Abstract: 【Objective】Determine the suitable formula and understand the mechanism of rooting Altingia gracilipes cuttings to improve cutting survival rate and establish a cutting propagation system.【Method】In this study, a total of 13 treatment groups using four different concentrations(150,300,500 and 800 mg/L)of IAA, NAA or GGR and one water-treated control(CK)were used to treat 3-year-old Altingia gracilipes softwood seedlings. The rooting process, rooting(rooting rate, average root number, average root length)and the dynamic changes in nutrient content(soluble protein, soluble starch, soluble sugar)and oxidase activity(peroxidase(POD),polyphenol oxidase(PPO),indoleacetic acid oxidase(IAAO))were observed during the rooting process of Altingia gracilipes cuttings.【Result】①Plant growth regulators had a significant influence on rooting time and rooting of Altingia gracilipes cuttings. ②Rooting of cuttings in the treatment with 300 mg/L NAA for 2 h were the earliest to exhibit the rooting process, 12 d ahead of CK, and the rooting rate(78%),average root number(16.8),and average root length(4.27 cm),were, 3.4, 6.2 and 4.6 times that of CK. ③Dynamic changes in nutritive material content and oxidase activity in the process of Altingia gracilipes rooting from the adventitious root induction period to the early adventitious root formation,than the adventitious root formation stage, indicated that the peak or valley value of each indicator appeared at the same time as the beginning time of the early adventitious root or adventitious root formation stage, which reflected the synergy between the rooting process and physiological metabolism. ④The 300 mg/L NAA treatment significantly improved oxidase activities, the accumulation of soluble proteins.【Conclusion】Plant growth regulators sped up the process of synthesis and metabolism of nutrients and rooting, and improved the ability to regulate endogenous oxidase.