Effects of elevated CO2 on photosynthetic characteristics and monoterpene emissions in Pinus massoniana seedlings

doi:10.3969/j.issn.1000-2006.201903034

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2020, Vol. 44 ›› Issue (6): 71-78.doi: 10.3969/j.issn.1000-2006.201903034

Previous Articles Next Articles

Effects of elevated CO₂ on photosynthetic characteristics and monoterpene emissions in Pinus massoniana seedlings

YE Siyuan(), SHANG He^*(), CHEN Zhan, CAO Jixin

Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, Key Laboratory of Forest Ecology and Environment, Natronal Forestry and Grassland Administration, Beijing 100091, China

Received:2019-03-13 Revised:2019-06-05 Online:2020-11-30 Published:2020-12-07
Contact: SHANG He E-mail:ysy1994@126.com;shanghechina@126.com

Abstract

Abstract:

【Objective】Biogenic volatile organic compounds(BVOCs)are the main components of volatile organic compounds (VOCs) in the atmosphere and one of the key compounds of vegetation that affect the environment and climate. With the intensification of the global climate change, the research on BVOCs emission by plants under increasing CO₂ levels has gained a considerable interest. Effects of the elevated CO₂ concentrations on photosynthetic characteristics and monoterpene emission rate in Masson pine (Pinus massoniana) seedlings were studied to improve the understanding of the response mechanism of plant BVOC emissions to the climate change.【Method】According to the atmospheric CO₂ concentrations in different carbon emission scenarios reported in the Intergovernmental Panel on Climate Change (IPCC) fifth assessment report, 1-year-old Masson pine seedlings were fumigated with CO₂ concentration gradients, consisting of ambient air (437 μmol/mol,CK), 550 μmol/mol, 750 μmol/mol, and 1 000 μmol/mol in 12 open-top chambers (three replicated chambers for each), from September 10th to November 20th, 2017. The elevated CO₂ concentrations were maintained for 9 hours per day from 08:00 to 17:00. In the middle of October and November, the net photosynthetic rate and stomatal conductance of Masson pine seedlings were measured in ambient air from 09:00 to 11:30. Pine needles were collected for a chlorophyll determination. Monoterpene emissions of Masson pine seedlings were also sampled in the middle of October and November using a dynamic headspace adsorption sampling method with 200 mL/min for 30 min; all sampling experiments were carried out during 09:00-11:30 and 13:30-15:30. Monoterpene samples were collected through Tenax-TA glass tubes (Mesh 60/80) and analyzed using TDS-GC/MS. 【Result】Chlorophyll a and chlorophyll b of the plant seedlings exposed to 750 μmol/mol and 1 000 μmol/mol CO₂ for 60 days were both significantly lower than those under CK, and the total chlorophyll content decreased by 17.54% and 29.82%, respectively. However, the difference was not significant when fumigation was performed for 30 days. When measured in ambient air, the net photosynthetic rate and stomatal conductance of Masson pine seedlings treated with 60-day CO₂ enrichment was significantly lower than those under CK; however, there was also no significant difference when treated for 30 days. In addition, the total chlorophyll content of Masson pine seedlings was positively correlated with the leaf net photosynthetic rate significantly. Monoterpene emissions by Pinus massoniana were decreased by the elevated CO₂ after 60-day fumigation; the emission rate reached the lowest at the concentration of 1 000 μmol/mol, with a decrease of 19.43% (fumigated for 30 days) and 59.62% (fumigated for 60 days). After 60-day treatment, the monoterpene emission rate of P. massoniana seedlings was significantly and positively correlated to the leaf net photosynthetic rate and stomatal conductance.【Conclusion】With the extension of fumigation time,the photosynthetic acclimation of P. massoniana was revealed; the decline in monoterpene emissions may be attributed to the decrease in stomatal conductance under the condition of elevated CO₂ concentration.

Key words: CO₂ concentration, Pinus massoniana, net photosynthetic rate, stomatal conductance, monoterpene emission rate, Open-Top Chamber (OTCs)

CLC Number:

S718.5

YE Siyuan, SHANG He, CHEN Zhan, CAO Jixin. Effects of elevated CO₂ on photosynthetic characteristics and monoterpene emissions in Pinus massoniana seedlings[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2020, 44(6): 71-78.

