Effects of arbuscular mycorrhiza fungi on the growth and physiological metabolism of Pyrus betulaefolia Bunge seedlings under saline-alkaline stress

doi:10.3969/j.issn.1000-2006.202001045

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2020, Vol. 44 ›› Issue (6): 152-160.doi: 10.3969/j.issn.1000-2006.202001045

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Effects of arbuscular mycorrhiza fungi on the growth and physiological metabolism of Pyrus betulaefolia Bunge seedlings under saline-alkaline stress

JIANG Lei¹^,²(), LI Huanyong³, ZHANG Qin⁴, ZHANG Huilong¹, QIAO Yanhui⁵, ZHANG Huaxin¹, YANG Xiuyan¹^,^*()

1. Research Center of Saline and Alkali Land of National Forestry and Grassland Administration, Chinese Academy of Forestry, Beijing 100091, China
2. Langfang Academy of Agriculture and Forestry, Langfang 065000, China
3. Tianjin Institute of Forestry and Pomology, Tianjin 300384, China
4. Zhangjiagang Yinghua Material Technology Co., Ltd., Zhangjiagan 215600,China
5. Shandong Academy of Forestry Science, Jinan 250014,China

Received:2020-01-22 Revised:2020-03-05 Online:2020-11-30 Published:2020-12-07
Contact: YANG Xiuyan E-mail:690826912@qq.com;zsueyxy@126.com

Abstract

Abstract:

【Objective】To provide a scientific guidance for the application of Pyrus betulaefolia Bunge rhizome seedlings in afforestation of saline-alkaline lands, the effects of arbuscular mycorrhizal fungi (AMF) on plant growth and physiological characteristics in P. betulaefolia Bunge seedlings under saline-alkaline stress were investigated. 【Method】A two-factor experimental design was conducted to compare the impacts of AMF inoculation and saline-alkaline stress on the growth and physiological metabolism on one-year-old P. betulaefolia Bunge seedlings. The seedlings were divided into two groups [AMF (Funneliformis mosseae) inoculated and non-inoculated] and were treated with Na₂CO₃ (concentration gradient: 0, 100, 200 and 300 mmol/L) at the same time, and their growth and physiological characteristics were measured. 【Result】The AMF significantly increased plant height growth and biomass accumulation of P. betulaefolia Bunge seedlings under the Na₂CO₃ treatment. The decrease of △H and biomass under the salt-alkali stress was inhibited by AMF inoculation. AMF inoculation significantly affected the absorption and distribution of K⁺, Na⁺,Ca²⁺ and Mg²⁺ in P. betulaefolia Bunge. Na⁺ content in seedling roots of the inoculated was 10.8%, 21.6% and 19.4 lower than that of the non-inoculated under 3 different Na₂CO₃ concentrations, respectively. The K⁺/Na⁺, Ca²⁺/Na⁺ and Mg²⁺/Na⁺ ratios in the roots and leaves of seedlings inoculated with AMF were higher than those of the seedlings without inoculation because of the decline of Na⁺ absorption. Inoculation with AMF can reduce proline and malondialdehyde (MDA) content and increase the chlorophyll contents in seedling leaves, indicating that AMF can alleviate the oxidative damage and ion toxicity of saline-alkali stress on seedlings. 【Conclusion】It is conclude that AMF inoculation increased the content of photosynthetic pigment, maintained the relative ions balance in the plant and alleviate the oxidative stress, thus improving the salt-alkali tolerance and promoting the seedlings growth of P. betulaefolia Bunge under saline-alkaline stress.

Key words: saline-alkaline stress, Pyrus betulaefolia Bunge, arbuscular mycorrhiza fungi (AMF), ion homeostasis, salt-alkali tolerance

CLC Number:

S728

JIANG Lei, LI Huanyong, ZHANG Qin, ZHANG Huilong, QIAO Yanhui, ZHANG Huaxin, YANG Xiuyan. Effects of arbuscular mycorrhiza fungi on the growth and physiological metabolism of Pyrus betulaefolia Bunge seedlings under saline-alkaline stress[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2020, 44(6): 152-160.

