JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2009, Vol. 33 ›› Issue (02): 5-.doi: 10.3969/j.jssn.1000-2006.2009.02.002

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Construction and analysis of Populus deltoides SSH cDNA library induced by Tetra lobulifera (Keifer)

ZHANG Ping, PAN Huixin, HUANG Minren*, WANG Mingxiu   

  1. Nanjing Forestry University, Jiangsu Key Laboratory for Poplar Germplasm Enhancement and Variety Improvement, Nanjing 210037, China
  • Online:2009-04-18 Published:2009-04-18

Abstract: A suppression subtractive hybridization (SSH) cDNA library was constructed with Populus deltoides induced by Tetra lobulifera (Keifer), the leaf was induced for 48 and 72 hours and used as the tester, whereas the induced leaf was employed as driver. Seven hundreds and ten cDNA clones were identified. PCR examination showed that most of the inserted fragments were 200—1 000 bp. The BlastN homology analysis revealed that these genes played varies roles in signal transduction, energy metabolism, response to stress and transportation etc.

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