Induced resistance of Bambusa pervariabilis× Dendrocalamopsis grandis to Arthrinium phaeospermum

doi:10.3969/j.issn.1000-2006.201904037

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2020, Vol. 44 ›› Issue (5): 199-208.doi: 10.3969/j.issn.1000-2006.201904037

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Induced resistance of Bambusa pervariabilis× Dendrocalamopsis grandis to Arthrinium phaeospermum

HE Qianqian¹^,²(), PENG Qi¹, ZONG Xiaozhuo¹, ZHU Tianhui¹, LI Shujiang¹^,³^,^*()

1. College of Forestry, Sichuan Agricultural University, Chengdu 611130, China
2. Yucheng District Forestry Bureau in Ya’an City, Ya’an 625000, China;
3. National Forestry and Grassland Administration Key Laboratory of Forest Resources Conservation and Ecological Safety on the Upper Reaches of the Yangtze River, Chengdu 611130, China

Received:2019-04-17 Revised:2020-04-24 Online:2020-10-30 Published:2020-11-19
Contact: LI Shujiang E-mail:549044997@qq.com;lishujiangsumer@163.com

Abstract

Abstract:

【Objective】 The inactivated protein toxin from Arthrinium phaeospermum(AP-Toxin) was used as the induction factor for the resistance of three Bambusa pervariabilis × Dendrocalamopsis grandis varieties, and the correlations of secretory response with the disease index and resistance were analyzed.【Method】 The upper twigs of healthy one-year-old B. pervariabilis× D. grandis varieties of No. 3, 6, and 8 were inoculated with AP-Toxin by puncturing. The twig samples were collected at four different time points after inoculation. Secretion contents including sugar, proteins, and lipids were determined using the sormy sugar content determination method, biuret method, and alkali droplet method, respectively, with slight modifications. Responses of sugar, protein, and lipid contents after induction with AP-Toxin were assessed.【Result】 After induction with Ap-Toxin, protein, soluble sugar, total sugar, and lipids contents were increased in secretions of all B. pervariabilis × D. grandis varieties. Moreover, the disease index of all varieties increased significantly. The resistance of B. pervariabilis× D. grandis to A. phaeospermum infestation was enhanced. Among the three varieties, the disease index of No.6 was the lowest and its secretory response was the weakest, whereas the disease index of No. 8 was the highest and its secretory response was the strongest. Correlation analyses showed that protein, soluble sugar, total sugar, and lipid contents were significantly and positively correlated with the disease index and resistance, while the reducing sugar content was significantly and negatively correlated with disease resistance.【Conclusion】The resistance of B. pervariabilis × D. grandis varieties to A. phaeospermum could be enhanced using AP-Toxin as the induction factor. The stronger the resistance of the variety, the better the induction effect. Our results lay the theoretical foundation for the application of induced disease resistance for the management of forest pests.

Key words: AP-Toxin, induced factor, Bambusa pervariabilis × Dendrocalamopsis grandis, secretory response, Arthrinium phaeospermum

CLC Number:

S763.11

HE Qianqian, PENG Qi, ZONG Xiaozhuo, ZHU Tianhui, LI Shujiang. Induced resistance of Bambusa pervariabilis× Dendrocalamopsis grandis to Arthrinium phaeospermum[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2020, 44(5): 199-208.