Figures/Tables 4

Fig.1

Fig.2

Table 1

Effects of AMF on inorganic ion concentrations in different organs of P. betulaefolia Bunge seedling under salt-alkali stress"

Na₂CO₃ 浓度 /(mmol·L^-1) Na₂CO₃ concentration	菌根 mycorr- hiza	K⁺/%					Na⁺/%						K⁺/Na⁺						Ca²⁺/%
Na₂CO₃ 浓度 /(mmol·L^-1) Na₂CO₃ concentration	菌根 mycorr- hiza	根 root	茎 stem		叶 leaf		根 root		茎 stem		叶 leaf		根 root		茎 stem		叶 leaf		根 root	茎 stem
CK	+AM	0.526± 0.004 a	0.659± 0.003 a		1.280± 0.001 d		0.827± 0.011 c		0.855± 0.003 d		1.015± 0.003 e		0.636± 0.012 a		0.771± 0.007 cd		1.261± 0.003 c		0.227± 0.003 e	0.843± 0.001 c
CK	-AM	0.450± 0.004 b	0.591± 0.001 d		1.250± 0.003 e		0.844± 0.023 c		0.753± 0.002 f		1.010± 0.001 e		0.533± 0.011 b		0.785± 0.003 c		1.238± 0.002 b		0.312± 0.001 a	0.978± 0.002 b
100	+AM	0.379± 0.006 c	0.630± 0.003 b		1.368± 0.004 b		0.847± 0.002 c		0.979± 0.000 b		0.921± 0.001 f		0.447± 0.006 c		0.643± 0.004 e		1.485± 0.006 a		0.235± 0.001 d	0.798± 0.004 d
100	-AM	0.306± 0.003 d	0.593± 0.002 d		1.637± 0.004 a		0.950± 0.001 a		0.682± 0.001 g		1.334± 0.008 c		0.322± 0.004 e		0.870± 0.004 b		1.227± 0.005 c		0.268± 0.002 b	1.019± 0.009 a
200	+AM	0.294± 0.003 e	0.609± 0.004 c		1.211± 0.004 f		0.741± 0.006 d		0.685± 0.001 g		1.019± 0.001 e		0.397± 0.004 d		0.889± 0.008 a		1.188± 0.004 d		0.210± 0.001 f	0.789± 0.006 d
200	-AM	0.298± 0.003d e	0.597± 0.004 d		1.291± 0.002 c		0.945± 0.002 a		0.961± 0.003 c		1.283± 0.003 d		0.315± 0.004 e		0.621± 0.007 f		1.006± 0.002 e		0.229± 0.001 c	0.800± 0.002 d
300	+AM	0.195± 0.002 g	0.463± 0.004 e		1.024± 0.003 h		0.731± 0.005 d		1.205± 0.003 a		1.500± 0.001 b		0.267± 0.001 f		0.384± 0.002 g		0.683± 0.002 f		0.212± 0.002 de	0.520± 0.002 e
300	-AM	0.221± 0.003 f	0.591± 0.002 d		1.063± 0.002 g		0.907± 0.001 b		0.771± 0.001 e		1.696± 0.002 a		0.244± 0.003 g		0.767± 0.001 d		0.626± 0.001 g		0.221± 0.004 f	0.798± 0.002 d
接种AMF AMF		**	NS		NS		**		**		**		**		**		**		**	**
盐碱处理 Na₂CO₃		**	**		**		**		**		**		**		**		**		**	**
AMF×Na₂CO₃		**	**		**		**		*		**		**		*		**		**	**
Na₂CO₃ 浓度 /(mmol·L^-1) Na₂CO₃ concentration	菌根 mycorr- hiza	Ca²⁺/%		Ca²⁺/Na⁺						Mg²⁺/%						Mg²⁺/Na⁺
Na₂CO₃ 浓度 /(mmol·L^-1) Na₂CO₃ concentration	菌根 mycorr- hiza	叶 leaf		根 root		茎 stem		叶 leaf		根 root		茎 stem		叶 leaf		根 root		茎 stem		叶 leaf
CK	+AM	0.692± 0.001 c		0.275± 0.006 d		0.985± 0.005 e		0.681± 0.001 b		0.093± 0.001 bc		0.105± 0.004 b		0.157± 0.001 ab		0.113± 0.003 ab		0.120± 0.034 c		0.154± 0.000 bc
CK	-AM	0.737± 0.004 b		0.370± 0.011 a		1.300± 0.003 b		0.730± 0.004 a		0.105± 0.009 a		0.114± 0.002 a		0.163± 0.003 a		0.124± 0.011 a		0.151± 0.003 b		0.162± 0.003 a
100	+AM	0.601± 0.002 e		0.278± 0.001 c		0.815± 0.004 f		0.652± 0.001 c		0.090± 0.001 bc		0.123± 0.001 bc		0.144± 0.002 d		0.106± 0.001 bc		0.105± 0.001 d		0.156± 0.002 ab
100	-AM	0.684± 0.001 c		0.282± 0.002 c		1.500± 0.016 a		0.512± 0.003 f		0.100± 0.001 ab		0.114± 0.002 a		0.140± 0.003 d		0.105± 0.001 bc		0.167± 0.002 a		0.105± 0.001 e
200	+AM	0.638± 0.002 d		0.283± 0.001 c		1.151± 0.010 c		0.627± 0.002 d		0.084± 0.001 cd		0.100± 0.003 c		0.145± 0.002 cd		0.114± 0.002 ab		1.012± 0.043 b		0.142± 0.002 c
200	-AM	0.755± 0.003 a		0.258± 0.002 d		0.832± 0.002 f		0.588± 0.001 e		0.095± 0.002 abc		0.103± 0.002 bc		0.152± 0.001 bc		0.100± 0.002 c		0.107± 0.002 d		0.118± 0.001 d
300	+AM	0.938± 0.024 a		0.313± 0.002 b		0.431± 0.001 g		0.625± 0.016 d		0.075± 0.006 d		0.083± 0.006 e		0.132± 0.002 e		0.103± 0.001 c		0.069± 0.005 e		0.088± 0.001 f
300	-AM	0.692± 0.003 c		0.234± 0.005 e		1.034± 0.001 d		0.408± 0.002 g		0.092± 0.002 bc		0.092± 0.001 d		0.139± 0.004 de		0.101± 0.002 c		0.119± 0.001 c		0.082± 0.002 f
接种AMF AMF		NS		NS		**		**		**		*		*		*		**		NS
盐碱处理 Na₂CO₃		**		**		NS		NS		**		*		**		**		**		**
AMF×Na₂CO₃		**		**		**		**		**		NS		NS		NS		NS		**

