Application of CRISPR/Cas technique in woody plant improvement

doi:10.12302/j.issn.1000-2006.202010017

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2021, Vol. 45 ›› Issue (6): 24-30.doi: 10.12302/j.issn.1000-2006.202010017

Special Issue: 专题报道；林木 CRISPR/Cas基因编辑专题

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Application of CRISPR/Cas technique in woody plant improvement

HOU Jing(), MAO Jinyan, ZHAI Hui, WANG Jie, YIN Tongming^*()

Key Laboratory of Forest Genetics and Biotechnology of Ministry of Education, Co-Innovation Center for the Sustainable Forestry in Southern China, College of Forestry,Nanjing Forestry University,Nanjing 210037, China

Received:2020-10-12 Accepted:2021-02-27 Online:2021-11-30 Published:2021-12-02
Contact: YIN Tongming E-mail:jinghou@njfu.edu.cn;tmyin@njfu.com.cn

Abstract

Abstract:

Long breeding periods and scarce germplasm resources have become the main bottlenecks in woody plant improvement. Recently, the CRISPR/Cas system has been developed into a precision site-directed editing technology that has been widely used in the breeding of woody plants such as Populus spp., Malus spp., Citrus spp., Vitis vinifera and Cassava spp.. The yield, quality, biotic- and abiotic-stress resistance, and other traits of woody plants have been significantly improved by genome editing, achieving the fixation of excellent genotypes within a single generation. This approach has accelerated the breeding process of woody plants and improves their breeding efficiency. Here, we summarize the application of CRISPR/Cas technology to woody plants, analyzing existing problems and future development trends. This review aims to provide a useful reference for gene function research and improvement of woody plants.

Key words: CRISPR/Cas, genome editing, molecular breeding, woody plants, breed improvement

CLC Number:

Q943.2

HOU Jing, MAO Jinyan, ZHAI Hui, WANG Jie, YIN Tongming. Application of CRISPR/Cas technique in woody plant improvement[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2021, 45(6): 24-30.

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Application of CRISPR/Cas technique in woody plant improvement

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