Figures/Tables 8

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Table 1

References 37

[1]	李姝江 . 撑绿杂交竹梢枯病菌蛋白毒素及其精确作用机制研究[D]. 雅安: 四川农业大学, 2013.
	LI S J . Protein toxin produced from Arthrinium phaeospermum causing blight in Bambusa pervariabilis × Dendrocalamopsis grandis and its accurate pathogenic mechanism[D]. Ya’an: Sichuan Agricultural University, 2013.
[2]	朱天辉, 黄宗超, 高强章 , 等. 撑×绿杂交竹梢枯病调查及空间分布型研究[J]. 四川林业科技, 2009,30(1):29-31, 37.
	ZHU T H, HUANG Z C, GAO Q Z , et al. Research of survey and space distribution of Bambusa pervariabilis×Dendrocalamopsis grandis blight[J]. Journal of Sichuan Forestry Science and Technology, 2009,30(1):29-31, 37. DOI: 10.3969/j.issn.1003-5508.2009.01.005.
[3]	LI S J, TANG Y W, FANG X M , et al. Whole-genome sequence of Arthrinium phaeospermum, a globally distributed pathogenic fungus[J]. Genomics, 2020,112(1):919-929. DOI: 10.1016/j.ygeno.2019.06.007. doi: 10.1016/j.ygeno.2019.06.007 pmid: 31175977
[4]	马桂莲, 胡国良, 俞彩珠 , 等. 高节竹梢枯病病原菌及其生物学特性[J]. 浙江林学院学报, 2003,20(1):44-48.
	MA G L, HU G L, YU C Z , et al. Phyllostachys prominens plum shoot wilt pathogenic fungoid and its biological characteristics[J]. Journal of Zhejiang Forestry College, 2003,20(1):44-48. DOI: 10.3969/j.issn.2095-0756.2003.01.011.
[5]	朱天辉, 黄宗超, 高强章 , 等. 撑×绿杂交竹梢枯病病原及发生规律研究[J]. 中国森林病虫, 2009,28(2):10-12.
	ZHU T H, HUANG Z C, GAO Q Z , et al. Study on the pathogen and occurrence regularity of Bambusa pervariabilis × Dendrocalamopsis grandis blight[J]. Forest Pest and Disease, 2009,28(2):10-12. DOI: 1671-0886(2009)02-0010-04.
[6]	杨佐忠, 叶建仁 . 杂交竹枯梢病的病原鉴定[J]. 四川农业大学学报, 2004,22(3):225-227.
	YANG Z Z, YE J R . Identification on pathogen of hybrid bamboo blight[J]. Journal of Sichuan Agricultural University, 2004,22(3):225-227. DOI: 10.3969/j.issn.1000-2650.2004.03.007.
[7]	李姝江, 朱天辉, 李芳莲 , 等. 撑绿杂交竹梢枯病菌拮抗细菌筛选及其抗菌物质研究[J]. 中国生物防治, 2010,26(S1):55-61.
	LI S J, ZHU T H, LI F L, et al. Selection of antagonistic bacterium against Arthrinium phaeospermum and analysis of its antibacterial substance[J]. Chinese Journal of Biological Control, 2010,26(S1):55-61. DOI: 1005-9261(2010)增刊-0055-07.
[8]	李姝江, 梁漫, 朱天辉 , 等. 撑绿杂交竹梢枯病拮抗菌的筛选及抗菌蛋白分析[J]. 南京林业大学学报(自然科学版), 2013,37(6):27-32.
	LI S J, LIANG M, ZHU T H , et al. Selection of an antagonistic bacterium against Arthrinium phaeospermum and its antibacterial protein analysis[J]. J Nanjing For Uni (Nat Sci Ed), 2013,37(6):27-32. DOI: 10.3969/j.issn.1000-2006.2013.06.006.
[9]	黄宗超, 朱天辉 . 撑绿杂交竹枯梢病菌的室内杀菌剂筛选[J]. 中国森林病虫, 2007,26(3):35-38.
	HUANG Z C, ZHU T H . Effective fungicide screening against pathogen of hybrid bamboo blight in lab[J]. Forest Pest and Disease, 2007,26(3):35-38. DOI: 10.3969/j.issn.1671-0886.2007.03.011.
