Identification and genetic diversity analysis of Camellia oleifera varieties using ISSR marker

YU Xiaoyu, YU Fangyuan, LIU Jianbing, CHEN Junyong

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2013, Vol. 37 ›› Issue (01) : 61-66.

PDF(2056173 KB)
南京林业大学学报（自然科学版）
PDF(2056173 KB)
JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2013, Vol. 37 ›› Issue (01) : 61-66. DOI: 10.3969/j.issn.1000-2006.2013.01.010

Identification and genetic diversity analysis of Camellia oleifera varieties using ISSR marker

  • YU Xiaoyu1, YU Fangyuan1*, LIU Jianbing2, CHEN Junyong3
Author information +
History +

Abstract

Molecular marker is one of the important ways for precise identification of Camellia oleifera varieties. The genetic diversity of 66 varieties of C. oleifera which were from Hunan and Hubei provinces was investigated using ISSR marker to reveal the affinity of these varieties. The results showed that: 6 ISSR primers selected from 100 primers were employed to conduct PCR amplification on genome DNA of 66 varieties of cultivated C. oleifera. And a total of 121 countable bands were amplified. Among them, the number of polymorphic bands(NPB)were 112, and percentage of polymorphic bands(PPB)was 92.56%. The average value of observed number of alleles, average value of effective number of alleles, Nei’s gene diversity and Shannon’s information index were 1.933 9,1.650 9, 0.369 8 and 0.541 7 respectively. The above parameters indicated that the level of genetic diversity in different varieties of C. oleifera was high. Different varieties of C. oleifera could be identified according to whether the polymorphic bands exist or not, the number of polymorphic bands, and the type of polymorphic bands amplified by different primers.

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations
YU Xiaoyu, YU Fangyuan, LIU Jianbing, CHEN Junyong. Identification and genetic diversity analysis of Camellia oleifera varieties using ISSR marker[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2013, 37(01): 61-66 https://doi.org/10.3969/j.issn.1000-2006.2013.01.010