Figures/Tables 4

Table 1

Fig.1

Fig.2

Fig.3

References 44

[1]	WMO Greenhouse Gas Bulletin. The state of greenhouse gases in the atmosphere based on global observations through 2017 [R]. Genewa:World Meteorological Organization, 2018.
[2]	IPCC. Climate Change 2014: Synreport report. Contribution of working groups Ⅰ, Ⅱ and Ⅲ to the fifth assessment Report of the intergovernmental panel on climate change[R]. Geneva, Switzerland: IPCC, 2014.
[3]	张振花, 孙胜, 刘洋, 等. 增施CO₂对温室番茄结果期叶片光合特性的影响[J]. 生态学杂志, 2018,37(5):1398-1402.
	ZHANG Z H, SUN S, LIU Y, et al. Effects of CO₂ enrichment on photosynthetic characteristics of greenhouse tomato during fruiting stage[J]. Chinese Journal of Ecology, 2018,37(5):1398-1402. DOI: 10.13292/j.1000-4890.201805.010.
[4]	毛子军, 赵溪竹, 刘林馨, 等. 3种落叶松幼苗对CO₂升高的光合生理响应[J]. 生态学报, 2010,30(2):317-323.
	MAO Z J, ZHAO X Z, LIU L X, et al. Photosynthetic physiological characteristics in response to elevated CO₂ concentration of three larch (Larix) species seedlings[J]. Acta Ecologica Sinica, 2010,30(2):317-323.
[5]	汤文华, 窦全琴, 潘平平, 等. 不同薄壳山核桃品种光合特性研究[J]. 南京林业大学学报(自然科学版), 2020,44(3):81-88.
	TANG W H, DOU Q Q, PAN P P, et al. Photosynthetic characteristics of grafted plants of different Carya illinoinensis varieties[J]. J Nanjing For Univ(Nat Sci Ed), 2020,44(3):81-88. DOI: 10.3969/j.issn.1000-2006.201903004.
[6]	郑凤英, 彭少麟. 植物生理生态指标对大气CO₂浓度倍增响应的整合分析[J]. 植物学报, 2001,43(11):1101-1109.
	ZHENG F Y, PENG S L. Meta-analysis of the response of plant ecophysiological variables to doubled atmospheric CO₂ concentrations[J]. Acta Botanica Sinica, 2001,43(11):1101-1109. DOI: 10.3321/j.issn:1672-9072.2001.11.001.
[7]	GAMAGE D, THOMPSON M, SUTHERLAND M, et al. New insights into the cellular mechanisms of plant growth at elevated atmospheric carbon dioxide concentrations[J]. Plant, Cell & Environment, 2018,41(6):1233-1246. DOI: 10.1111/pce.13206
[8]	PEÑUELAS J, LLUSIÀ J. Plant VOC emissions: making use of the unavoidable[J]. Trends in Ecology & Evolution, 2004,19(8):402-404. DOI: 10.1016/j.tree.2004.06.002.
[9]	THEIS N, LERDAU M. The evolution of function in plant secondary metabolites[J]. International Journal of Plant Sciences, 2003,164(S3):S93-S102. DOI: 10.1086/374190.
[10]	PEÑUELAS J, STAUDT M. BVOCs and global change[J]. Trends in Plant Science, 2010,15(3):133-144. DOI: 10.1016/j.tplants.2009.12.005.
[11]	PACIFICO F, HARRISON S P, JONES C D, et al. Isoprene emissions and climate[J]. Atmospheric Environment, 2009,43(39):6121-6135. DOI: 10.1016/j.atmosenv.2009.09.002.
[12]	HALLQUIST M, WENGER J C, BALTENSPERGER U, et al. The formation, properties and impact of secondary organic aerosol: current and emerging issues[J]. Atmospheric Chemistry and Physics, 2009,9(14):5155-5236. DOI: 10.5194/acp-9-5155-2009.
[13]	GUENTHER A, HEWITT C N, ERICKSON D, et al. A global model of natural volatile organic compound emissions[J]. Journal of Geophysical Research, 1995,100(D5):8873-8892. DOI: 10.1029/94jd02950.
[14]	HEYWORTH C J, IASON G R, TEMPERTON V, et al. The effect of elevated CO₂ concentration and nutrient supply on carbon-based plant secondary metabolites in Pinus sylvestris L[J]. Oecologia, 1998,115(3):344-350. DOI: 10.1007/s004420050526.
[15]	STAUDT M, JOFFRE R, RAMBAL S, et al. Effect of elevated CO₂ on monoterpene emission of young Quercus ilex trees and its relation to structural and ecophysiological parameters[J]. Tree Physiology, 2001,21(7):437-445. DOI: 10.1093/treephys/21.7.437.
[16]	LORETO F, FISCHBACH R J, SCHNITZLER J P, et al. Monoterpene emission and monoterpene synthase activities in the Mediterranean evergreen oak Quercus ilex L. grown at elevated CO₂ concentrations[J]. Global Change Biology, 2001,7(6):709-717. DOI: 10.1046/j.1354-1013.2001.00442.x.
[17]	花圣卓, 陈俊刚, 余新晓, 等. 温带典型森林树种的萜烯类化合物排放及其与环境要素的相关性[J]. 林业科学, 2016,52(11):19-28.