[10]	LI S J, ZHU T H . Purification of the toxin protein Pc from Arthrinium phaeospermum and its effect on the defence enzymes of Bambusa pervariabilis × Dendrocalamopsis grandis varieties[J]. Forest Pathology, 2014,44(2):96-106. DOI: 10.1111/efp.12072. doi: 10.1111/efp.12072
[11]	LI S J, ZHU T H, ZHU H M Y , et al. Purification of protein AP-toxin from Arthrinium phaeospermum causing blight in Bambusa pervariabilis × Dendrocalamopsis grandis and its metabolic effects on four bamboo varieties[J]. Phytopathology, 2013,103(2):135-145. DOI: 10.1094/phyto-07-12-0164-r. doi: 10.1094/PHYTO-07-12-0164-R pmid: 23095467
[12]	KELLER N P, BUTCHKO R, SARR A , et al. A visual pattern of mycotoxin production in maize kernels by Aspergillus spp.[J]. Phytopathology, 1994,84(5):483-488. DOI: 10.1094/Phyto-84-483. doi: 10.1094/Phyto-84-483
[13]	SHAHIN E A, SPIVEY R . A single dominant gene for Fusarium wilt resistance in protoplast-derived tomato plants[J]. Theoretical and Applied Genetics, 1986,73(2):164-169. DOI: 10.1007/bf00289270. doi: 10.1007/BF00289270 pmid: 24240846
[14]	EGUSA M, AKAMATSU H, TAKASHI T , et al. Induced resistance in tomato plants to the toxin-dependent necrotrophic pathogen Alternaria alternate[J]. Physiological and Molecular Plant Pathology, 2008,73(4-5):67-77. DOI: 10.1016/j.pmpp.2009.02.001.
[15]	杨艳丽, 肖浪涛, 胡先奇 . 马铃薯晚疫病菌毒素与寄主品种抗性关系研究[J]. 中国农业科学, 2009,42(6):2202-2210.
	YANG Y L, XIAO L T, HU X Q . Study on the relationship between the toxin of Phytophthora infestans (Mont.) de bary and resistance of potato[J]. Scientia Agricultural Sinica, 2009,42(6):2202-2210. DOI: 10.3864/j.issn.0578-1752.2009.06.042.
[16]	何倩倩, 刘雨欣, 方馨玫 , 等. 撑绿杂交竹抗梢枯病诱导因子筛选及其持续期研究[J]. 植物保护, 2019,45(2):81-89.
	HE Q Q, LIU Y X, FANG X M , et al. Screening of inducing factors of Bambusa pervariabilis×Dendrocalamopsis grandis against Arthrinium phaeospermum and their duration[J]. Plant Protection, 2019,45(2):81-89. DOI: 10.16688/j.zwbh.2018053.
[17]	LI S J, ZHU T H . Biochemical response and induced resistance against anthracnose (Colletotrichum camelliae) of camellia (Camellia pitardii) by chitosan oligosaccharide application[J]. Forest Pathology, 2013,43:67-76. DOI: 10.1111/j.1439-0329.2012.00797.x.
[18]	方中达 . 植病研究方法[M]. 3版.北京: 中国农业出版社, 1998: 8-12.
	FANG Z D. Method of plant disease research[M]. 3rd ed. Beijing: China Agricultural Press, 1988: 8-12.
[19]	王俊刚, 张树珍, 杨本鹏 , 等. 3,5-二硝基水杨酸(DNS)法测定甘蔗茎节总糖和还原糖含量[J]. 甘蔗糖业, 2008,27(5):45-48.
	WANG J G, ZHANG S Z, YANG B P , et al. Application of 3,5-dinitrosalicylic acid (DNS) method to test the reducing sugar and water-soluble total sugar content in sugarcane internodes[J]. Sugarcane and Canesugar, 2008,27(5):45-48. DOI: 10.3969/j.issn.1005-9695.2008.05.010.
[20]	钟岩, 潘浦群, 王艳红 , 等. 苯酚-硫酸法测定鲜人参中多糖含量[J]. 时珍国医国药, 2008,19(8):1957-1958.
