Study on the physiological and biochemical changes of Phoebe sheareri seed during its dormancy breaking

doi:10.12302/j.issn.1000-2006.202110038

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2023, Vol. 47 ›› Issue (2): 9-17.doi: 10.12302/j.issn.1000-2006.202110038

Special Issue: “攥紧中国种子”视域下的中国林草种业研究专题Ⅰ

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Study on the physiological and biochemical changes of Phoebe sheareri seed during its dormancy breaking

LIU Miao¹(), GAO Handong¹^,^*(), GAO Yan², XUE Xiaoming³

1. College of Forestry, Nanjing Forestry University, Southern Tree Seed Inspection Center, National Forestry and Grassland Administration, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing 210037, China
2. College of Science, Nanjing Forestry University, Nanjing 210037, China
3. Nanjing Forest Police College, College of Criminal Science and Technology, Key Laboratory of Wildlife Evidence Technology of National Forestry and Grassland Administration, Nanjing 210023, China

Received:2021-10-18 Revised:2022-05-23 Online:2023-03-30 Published:2023-03-28

Abstract

Abstract:

【Objective】 In this study, experiments were carried out to explore the dynamic changes of nutrient contents, enzyme activities and endogenous hormones during the seed stratification of Phoebe sheareri. Additionally, this study was conducted to reveal the internal physiological mechanisms involved in seed dormancy and provide a theoretical basis for its dormancy release and germination. 【Method】 Fresh and full seeds of P. sheareri were treated with gibberellin and stratification was conducted; the physiological indicators during stratification were also determined. 【Result】 The seed dormancy of P. sheareri was relieved by stratification for 63 days. The germination rate of low temperature and variable temperature stratification was 85.83% and 72.75%, respectively. The initial starch content was 467.88 mg/g and decreased gradually during stratification. Soluble sugar, soluble protein and crude fat were decomposed to varying degrees. Under the two kinds of stratification treatments, the soluble sugar content in the seeds of P. sheareri changed greatly, from decreasing to increasing, and then decreasing again. The soluble protein content in P. sheareri seeds increased initially and then decreased under two stratification treatments. On the whole, the crude fat content in the seeds of P. sheareri showed a gradually decreasing trend under each stratification treatment. The activities of amylase, APA and POD were increased. This indicated that the two stratification treatments could promote the continuous activation of APA and POD activities. What’s more, endogenous hormones such as IAA, IPA, GA₃ and ABA played an important role in the regulation of seed dormancy and germination. During the whole stratification process, the contents of IAA, IPA and GA₃ increased, while the contents of ABA decreased, and the mass ratios of IAA/ABA, IPA/ABA and GA₃/ABA increased. 【Conclusion】 There was medium dormancy in seeds of P. sheareri. Gibberellin and stratification could release dormancy, and cold stratification was better to accelerate seed germination. Starch was the main storage material in the seeds of P. sheareri. The transformation of nutrients and the coordination of endogenous hormones played an important role in the seeds dormancy release.

Key words: Phoebe sheareris, seeds, germination, dormancy, physiological and biochemical indicators

CLC Number:

S722

LIU Miao, GAO Handong, GAO Yan, XUE Xiaoming. Study on the physiological and biochemical changes of Phoebe sheareri seed during its dormancy breaking[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2023, 47(2): 9-17.

Figures/Tables 5

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Study on the physiological and biochemical changes of Phoebe sheareri seed during its dormancy breaking

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