Table 1

Table 2

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Na₂CO₃浓度 Na₂CO₃ concentration	菌根 mycorrhiza	含量
Na₂CO₃浓度 Na₂CO₃ concentration	菌根 mycorrhiza	叶绿素a/ (mg·g^-1) Chl a/	叶绿素b (mg·g^-1) Chl b/	总叶绿素/ (mg·g^-1 ) Chl a+b	类胡罗卜素/ (mg·g^-1) Car	可溶性糖/ (mmol·g^-1) SS	脯氨酸/ (μg·g^-1) Pro	丙二醛/ (nmol·g^-1) MDA	还原型谷胱甘肽/ (μmol·g^-1) GSH
CK	+AM	0.809±0.008 a	0.382±0.012 a	1.191±0.005 a	0.140±0.006 abc	0.078±0.002 c	11.829±0.754 b	1.001±0.000 d	0.659±0.013 b
CK	-AM	0.649±0.052 bc	0.266±0.014 cd	0.916±0.067 bc	0.130±0.010 abc	0.087±0.002 bc	10.281±1.939 b	1.533±0.000 bcd	1.002±0.164 ab
100	+AM	0.747±0.034 ab	0.353±0.039 ab	1.100±0.072 ab	0.131±0.003 abc	0.083±0.004 bc	12.052±1.991 b	1.234±0.000 d	0.842±0.081 b
100	-AM	0.739±0.046 ab	0.296±0.016 c	1.035±0.062 ab	0.153±0.010 a	0.092±0.004 bc	8.611±1.832 b	1.567±0.000 cd	1.086±0.010 a
200	+AM	0.802±0.034 a	0.355±0.009 ab	1.157±0.043 a	0.148±0.005 ab	0.094±0.006 b	15.340±1.251 b	1.333±0.000 cd	0.750±0.072 b
200	-AM	0.586±0.023 c	0.238±0.013 cd	0.824±0.036 c	0.121±0.003 c	0.093±0.009 bc	20.982±5.877 a	2.000±0.000 bc	1.088±0.133 a
300	+AM	0.538±0.051 c	0.213±0.021 d	0.751±0.072 c	0.126±0.005 bc	0.096±0.004 b	21.954±1.036 a	2.300±0.000 b	1.146±0.058 a
300	-AM	0.553±0.066 c	0.214±0.026 d	0.768±0.092 c	0.126±0.013 bc	0.124±0.003 a	23.833±3.253 a	2.967±0.000 a	1.158±0.025 a
接种AMF inoculated AMF		NS	**	*	NS	NS	NS	**	*
盐碱处理 Na₂CO₃		**	*	**	NS	*	**	**	N.S
AMF×Na₂CO₃		*	*	*.	NS	*	NS	NS	NS

Effects of arbuscular mycorrhiza fungi on the growth and physiological metabolism of Pyrus betulaefolia Bunge seedlings under saline-alkaline stress

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