Differential proteomic analysis on dormant and dormancy releasing seeds of Cercis chinensis

doi:10.12302/j.issn.1000-2006.202304028

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2025, Vol. 49 ›› Issue (3): 137-143.doi: 10.12302/j.issn.1000-2006.202304028

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Differential proteomic analysis on dormant and dormancy releasing seeds of Cercis chinensis

SUN Yonglian¹(), GAO Yunpeng¹, HOU Jing¹, WANG Wenwu², WU Xuelian², LI Shuxian^*()

1. State Key Laboratory of Tree Genetics and Breeding, Co-Innovation Center for Sustainable Forestry in Southern China, Key Laboratory of Tree Genetics and Biotechnology of Educational Department of China, Key Laboratory of Tree Genetics and Silvicultural Sciences of Jiangsu Province, Nanjing Forestry University, Nanjing 210037, China
2. Xiangyang Forestry Science and Technology Extension Station of Hubei Province, Xiangyang 441022, China

Received:2023-04-23 Accepted:2023-07-04 Online:2025-05-30 Published:2025-05-27
Contact: LI Shuxian E-mail:syl@njfu.com.cn;shuxianli@njfu.com.cn

Abstract

Abstract:

【Objective】This study investigated proteom-level changes between dormant and broken dormancy seeds of Cercis chinensis to get a better understanding of the intrinsic mechanisms underlying seed dormancy and germination.【Method】The mature seeds were soaked in hot water until they reached a constant weight, and then subjected to stratification treatment mixed with moisture sand in a 4 ℃ environment until dormancy was released and germination occurred. Label-free quantitative proteomics and liquid chromatography-tandem mass spectrometry (LC-MS/MS) were used to perform proteome quantitative and qualitative analysis on dormant and dormancy-broken seeds after 45 days of cold stratification were performed based on, respectively. Bioinformatics analysis was also conducted to explore the expression and function mechanism of differentially expressed proteins (DEPs) between dormant and dormancy-broken seeds, and to discuss the proteins and its mechanisms related to the dormancy release.【Result】A total of 3 928 proteins were identified in this study, with 3 122 proteins were detected in dormant samples and 3 758 proteins were detected in dormancy-broken samples. After screening the proteins based on the standard of fold changes greater than 2.0 and P<0.05, we filtered 1 031 DEPs. Among these, 779 proteins were up-regulated and 252 proteins were down-regulated. The DEPs were annotated using GO annotation into three categories and 49 subcategories of biological processes, cellular components and molecular functions. The annotated DEPs were closely related to metabolic processes, enzyme catalytic activity, synthesis of cellular components, and stress response. In addition, the KEGG metabolic pathway annotation results showed that 1 012 DEPs were annotated, involving 264 pathways. The DEGs were mainly associated with carbon metabolism, polysaccharide decomposition, and protein processing. The significant enrichment results revealed seven pathways, mainly involving the hormone synthesis, secondary metabolite synthesis, and lipid metabolism. The highly abundant DEPs that may be linked to dormancy breaking are proteins related to β-glucosidase activity, ubiquinone, terpene quinone organisms related proteins, and glyoxylic acid cycle-related proteins. 【Conclusion】This study demonstrates that the dormancy release of C. chinensis seeds is a complex biological process that involves cell morphological changes, enzyme catalysis, polysaccharide decomposition and hormone signal transduction. The release of dormancy is regulated by the interaction of multiple metabolic pathways. Further research is needed to study the molecular mechanism of dormancy release using molecular biological methods such as transcriptomics and metabonomics.

Key words: Cercis chinensis, seed dormancy, proteome, metabolic pathway

CLC Number:

S722.1

SUN Yonglian, GAO Yunpeng, HOU Jing, WANG Wenwu, WU Xuelian, LI Shuxian. Differential proteomic analysis on dormant and dormancy releasing seeds of Cercis chinensis[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2025, 49(3): 137-143.

Figures/Tables 6

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Table 1

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通路编号 pathway ID	KEGG通路 KEGG pathway	DEPs 表达数 number of DEPs	P
ko00140	类固醇激素生物合成 steroid hormone biosynthesis	3	0.014 929 584
ko00966	硫代葡萄糖苷生物合成 glucosinolate biosynthesis	3	0.014 929 584
ko00750	维生素B6代谢 vitamin B6 metabolism	4	0.035 630 834
ko00592	D-亚麻酶代谢 alpha-Linolenic acid metabolism	10	0.036 770 191
ko05205	癌症中的蛋白聚糖 proteoglycans in cancer	5	0.046 007 184
ko05202	癌症中的转录调控失调 transcriptional misregulation in cancers	5	0.046 007 184
ko04934	库欣综合征 Cushing’s syndrome	3	0.048 705 937

Differential proteomic analysis on dormant and dormancy releasing seeds of Cercis chinensis

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