	ZHONG Y, PAN P Q, WANG Y H , et al. Determination of the content of polisaccharides in fresh root of Panax ginseng by the method of phenol vitril[J]. Shizhen Medicine and Materia Medica Research, 2008,19(8):1957-1958. DOI: 10.3969/j.issn.1008-0805.2008.08.079.
[21]	王晓丽, 苟琳 . 生物化学实验教程 [M]. 成都: 四川科学技术出版社, 2005: 53-54, 63, 72-73.
	WANG X L, GOU L. Biochemistry experiment course [M]. Chengdu: Sichuan Science and Technology Press, 2005: 53-54, 63, 72-73.
[22]	BEDBROOK J R MATTEWS R E F . Changes in the flow of early products of photosynthetic carbon fixation associated with the replication of TYMV[J]. Virology, 1973,53(1):84-91. DOI: 10.1016/0042-6822(73)90467-4. doi: 10.1016/0042-6822(73)90467-4 pmid: 4706713
[23]	杨莉 . 暗孢节菱孢菌对撑×绿撑绿杂交竹致病机理的研究[D]. 雅安: 四川农业大学, 2009.
	YANG L . The dissection on pathogenic mechanism of Arthrinium phaeospermum to Bambusa pervariabilis × Dendrocalamopsis grandis hybrid bamboo[D]. Ya’an: Sichuan Agricultural University, 2009.
[24]	李姝江, 朱天辉, 杨莉 , 等. 暗孢节菱孢菌非蛋白类毒素对撑绿杂交竹生理代谢的影响[J]. 植物病理学报, 2011,41(6):587-595.
	LI S J, ZHU T H, YANG L , et al. Effects of nonprotein toxin from Arthrinium phaeospermum on physiological metabolism of Bambusa pervariabilis × Dendrocalamopsis grandis hybrid bamboo[J]. Acta Phytopathologica Sinica, 2011,41(6):587-595. DOI: 0412-0914(2011)06-0587-09.
[25]	景康康 . 牛角花齿蓟马(Odontothrips loti)为害对苜蓿同化产物及矿质元素分配的影响[D]. 兰州: 甘肃农业大学, 2013.
	JING K K . Effect of phptosynthate and mineral elements distribution in alfalfa damaged by Odontothrips loti[D]. Lanzhou: Gansu Agricultural University, 2013.
[26]	敖雪, 邵世勤, 张少英 , 等. 甜菜抗(感)丛根病品种苗期某些生理生化特性差异的研究[J]. 中国甜菜糖业, 2005, ( 1):15-17.
	AO X, SHAO S Q, ZHANG S Y , et al. Study on the difference of some physiological and biochemical characters between resistance and susceptible to rhizomania in sugar beet varieties in seedling stage[J]. China Beet & Sugar, 2005, ( 1):15-17. DOI: 10.3969/j.issn.1002-0551.2005.01.004.
[27]	ASHRY N A, MOHAMED H I . Impact of secondary metabolites and related enzymes in flax resistance and or susceptibility to powdery mildew[J]. African Journal of Biotechnology, 2012,11(5):78-85. DOI: 10.5897/AJB11.1023.
[28]	ASHFAD M, KHAN M A, JAVED N , et al. Effect of urdbean leaf crinkle virus infection on total soluble protein and antioxidant enzymes in blackgram plants[J]. Pakistan Journal of Botany, 2010,42(1):447-454. DOI: 10.1127/1438-9134/2010/0136-0325.
[29]	程洁 . 白菜、甘蓝根肿病相关生理生化指标的分析[D]. 重庆: 西南大学, 2013.
	CHENG J . Study for the related analysis of physiological and biochemical mechanism in Chinese cabbage and cabbage[D]. Chongqing: Southwest University, 2013.