References

[1] 雷治国,黄永芳,何会蓉. 油茶及其种质资源研究进展[J]. 经济林研究,2003,21(4):123-125. Lei Z G, Huang Y F, He H R. Study on Camellia oleifera and its germ plasm resources[J]. Nonwood Forest Researches, 2003,21(4):123-125.
[2] 黄永芳,陈锡,庄雪影,等. 油茶种质资源遗传多样性分析[J]. 林业科学,2006,42(4):38-43. Huang Y F, Chen X, Zhuang X Y, et al. Analysis of genetic diversity in camellia oleifera germplasms[J]. Scientia Silvae Sinicae,2006,42(4):38-43.
[3] 周延清,李敏,贾敬芬,等. 河南大豆遗传多样性的ISSR分析[J]. 西北植物学报,2006,26(9):1883-1887. Zhou Y Q, Li M, Jia J F, et al. ISSR Analysis of genetic Diversity of soybean in Henan[J]. Acta Botanica Boreali-Occidentalia Sinica, 2006,26(9):1883-1887.
[4] Ziekiewicz E, Rafalski A, Labuda D. Genome fingerprinting by simple sequence repeat(SSR)-anchored polymerase chain reaction amplification[J]. Genomics,1994,20:176-183.
[5] 景润春,何予卿,黄青阳,等. 水稻野败型细胞质雄性不育恢复基因的ISSR和SSLP标记分析[J]. 中国农业科学,2000,33(2):10-15. Jing Y C, He Y Q, Huang Q Y, et al. Analysis of the fertilily restorer gene in the wild-Abortive(WA)type cytoplasmic male sterility(CMS)system with the ISSR and SSLP markers[J]. Scientia Agricultura Since, 2000,33(2):10-15.
[6] 李进波,江良容,李春海,等. 水稻光温敏核不育系ISSR和SSR遗传分析比较[J]. 分子植物育种,2003,1(1):42-47. Li J B, Jiang L R,Li C H, et al. Comparisons of genetic analysis based on ISSR and SSR in PGMS and TGMS rice lines[J]. Molecular Plant Breeding, 2003,1(1):42-47.
[7] 王家保,王令霞,杜中军,等. 部分芒果品种亲缘关系的ISSR分析[J]. 园艺学报,2007, 34(1):87-92. Wang J B,Wang L X,Du Z J, et al. Analysis on the genetic relationship of some Mango(Mangifera indica L.)Germplasms by ISSR markers[J]. Acta Horticulturae Sinica, 2007, 34(1):87-92.
[8] 吴子龙,方连玉,王军,等. 15份葡萄种质亲缘关系的ISSR分析[J]. 果树学报,2006, 23(4):605-608. Wu Z L, Fang L Y, Wang J,et al. Analysis of genetic relationship of 15 Vitis germplasm resources by ISSR markers[J]. Journal of Fruit Science, 2006, 23(4):605-608.
[9] 罗光明,陈岩,李霞,等. 枳壳道地产区主流品种遗传多样性的分析[J]. 江西农业大学学报,2007,29(1):124-128. Luo G M,Chen Y, Li X, et al. ISSR analysis on the genetic diversity of citrus aurantium’ s main current species in the genuine producing region[J]. Acta Agriculturae Universitatis Jiangxiensis, 2007,29(1):124-128.
[10] 陈少瑜,杨恩,习学良,等. 云南主要核桃品种的ISSR分子鉴别[J]. 经济林研究,2006,24(4):41-45. Chen S Y, Yang E, Xi X L, et al. Molecular identif ication of main varieties in Juglans Sigillata Dode from Yunnan province by ISSR[J]. Nonwood Forest Research, 2006,24(4):41-45.
[11] 王永清,付燕,杨芩,等. 枇杷属植物遗传多样性的ISSR分析[J]. 林业科学,2010,64(4):49-57. Wang Y Q, Fu Y, Yang Q, et al. Genetic diversity of Eriobotrya analyzed by ISSR markers[J]. Scientia Silvae Sinicae, 2010,64(4):49-57.
[12] 王保明,陈永忠,谭晓风等. 应用ISSR分析油茶无性系的遗传多样性[J]. 东北林业大学学报,2008,36(6):19-23. Wang B M, Chen Y Z, Tan X F, et al. Genetic diversity of elite clones of Camellia oleifera by ISSR[J]. Journal of Northeast Forestry University, 2008,36(6):19-23.
[13] 温强,雷小林,叶金山,等. 油茶高产无性系的ISSR分子鉴别[J]. 中南林业科技大学学报,2008, 28(1):39-43. Wen Q, Lei X L, Ye J S, et al. Identification of Camellia oleifera superior clones by ISSR molecular markers[J]. Journal of Central South University of Forestry & Technology, 2008, 28(1):39-43.
[14] 张国武,钟文斌,乌云塔娜,等. 油茶优良无性系ISSR分子鉴别[J]. 林业科学研究,2007,20(2):278-282. Zhang G W, Zhong W B, Wu Yun Tanna, et al. Identif ication of oil tea( Camellia oleifera)superior clones by ISSR molecular marker[J]. Forest Research, 2007,20(2):278-282.
[15] 彭方仁,吴莺莺,郝明灼,等. 利用ISSR和SRAP标记分析油茶遗传多样性[J]. 南京林业大学学报:自然科学版,2012,36(5):19-25. Peng F R,Wu Y Y, Hao M Z,et al. Genetic diversity of Camellia oleifera using ISSR and SRAP markers[J]. Journal of Nanjing Forestry University:Natural Sciences Edition, 2012,36(5):19-25.
[16] 王凤格,赵久然,郭景伦,等. 比较三种DNA指纹分析方法在玉米品种纯度及真伪鉴定中的应用[J].分子植物育种, 2003,1(5):655-662. Wang F G, Zhao J R,Guo J L,et al. Comparison of three DNA fingerprint analyzing methods for maize cultivars’ identification[J]. Molecular Plant Breeding, 2003,1(5):655-662.
[17] 柳李旺,候喜林,龚义勤,等. 分子标记技术在蔬菜作物品种鉴定与纯度检测中的应用[J]. 分子植物育种, 2004, 2(4):563-568. Liu L W, Hou X L, Gong Y Q, et al.Application of molecular marker in variety identification and purity testing in vegetable crops[J]. Molecular Plant Breeding, 2004, 2(4):563-568.
[18] Matsumoto S, Kiriiwa Y, Takeda Y. Differentiation of Japanese green tea cultivars as revealed by RFLP analysis of phenylianine ammonia-lyase DNA[J]. Theoretical and Applied Genetics, 2002, 104(6-7):998-1002.
[19] Wachira F N, Tanaka J, Takeda Y. Genetic variation and differentiation in tea(Camellia sinensis)germplasm revealed by RAPD and AFLP variation[J]. J Hort Sci Biotechnol, 2001, 76(5):537-563.
[20] 陈永忠,张智俊,谭晓风. 油茶优良无性系的RAPD分了鉴别[J]. 中南林学院学报, 2005, 25(4):40-45. Chen Y Z, Zhang Z J, Tan X F. Identification of oil tea(Camellia oleifera)superior clones by RAPD molecular marker[J]. Journal of Central South Forestry University, 2005, 25(4):40-45.
[21] 王蕾,叶志云,蒋燚,等. 利用ISSR技术对优质红锥种质资源遗传多样性的分析[J]. 厦门大学学报:自然科学版,2006,45(增刊):91-94. Wang L, Ye Z Y, Jiang Y, et al. Genetic diversity of Castanopsis hystrix by ISSR Analysis[J]. Journal of Xiamen University:Natural Science, 2006,45(S1):91-94.
[22] Moreno S, Martin J P. Ortiz M. Inter-simple sequence repeats PCR for characterization of closely related grapevine germplasm[J]. Euphytica,l998,101(1):117-125.
[23] Fang D Q, Roose M L, Kmager R R, et al. Fingerprinting trifoliate orange germplasm accessions with isozymes, RFLPs and inter-simple sequence repeat markers[J]. Theoretical and Applied Genetics,1997,95(1-2):211-219.
PDF(2056173 KB)

Accesses

Citation

Detail
Sections
Recommended
The full text is translated into English by AI, aiming to facilitate reading and comprehension. The core content is subject to the explanation in Chinese.

Author

Reader

Reviewer

www.nldxb.njfu.edu.cn

Edited ＆ Published by Editorial Department of Nanjing Forestry University(Natural Sciences Edition)
Address： No.159 Longpan Road,Nanjing 210037 Jiangsu,P.R.China
E-mail ：xuebao@njfu.edu.cn , xuebao@njfu.com.cn
Telephone +86-25-85428247,85427076
Distributed by China International Book Trading Corporation P.O. Box 399, Beijing ,P.R. China

/

〈 〉