	HUA S Z, CHEN J G, YU X X, et al. Correlation between terpenes emission from typical forest tree species and environmental elements in temperate zone[J]. Scientia Silvae Sinicae, 2016,52(11):19-28. DOI: 10.11707/j.1001-7488.20161103.
[18]	王志辉, 张树宇, 陆思华, 等. 北京地区植物VOCs排放速率的测定[J]. 环境科学, 2003,24(2):7-12.
	WANG Z H, ZHANG S Y, LU S H, et al. Screenings of 23 plant species in Beijing for volatile organic compound emissions[J]. Chinese Journal of Environmental Science, 2003,24(2):7-12. DOI: 10.13227/j.hjkx.2003.02.002.
[19]	陈颖, 史奕, 何兴元. 沈阳市四种乔木树种BVOCs排放特征[J]. 生态学杂志, 2009,28(12):2410-2416.
	CHEN Y, SHI Y, HE X Y. Emission characteristics of biogenic volatile organic compounds from four tree species in Shenyang, China[J]. Chinese Journal of Ecology, 2009,28(12):2410-2416. DOI: 10.13292/j.1000-4890.2009.0420.
[20]	张莉, 王效科, 欧阳志云, 等. 中国森林生态系统的异戊二烯排放研究[J]. 环境科学, 2003,24(1):8-15.
	ZHANG L, WANG X K, OUYANG Z Y, et al. Estimation of isoprene emission from forest ecosystems in China[J]. Chinese Journal of Enviromental Science, 2003,24(1):8-15. DOI: 10.13227/j.hjkx.2003.01.002.
[21]	蒋跃林, 张庆国, 杨书运, 等. 28种园林植物对大气CO₂浓度增加的生理生态反应[J]. 植物资源与环境学报, 2006,15(2):1-6.
	JIANG Y L, ZHANG Q G, YANG S Y, et al. Ecophysiological responses of 28 species of garden plants to atmospheric CO₂ enrichment[J]. Journal of Plant Resources and Environment, 2006,15(2):1-6. DOI: 10.3969/j.issn.1674-7895.2006.02.001.
[22]	LONG S P, AINSWORTH E A, ROGERS A, et al. Rising atmospheric carbon dioxide: plants FACE the future[J]. Annual Review of Plant Biology, 2004,55(1):591-628. DOI: 10.1146/annurev.arplant.55.031903.141610.
[23]	CHEN G Y, YONG Z H, LIAO Y, et al. Photosynthetic acclimation in rice leaves to free-air CO₂ enrichment related to both ribulose-1,5-bisphosphate carboxylation limitation and ribulose-1,5-bisphosphate regeneration limitation[J]. Plant and Cell Physiology, 2005,46(7):1036-1045. DOI: 10.1093/pcp/pci113. doi: 10.1093/pcp/pci113 pmid: 15840641
[24]	陈根云, 廖轶, 蔡时青, 等. 水稻田稗草叶片光合作用对开放式空气CO₂浓度增高(FACE)的适应[J]. 应用生态学报, 2002,13(10):1201-1204.
	CHEN G Y, LIAO Y, CAI S Q, et al. Leaf photosynthetic acclimation of Echinochloa crusgalli grown in rice field to free air CO₂ enrichment (FACE)[J]. Chinese Journal of Applied Ecology, 2002,13(10):1201-1204. DOI: 10.13287/j.1001-9332.2002.0279
[25]	AINSWORTH E A, DAVEY P A, HYMUS G J, et al. Is stimulation of leaf photosynjournal by elevated carbon dioxide concentration maintained in the long term? A test with Lolium perenne grown for 10 years at two nitrogen fertilization levels under Free Air CO₂ Enrichment (FACE)[J]. Plant, Cell and Environment, 2003,26(5):705-714. DOI: 10.1046/j.1365-3040.2003.01007.x.
[26]	AINSWORTH E A, ROGERS A, NELSON R, et al. Testing the “source-sink” hypojournal of down-regulation of photosynjournal in elevated [CO₂] in the field with single gene substitutions in Glycine max[J]. Agricultural and Forest Meteorology, 2004,122(1-2):85-94. DOI: 10.1016/j.agrformet.2003.09.002.
[27]	韩文军, 廖飞勇, 何平. 大气二氧化碳浓度倍增对闽楠光合性状的影响[J]. 中南林学院学报, 2003,23(2):62-65.
	HAN W J, LIAO F Y, HE P. The photosynthetic response of Phoebe bournei to doubled CO₂ concentration in air[J]. Journal of Central South Forestry University, 2003,23(2):62-65. DOI: 10.3969/j.issn.1673-923X.2003.02.009.
[28]	TEGELBERG R, JULKUNEN-TIITTO R, VARTIAINEN M, et al. Exposures to elevated CO₂, elevated temperature and enhanced UV-B radiation modify activities of polyphenol oxidase and guaiacol peroxidase and concentrations of chlorophylls, polyamines and soluble proteins in the leaves of Betula pendula seedlings[J]. Environmental and Experimental Botany, 2008,62(3):308-315. DOI: 10.1016/j.envexpbot.2007.10.003.
[29]	李萍, 郝兴宇, 杨宏斌, 等. 大气CO₂浓度升高对绿豆生长发育与产量的影响[J]. 核农学报, 2011,25(2):358-362, 396.
	LI P, HAO X Y, YANG H B, et al. Effects of air CO₂ enrichment on growth and yield of mung bean[J]. Acta Agriculturae Nucleatae Sinica, 2011,25(2):358-362, 396.
