/201(L)、199/203(M)、201(N)、203(O)
Nak5	26	177(1)、177/187(2)、177/189(3)、177/191(4)、179(5)、179/189(6)、179/191(7)、179/193(8)、179/195(9)、181/191(A)、181/195(B)、187(C)、187/191(D)、187/193(E)、189(F)、189/191(G)、189/193(H)、189/195(I)、191(J)、191/193(K)、191/195(L)、191/199(M)、193(N)、193/195(O)、195(P)、197(Q)
Nak7	17	125(1)、125/131(2)、125/134(3)、128(4)、128/131(5)、128/134(6)、128/137(7)、128/140(8)、131(9)、131/134(A)、131/137(B)、134(C)、134/137(D)、137(E)、137/143(F)、143(G)、146(H)
Nak8	14	138(1)、138/144(2)、141(3)、141/144(4)、141/147(5)、144(6)、144/147(7)、147(8)、147/165(9)、147/168(A)、162(B)、162/165(C)、165(D)、168(E)
Nak10	24	116(1)、119/128(2)、122(3)、122/125(4)、122/128(5)、122/131(6)、122/134(7)、122/173(8)、125(9)、125/128(A)、125/131(B)、125/134(C)、125/176(D)、128(E)、128/134(F)、131(G)、131/137(H)、131/158(I)、134(J)、134/155(K)、137/155(L)、152(M)、158(N)、158/161(O)
Nak11	13	137/143(1)、137/146(2)、137/149(3)、140(4)、140/143(5)、140/146(6)、140/149(7)、143(8)、143/146(9)、143/149(A)、146(B)、146/149(C)、149(D)
Nak12	15	214(1)、217(2)、217/220(3)、217/223(4)、217/229(5)、220/223(6)、220/229(7)、220/232(8)、223(9)、223/229(A)、223/232(B)、223/235(C)、226(D)、229(E)、232(F)
Nak14	12	137(1)、137/146(2)、137/149(3)、137/152(4)、143/149(5)、143/152(6)、146(7)、146/149(8)、146/152(9)、149(A)、149/152(B)、152(C)
Nak17	23	215(1)、215/239(2)、215/245(3)、217(4)、217/219(5)、217/233(6)、217/235(7)、217/237(8)、217/239(9)、217/241(A)、217/243(B)、219(C)、219/233(D)、219/241(E)、221(F)、221/235(G)、233(H)、233/241(I)、235(J)、235/241(K)、239(L)、241(M)、247(N)
Nak25	9	179(1)、179/185(2)、179/239(3)、179/251(4)、237/251(5)、238/251(6)、239(7)、239/251(8)、251(9)

Table 3

Table 4

Fingerprint database code of Chaenomeles speciosa germplasms"

