Spray drying processes of Bacillus valeriana YH-18

doi:10.3969/j.issn.1000-2006.201903012

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2020, Vol. 44 ›› Issue (5): 209-214.doi: 10.3969/j.issn.1000-2006.201903012

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Spray drying processes of Bacillus valeriana YH-18

WEI Danping(), YE Jianren^*(), LIANG Maojin, CHEN Feifei

Co-Innovation Center for the Sustainable Forestry in Southern China, Jiangsu Key Laboratory for Prevention and Management of Invasive Species, College of Forestry, Nanjing Forestry University, Nanjing 210037, China

Received:2019-03-05 Revised:2019-09-11 Online:2020-10-30 Published:2020-10-30
Contact: YE Jianren E-mail:1744308547@qq.com;jrye@njfu.com.cn

Abstract

Abstract:

【Objective】Bacillus valeriana YH-18 is a bacterial strain that exhibits an excellent antifungal ability in different kinds of trees. This strain is used as a biological agent in the form of bacterial powder. In this study, the spray drying of YH-18 strain was investigated as a method for producing the bacterial powder.【Method】The conditions of spray drying of YH-18 strain were studied using single-factor and orthogonal tests that were designed to attain a high powder collection rate and number of living bacteria as the outcomes.【Result】The best conditions for the spray drying of B. valeriana YH-18 fermentation liquid are as follows: air inlet temperature, 95 ℃; addition of 15 % skim milk powder; atomization pressure, 0.25 MPa; feeding speed, 10 mL/min; and vacuum degree, -0.03 Pa. Under these conditions, the yield of YH-18-based solid product obtained after the spray drying of B. valeriana YH-18 fermentation liquid reached up to 3.28 × 10 ⁹ cfu/g. The obtained solid fungicide provided potent protection against the disease caused by Phomopsis sp., a pathogen of wood canker disease.【Conclusion】Solid fungicides possess several advantages over liquid fungicides, such as ease of storage and transportation and long shelf life. The spray drying method can be applied in a highly controlled manner and has been shown to impart increased stability to the end product. It is an effective way of converting liquid fungicides to solid fungicides. Therefore, the determination of spray drying conditions of B. valeriana YH-18 fermentation liquid will provide further technical support in the application and production of large-scale solid fungicides in future.

Key words: Bacillus valeriana, microbial solid fungicides, spray drying, process parameters, viable cell counts, collector efficiency

CLC Number:

TQ450.6

WEI Danping, YE Jianren, LIANG Maojin, CHEN Feifei. Spray drying processes of Bacillus valeriana YH-18[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2020, 44(5): 209-214.

Figures/Tables 7

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序号 No.		A/℃		B/%		C/MPa	活菌量/(×10⁹ cfu·g^-1) viable cell counts
1		95		5		0.15	0.108
2		95		10		0.20	0.380
3		95		15		0.25	3.276
4		100		5		0.20	0.356
5		100		10		0.25	0.140
6		100		15		0.15	0.583
7		105		5		0.25	0.029
8		105		10		0.15	0.084
9		105		15		0.20	0.535
K1	3.656		0.494		0.775
K2	1.079		0.604		1.271
K3	0.648		4.393		3.445
K1	1.219		0.165		0.258
K2	0.359		0.201		0.424
K3	0.216		1.464		1.148
极差poor analysis	3.009		3.900		2.670

Spray drying processes of Bacillus valeriana YH-18

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