[30]	翟晓朦, 王铁梅, 关潇, 等. 3种秋眠类型苜蓿对不同CO₂浓度的生理响应[J]. 草业科学, 2016,33(8):1550-1559.
	ZHAI X M, WANG T M, GUAN X, et al. Effect of different CO₂ concentrations on physiological responses of fall-dormant alfalfa[J]. Pratacultural Science, 2016,33(8):1550-1559. DOI: 10.11829/j.issn.1001-0629.2016-0128.
[31]	BLOOM A J, BURGER M, RUBIO-ASENSIO J S R,, et al. Carbon dioxide enrichment inhibits nitrate assimilation in wheat and Arabidopsis[J]. Science, 2010,328(5980):899-903. DOI: 10.1126/science.1186440.
[32]	张振, 金国庆, 丰忠平, 等. 马尾松年轮稳定碳同位素比率(δ~(13)C)变化特征及影响因子分析[J]. 植物资源与环境学报, 2019,28(4):24-31.
	ZHANG Z, JIN G Q, FENG Z P, et al. Analyses on variation characteristics and influence factors of ring stable carbon isotope ratio (δ13) of Pinus massoniana[J]. Journal of Plant Resources and Environment, 2019,28(4):24-31.DOI: 10.3969/j.issn.1674-7895.2019.04.03.
[33]	郭世伟, 冉炜, 周毅, 等. 试论大气CO₂浓度升高条件下水稻碳氮代谢变化及其调控途径[J]. 中国水稻科学, 2006,20(5):560-566.
	GUO S W, RAN W, ZHOU Y, et al. On carbon and nitrogen metabolism of rice plants under elevated CO₂ conditions[J]. Chinese Journal of Rice Science, 2006,20(5):560-566. DOI: 10.16819/j.1001-7216.2006.05.019.
[34]	AINSWORTH E A, ROGERS A. The response of photosynjournal and stomatal conductance to rising [CO₂]: mechanisms and environmental interactions[J]. Plant, Cell and Environment, 2007,30(3):258-270. DOI: 10.1111/j.1365-3040.2007.01641.x.
[35]	ENGINEER C B, HASHIMOTO-SUGIMOTO M, NEGI J, et al. CO₂ sensing and CO₂ regulation of stomatal conductance: advances and open questions[J]. Trends in Plant Science, 2016,21(1):16-30. DOI: 10.1016/j.tplants.2015.08.014.
[36]	YUAN J S, HIMANEN S J, HOLOPAINEN J K, et al. Smelling global climate change: mitigation of function for plant volatile organic compounds[J]. Trends in Ecology & Evolution, 2009,24(6):323-331. DOI: 10.1016/j.tree.2009.01.012. pmid: 19324451
[37]	MOCHIZUKI T, WATANABE M, KOIKE T, et al. Monoterpene emissions from needles of hybrid larch F1 (Larix gmelinii var. japonica × Larix kaempferi) grown under elevated carbon dioxide and ozone[J]. Atmospheric Environment, 2017,148:197-202. DOI: 10.1016/j.atmosenv.2016.10.041.
[38]	TIIVA P, JING T, MICHELSEN A, et al. Monoterpene emissions in response to long-term night-time warming, elevated CO₂ and extended summer drought in a temperate heath ecosystem[J]. Science of the Total Environment, 2017,580:1056-1067. DOI: 10.1016/j.scitotenv.2016.12.060.
[39]	KLAIBER J, NAJAR-RODRIGUEZ A J, PISKORSKI R, et al. Plant acclimation to elevated CO₂ affects important plant functional traits, and concomitantly reduces plant colonization rates by an herbivorous insect[J]. Planta, 2013,237(1):29-42. DOI: 10.1007/s00425-012-1750-7.
[40]	NIINEMETS Ü. Stomatal constraints May affect emission of oxygenated monoterpenoids from the foliage of Pinus pinea[J]. Plant Physiology, 2002,130(3):1371-1385. DOI: 10.1104/pp.009670. doi: 10.1104/pp.009670 pmid: 12428002
[41]	NIINEMETS Ü, LORETO F, REICHSTEIN M. Physiological and physicochemical controls on foliar volatile organic compound emissions[J]. Trends in Plant Science, 2004,9(4):180-186. DOI: 10.1016/j.tplants.2004.02.006.
[42]	LORETO F, CICCIOLI P, CECINATO A, et al. Evidence of the photosynthetic origin of monoterpenes emitted by Quercus ilex L. leaves by ¹³C labeling [J]. Plant Physiology, 1996,110(4):1317-1322. DOI: 10.1104/pp.110.4.1317.
[43]	JARDINE A B, JARDINE K J, FUENTES J D, et al. Highly reactive light-dependent monoterpenes in the Amazon[J]. Geophysical Research Letters, 2015,42(5):1576-1583. DOI: 10.1002/2014gl062573.
[44]	PE Ñ UELAS J, LLUSIA J. Seasonal emission of monoterpenes by the Mediterranean tree Quercus ilex in field conditions: Relations with photosynthetic rates, temperature and volatility[J]. Physiologia Plantarum, 1999,105(4):641-647. DOI: 10.1034/j.1399-3054.1999.105407.x.

