The influence of arbuscular mycorrhizal fungi (AMF) inoculation on the growth status and photosynthetic characteristics of Leucojum aestivum

doi:10.12302/j.issn.1000-2006.202309012

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2025, Vol. 49 ›› Issue (3): 205-212.doi: 10.12302/j.issn.1000-2006.202309012

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The influence of arbuscular mycorrhizal fungi (AMF) inoculation on the growth status and photosynthetic characteristics of Leucojum aestivum

WANG Wei¹(), MU Hongna¹^,²^,^*(), YANG Huimin¹, SUN Taoze¹

1. College of Horticulture and Gardening, Yangtze University, Jingzhou 434025, China
2. The Engineering Research Center of Ecology and Agricultural Use of Wetland, Ministry of Education, Yangtze University, Jingzhou 434025, China

Received:2023-09-08 Accepted:2024-12-03 Online:2025-05-30 Published:2025-05-27
Contact: MU Hongna E-mail:2021720877@yangtzeu.edu.cn;hongnamu@yangtzeu.edu.cn

Abstract

Abstract:

【Objective】This study investigated the effects of arbuscular mycorrhizal fungi (AMF) colonization on the growth and photosynthetic characteristics of Leucojum aestivum. 【Method】Two-year-old bulbils of L. aestivum were selected as the experimental material. The experimental design consisted of five groups, three of which received single AMF inoculations [inoculated with Rhizophagus intraradices (Ri), Funneliformis mosseae (Fm), and Diversispora tortuosa (Dt)]. The fourth group was inoculated with a 1∶1∶1 mixture of the three AMF species (Mi), and the control group was inoculated with a sterilized 1∶1∶1 mixture of the AMFs. After two months, plant height, fresh and dry weight, and photosynthetic characteristics of L. aestivum were measured. 【Result】The Fm group exhibited the highest infection rate at 69.38%, while the Ri group had the lowest infection rate at 36.93%. The infection rate in the Ri group was significantly lower than those of the Fm, Dt, and Mi groups. Among the treatment groups, the Fm group showed the most plant height (43.46 cm) and the dry weight (39.94 g). Photosynthetic parameters indicated that the Ri group had the most average stomatal conductance [Gs = 0.30 mol/(m²·s), intercellular CO₂ concentration (Ci = 331 μmol/mol), and transpiration rate (Tr = 3.45 mmol/(m²·s), with the transpiration rate significantly higher than in the control group (CK)]. Correlation analysis revealed a generally significant positive correlation between net photosynthetic rate (Pn) and Gs across all treatments, except for leaf stomatal conductance (Ls) and Ci. Although other photosynthetic indices exhibited correlations, no consistent pattern was observed across treatments. Membership function analysis indicated that the inoculation effect of the Mi group was the most beneficial, followed by Dt. 【Conclusion】AMF colonization effectively promotes the growth of L. aestivum. The effects on photosynthesis vary across different AMF species, with Dt and Mi significantly enhancing the photosynthetic capacity and biomass of L. aestivum. In contrast, Fm positively affected plant height but did not significantly influence photosynthetic capacity.

Key words: Leucojum aestivum., arbuscular mycorrhizal fungi (AMF), growth status, photosynthetic characteristics, correlation analysis, membership function method

CLC Number:

S718

WANG Wei, MU Hongna, YANG Huimin, SUN Taoze. The influence of arbuscular mycorrhizal fungi (AMF) inoculation on the growth status and photosynthetic characteristics of Leucojum aestivum[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2025, 49(3): 205-212.

Figures/Tables 7

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Table 1

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处理 treatment	株高/cm plant height
处理 treatment	77 d	105 d	131 d	146 d
Ri	4.74±1.84 a	13.24±5.07 a	23.63±4.86 a	39.23±3.40 ab
Fm	5.83±0.63 a	13.09±2.26 a	27.33±5.26 a	43.46±2.92 a
Dt	5.59±2.01 a	14.51±4.51 a	25.89±6.06 a	42.56±4.23 a
Mi	5.23±1.57 a	12.77±3.76 a	22.73±5.80 a	40.24±3.75 ab
CK	4.69±1.53 a	10.76±3.95 a	21.84±6.91 a	36.18±5.49 b

处理组 treatment	鲜质量/g fresh weight			干质量/g dry weight
处理组 treatment	地上部 ground	球茎 corm	根 root	地上部 ground	球茎 corm	根 root
Ri	22.46±6.34 ab	52.33±14.47 ab	38.82±2.75 a	2.91±0.82 a	32.81±9.27 a	2.91±0.03 ab
Fm	12.36±0.78 b	55.57±4.82 ab	42.51±3.81 a	2.90±0.60 a	33.68±1.89 a	3.37±0.75 ab
Dt	27.58±4.30 ab	42.23±1.21 b	32.54±0.67 a	2.80±0.14 a	27.16±0.20 a	2.48±0.12 b
Mi	25.23±4.39 ab	48.46±8.42 ab	37.19±14.14 a	2.81±0.62 a	26.04±7.16 a	2.73±0.99 ab
CK	22.60±0.87 a	50.98±1.11 a	38.06±1.51 a	2.61±0.06 a	32.36±1.12 a	3.16±0.06 a

处理组 treatment	隶属值1 X1	隶属值2 X2	隶属值3 X3	隶属度(D) affiliation	排序 rank
Ri	0.000	0.395	0.218	0.111	5
Fm	0.318	0.507	0.000	0.301	4
Dt	1.000	0.045	0.141	0.675	2
Mi	0.777	1.000	0.718	0.809	1
CK	0.216	0.000	1.000	0.306	3

The influence of arbuscular mycorrhizal fungi (AMF) inoculation on the growth status and photosynthetic characteristics of Leucojum aestivum

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