Laser microdissection provides a rapid isolating and purification modern method for biological tissues and cellular preparations. It directly cuts objects from heterogeneous tissues under the microscope. This review concerned an up-to-date advance in Laser microdissection techniques which are applied on to the plant cell and molecular biology researches in regard with the sample preparation, gene expression analysis, DNA and protein studies etc. Meanwhile, possible directions for future development of laser microdissection in plant research were put forward.