Cloning and expression analysis of WrbA gene from Picea wilsonii

doi:10.3969/j.issn.1000-2006.2014.04.007

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2014, Vol. 38 ›› Issue (04): 34-38.doi: 10.3969/j.issn.1000-2006.2014.04.007

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Cloning and expression analysis of WrbA gene from Picea wilsonii

ZHANG Tong,ZHOU Yanni,LI Changjiang,ZHANG Lingyun^*

Key Laboratory of Forest Silviculture and Conservation of the Ministry of Education,Beijing Forestry University,Beijing 100083,China

Online:2014-07-31 Published:2014-07-31

Abstract

Abstract: The tryptophan(W)repressor-binding protein(WrbA)is an important antioxidant enzyme which has been shown to play roles in a variety of adversities. In this study,PwWrbA cDNA was cloned by RACE-PCR method. Bioinformatics analysis showed that the full length of PwWrbA cDNA was 1 045 bp, including the ORF 651 bp, encoding 203 amino acids. The theoretical molecular weight was 21.80 ku and value of pI was 6.43.The ScanProsite online software showed that the N terminal 11-15 aa and the C terminal 112-165 aa were supposed to be the FMN binding sites. RT-qPCR and semi-quantitative RT-PCR analysis showed PwWrbA expressed in various tissues, while the highest expression level was in pollen. Meanwhile, the expression of PwWrbA increased gradually after stress treatment with NaCl and ABA.In contrast to NaCl and ABA treatment, the expression of PwWrbA decreased in early stage and increased at 6 h after H₂O₂ treatment. Our results suggested that PwWrbA was a new WrbA gene and played roles during different abiotic stresses.

CLC Number:

Q78
S792

ZHANG Tong,ZHOU Yanni,LI Changjiang,ZHANG Lingyun. Cloning and expression analysis of WrbA gene from Picea wilsonii[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2014, 38(04): 34-38.

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Cloning and expression analysis of WrbA gene from Picea wilsonii

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