编号 No.	分子身份证编码 molecular ID	编号 No.	分子身份证编码 molecular ID	编号 No.	分子身份证编码 molecular ID	编号 No.	分子身份证编码 molecular ID
1	7FF9657E913	43	68557L568G0	85	25FH66C5CM0	127	AJFC8E9E440
2	BO42D6C2A07	44	58556K268F0	86	39H06B958I0	128	7ELC892E748
3	CBJA6367C24	45	7N59CG63343	87	79FG649E801	129	8EA9099EBA8
4	8FE5632E714	46	34H82151C08	88	39HG649E941	130	8BLD3935AC0
5	8B866365B35	47	34H82151909	89	7GPC8CBD700	131	7GQC8JB9700
6	CFEA656ECM8	48	38NC63C5C69	90	85LB8A1FC00	132	7OPC29B3C04
7	DN16B647A32	49	25N96GB97N3	91	25P96CB97N0	133	CMLD39B5B49
8	CFEC13BEBL1	50	75266327843	92	7GPC8NBD708	134	7M9E8A97B00
9	3BMA2335AD4	51	A8CA6BCAAF0	93	35HA1DAEC68	135	CML1899E900
10	753664978E0	52	32J403B9B00	94	3ONA69959M0	136	8JPE8EBE900
11	35HA64C7941	53	3HFA139E940	95	39HD649E9A0	137	8E078A2EC90
12	29HA649E9A7	54	4945438EB40	96	79HC669EBA8	138	8EBD599E990
13	3E6564177E0	55	39DA649ECA0	97	8FL939BBC08	139	CBAB592E960
14	278364B7BK0	56	79DC13BEBM0	98	7BQC8JBD7N0	140	8EJD59959A0
15	35FA13AE960	57	CAIB9A97240	99	15P96MB97N0	141	CELD599E940
16	3NE66365A68	58	75ND5AC5CH0	100	8FLE39BFB00	142	EOBB3955BA0
17	33CA6527B48	59	75JC5AC5CM0	101	AEA1D9BF200	143	784B6C8EB43
18	75266317851	60	7BP98JB97N0	102	8EPB491FC00	144	780B698EBA3
19	7L36B725484	61	89KD8A9E9A0	103	CBLE8A2F800	145	38HB649E6A0
20	4K2564B3C87	62	39KB8ADE900	104	8FLB1D9FB00	146	35NB1335A00
21	35C214A3B47	63	7NN969B3CD0	105	77FCD372200	147	38EE649E9A0
22	353A632EB40	64	89KD8A9E9A0	106	25P96OB9700	148	77DB13CEB49
23	75D66487750	65	FDJE825EB40	107	28G968CEC00	149	37I96IB4970
24	25N96GB97N0	66	7FIC59957A0	108	9KG968BDB04	150	25E96GBA7N0
25	2KF9631E840	67	7OPC89B3CD0	109	7HGB6395C00	151	2N7B6487749
26	8AHB8A9E904	68	7BQC8NB97J0	110	39JD739FC00	152	78EC64974N9
27	CBNB3935A04	69	7BQC8JB97N0	111	3KHB649F100	153	CNND89C5AI0
28	7BF95AC5C04	70	8FLD8A9E9B0	112	25F923C8203	154	CJ758A87B40
29	7B658A8EB00	71	AMJC8E9E440	113	35HE69BF900	155	89KB8917850
30	8BP98NB9800	72	CFPE89B5CI8	114	3NGD638E400	156	7BPC8NB97J9
31	CBJA3935808	73	31HD13CF908	115	35F5638EB03	157	7BPC8JB97N0
32	7FNA29C5B00	74	25L96GB9704	116	2NF91335CD8	158	CFNE8A9E9A0
33	7BQ98AB97J0	75	79HE13BFB07	117	75FE198FB08	159	89KB8A9E9A0
34	EF7BE95E840	76	756AD955A47	118	7NGC24B7B00	160	75JB39CEC40
35	CFLB8A87750	77	25P966BD707	119	3NFB83B8C00	161	8MA99FB7200
36	2BJC8JB97N7	78	25P96NB9709	120	B8G96BB5940	162	8BLD8E95CI0
37	EDL78CB5BA7	79	46HA659C809	121	7B9E392EBE0	163	CKLCAF63BA1
38	CBOE8A9EB09	80	75H6656E900	122	CFJD8ABE8I0	164	8DBA9A87BL0
39	CFJE5997749	81	7IEF2H9E508	123	CELD8A2E754	165	7DJC39CEBM0
40	7DLC8ACECA8	82	25PH8GB9708	124	CB9B89877C1	166	7EPE3AB3BE0
41	25P96GB97N8	83	7NNG6HB8C00	125	8B9A892EBC1	167	CBA78917850
42	G8NA649E9A0	84	29HE1IB59M0	126	AC9A9E97B40	168	COJE8AB5C40

Table 4

Fig. 4

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减少的引物 name of reduced primers	鉴别率/% discrimination rate	不能区分的种质 the indistinguished germplasm
减少的引物 name of reduced primers	鉴别率/% discrimination rate	种质数量 number	种质编号 No. of C. speciosa germplasm
Nak1、Nak4、Nak6、Nak13、Nak22	100	0	——
Nak9、Nak19、Nak23、Nak24、Nak26	100	0	——
Nak15、Nak16、Nak18、Nak20、Nak21	100	0
Nak8、Nak11、Nak12、Nak25	90.48	16	27、31、41、49、55、61、67、74、78、95、132、137、138、145、147、159
Nak14、Nak21	82.74	29	15、24、27、31、33、41、46、47、49、55、60、61、62、67、74、78、80、93、95、115、132、137、138、145、147、156、157、159、160
Nak3、Nak5	57.74	71	1、15、24、27、31、33、36、38、39、41、46、47、48、49、53、55、56、60、61、62、63、64、67、68、69、71、72、73、74、78、80、87、88、89、91、92、93、95、96、98、100、102、106、110、111、113、115、121、122、127、128、129、131、132、133、137、138、144、145、146、147、148、153、154、156、157、159、160、165、166、168

The genetic diversity analysis and molecular ID establishment of Chaenomeles speciosa based on SSR markers

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