Metrics

Comments

www.nldxb.njfu.edu.cn

Edited ＆ Published by Editorial Department of Nanjing Forestry University(Natural Sciences Edition)
Address： No.159 Longpan Road,Nanjing 210037 Jiangsu,P.R.China
E-mail ：xuebao@njfu.edu.cn , xuebao@njfu.com.cn
Telephone +86-25-85428247,85427076
Distributed by China International Book Trading Corporation P.O. Box 399, Beijing ,P.R. China

CO₂浓度/ (μmol·mol^-1) CO₂ concentration	10月叶绿素含量/(mg·g^-1) Chl contents during Oct.			11月叶绿素含量/(mg·g^-1) Chl contents during Nov.
CO₂浓度/ (μmol·mol^-1) CO₂ concentration	Chl a	Chl b	Chl	Chl a	Chl b	Chl
437(CK)	1.19±0.22 a	0.56±0.12 a	1.76±0.31 a	1.28±0.14 a	0.43±0.05 a	1.71±0.19 a
550	1.23±0.23 a	0.53±0.21 a	1.76±0.29 a	1.16±0.16 ab	0.37±0.05 ab	1.52±0.21 ab
750	1.20±0.27 a	0.46±0.10 a	1.66±0.37 a	1.06±0.16 bc	0.35±0.05 bc	1.41±0.21 bc
1 000	1.10±0.19 a	0.41±0.07 a	1.51±0.26 a	0.90±0.11 c	0.30±0.04 c	1.20±0.14 c