[30]	MULLET J E, KLEIN P G, KLEIN R R . Chlorophyll regulates accumulation of the plastid-encoded chlorophyll apoproteins CP43 and D1 by increasing apoprotein stability[J]. Proceedings of The National Academy of Sciences, 1990,87:4038-4042. DOI: 10.1073/pnas.87.11.4038. doi: 10.1073/pnas.87.11.4038
[31]	王金生, 张学君, 方中达 . 马铃薯品种对细菌软腐病抗性生理指标的研究Ⅱ.品种还原糖含量及其与软腐病抗性的关系[J]. 南京农业大学学报, 1990,13(2):69-75.
	WANG J S, ZHANG X J, FANG Z D . Physiology of resistance of potato varieties to soft rot disease II. Relationship between the content of reducing sugars in tubers and their resistance[J]. Journal of Nanjing Agricultural University, 1990,13(2):69-75. DOI: 10.7685/j.issn.1000-2030.1990.02.013.
[32]	龙书生, 李亚玲, 张宇宏 , 等. 糖分含量作为抗镰刀菌茎腐病玉米品种的育种指标研究[J]. 山东农业大学学报(自然科学版), 1999,30(4):372-376, 380.
	LONG S S, LI Y L, ZHANG Y H , et al. Sugar levels as a breeding index of variety resistant to corn stalk rot[J]. Journal of Shandong Agricultural University, 1999,30(4):372-376, 380. DOI: 10.3969/j.issn.1000-2324.1999.04.011.
[33]	丁九敏, 高洪斌, 刘玉石 , 等. 黄瓜霜霉病抗性与叶片中生理生化物质含量关系的研究[J]. 辽宁农业科学, 2005,5(1):11-13.
	DING J M, GAO H B, LIU Y S , et al. Study on the relationship between resistance to cucumber downy mildew and the content of physiological-biochemical matter in the leaves[J]. Liaoning Agricultural Sciences, 2005,5(1):11-13. DOI: 10.3969/j.issn.1002-1728.2005.01.004.
[34]	周博如, 李永镐, 刘太国 , 等. 不同抗性的大豆品种接种大豆细菌性疫病菌后可溶性蛋白、总糖含量变化的研究[J]. 大豆科学, 2000,19(2):111-114.
	ZHOU B R, LI Y H, LIU T G , et al. Study on the changes of soluble protein and total sugar in the first leaves of soybean varieties inoculated with Pseudomonas syringes pv. glycinea[J]. Soybean Science, 2000,19(2):111-114. DOI: 10.1007/s11769-000-0013-x.
[35]	FAHY E, SUBRAMANIAM S, MURPHY R C , et al. Update of the LIPID MAPS comprehensive classification system for lipids[J]. Journal of Lipid Research, 2009,50(Suppl):S9-S14. DOI: 10.1194/jlr.R800095-JLR200. doi: 10.1194/jlr.R800095-JLR200
[36]	SUBRAMANIAM S, FAHY E, GUPTA S , et al. Bioinformatics and systems biology of the lipidome[J]. Chemical Reviews, 2011,111(10):6452-6490. DOI: 10.1021/cr200295k. doi: 10.1021/cr200295k pmid: 21939287
[37]	ROTH L E, STACEY G . Cytoplasmic membrane systems involved in bacterium release into soybean nodule cells as studied with two Bradyrhizobium japonicum mutant strains[J]. European Journal of Cell Biology, 1989,49(1):24-32. pmid: 2759102

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项目 item	蛋白质含量 protein content	可溶性多糖含量 soluble sugar content	还原糖含量 reducing sugar content	总糖含量 total sugar content	脂类含量 lipid content
时间time	0.510	0.318	0.054	0.469	-0.008
抗性resistance	0.989^*	0.992^*	-0.998^*	0.997^*	0.993^*
病情指数disease index	-0.924^*	-0.853^*	0.907^*	-0.814^*	-0.863^*

Induced resistance of Bambusa pervariabilis× Dendrocalamopsis grandis to Arthrinium phaeospermum

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