Effects of elevated CO₂ on photosynthetic characteristics and monoterpene emissions in Pinus massoniana seedlings

RichHTML

PDF

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 4

References 44

Related Articles 15

Recommended Articles

Metrics

Comments

Author

Reader

Reviewer

[1]	WU Yan, HUANG Qing, LIU Xun, ZHENG Rui, CEN Jiabao, DING Bo, ZHANG Yunlin, FU Yuhong. Effects of Pinus massoniana plantation age on soil physical and chemical properties in Karst areas in southwest China [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2024, 48(3): 99-107.
[2]	FAN Mingyang, HU Meng, YNAG Yuan, FANG Yanming. Community classification, structures and species diversity characteristics of Pinus massoniana and P. hwangshanensis in the eastern China [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2024, 48(1): 47-58.
[3]	WEI Yi, WEI Xiaoli, WANG Mingbin, WANG Man, YU Dalong. Effects of elevated atmospheric CO₂ concentration on the photosynthetic physiology and morphology of Ormosia hosiei seedlings [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2023, 47(6): 124-132.
[4]	WANG Zhangrong, JI Kongshu, XU Li’an, ZOU Bingzhang, LIN Nengqing, LIN Jingquan. New management model of construction techniques, realistic genetic gain and low cost multi-generation improvement in seedling seed orchard of Pinus massoniana [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2023, 47(6): 9-16.
[5]	WANG Yu, YI Yanling, LIU Hai, WEN Xiaochen, LI Tianyi, YIN Haifeng, LI Xianwei, FAN Chuan. Initial impacts of two thinning methods on the spatial structure of Pinus massoniana plantations [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2023, 47(5): 138-146.
[6]	ZHU Lei, XU Junliang, ZHANG Yiping, LUO Pengfei, SHI Zhiqiang, HOU Jiayu, ZHAI Lexin. Analysis on diurnal variation of sap flow in Pinus massoniana and its influencing factors in Luoyang, Henan Province, China [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2023, 47(1): 92-100.
[7]	SUN Wei, WANG Bin, CHU Xiuli, WANG Xiuhua, ZHANG Dongbei, WU Xiaolin, ZHOU Zhichun. Response and association of the growth and nutrient traits of Pinus massoniana container seedlings to phosphorus addition and inoculation of mycorrhizal fungi [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2023, 47(1): 226-233.
[8]	JI Kongshu, XU Li’an, WANG Dengbao, NI Zhouxian, WANG Zhangrong. Progresses and achievements of genetic improvement on Masson pine (Pinus massoniana) in China [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2022, 46(6): 10-22.
[9]	HU Xingfeng, WU Fan, SUN Xiaobo, CHEN Houping, YIN Anzheng, JI Kongshu. Joint analysis of growth and wood property of 38-year-old Pinus massoniana from 55 provenances [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2022, 46(3): 203-212.
[10]	WU Fan, ZHU Peihuang, JI Kongshu. Responses of masson pine(Pinus massoniana) distribution patterns to future climate change [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2022, 46(2): 196-204.
[11]	GAO Jingbin, XU Liuyi, YE Jianren. Growth and genetic diversity analysis of clones screened by phenotypical resistant to pine wilt disease in Pinus massoniana [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2021, 45(5): 109-118.
[12]	YIN Yannan, TAN Jiajin, LI Mengwei, XU Jialin, HAO Dejun. A study on the biocontrol of pine wilt disease by Bacillus cereus NJSZ-13 [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2021, 45(3): 152-158.
[13]	LUO Bizhen, HU Haiqing, LUO Sisheng, WEI Shujing, WU Zepeng, LIU Fei. Effects of forest fire disturbance on soil organic carbon density and labile organic carbon of Pinus massoniana forests in Guangdong Province, China [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2020, 44(5): 132-140.
[14]	SUN Xiaobo, CHEN Peizhen, WU Xiaogang, WU Fan, JI Kongshu. The cloning and expression analysis of PmAOX gene from Pinus massoniana under different stress [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2020, 44(4): 70-78.
[15]	ZHOU Sijie, WANG Ping, ZHANG Min, CHEN Shuzhan, XU Wen, ZHU Liting, HE Xiaoqin, GONG Shurui. Effects of atmospheric acid deposition on root physiological characteristics of Pinus massoniana seedlings [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2020, 44(4): 111-118.