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    Spatial-temporal change of leaf area index(LAI)of vegetations in Hebei Province from 2002 to 2011
    ZHANG Yujia, YUAN Jinguo, ZHANG Sha
    JOURNAL OF NANJING FORESTRY UNIVERSITY    2015, 39 (01): 86-92.   DOI: 10.3969/j.issn.1000-2006.2015.01.016
    Abstract6456)      PDF(pc) (2410295KB)(215725)       Save
    The vegetation index product MOD15A2 and vegetation type product MCD12Q1 of EOS/MODIS data and meteorological data were used in this study. LAI of seven typical vegetation types were compared,and spatial temporal change of LAI in growing seasons in Hebei province from 2002 to 2011 was explored by using linear regression analysis and correlation analysis methods. The relationship between LAI, air temperature and precipitation were also analyzed. The results showed that the annual average LAI values in growing seasons(from May to October)were 1.0-1.3, the highest was 1.286 in 2008, and the lowest was 1.066 in 2002. As for spatial distribution, LAI in Chengde region in the northeast was the highest, which reached more than 5.0, and LAI in Zhangjiakou region in the northwest was the lowest, which reached less than 0.5. The annual average LAI of seven typical vegetation types in growing seasons were very different, and LAI from high to low could be broadleaf forest(2.765), mixed forest(2.535), shrub(1.867), savanna(1.476), coniferous forest(1.331), farmland(1.116)and grassland(0.967). The significant factors influencing LAI were precipitation, air temperature, topography, vegetation types and phenology, and the impact of each factor on vegetation LAI was different.
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    Rapid detection method for land use change based on remote sensing images segmentation units
    WANG Zhijie
    JOURNAL OF NANJING FORESTRY UNIVERSITY    2015, 39 (03): 1-5.   DOI: 10.3969/j.issn.1000-2006.2015.03.001
    Abstract6901)      PDF(pc) (3006367KB)(130461)       Save
    Because of actual demand of land use change supervision, a method for quick detection of land use change is presented. This is the analysis of the remotely sensed imagery that is overlapped with corresponding land-use map. The image is segmented by pattern spots in the presented land-use map, and the gray level of pixel of every segment unit is obtained by statistics and analysis. Then, the land use of pattern spot is judged whether it has changed. Finally, the changed pattern spots in remotely sensed imagery are extracted. The high-resolution remotely sensed imagery of 2013 and present land-use map of 2012 from a certain county are selected as an example to extract the section. Results showed that among 938 experiment pattern spots, 12 pattern spots are judged to be changed, among 12 pattern spots, eight of them are changed actually, four of them are not changed actually, and no actually changed pattern spots are ignored. The experiment showed that the method could quickly and effectively detect and extract the information of land use change, especially was suit for the situation of farmland which was changed to be construction land.
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    A review on the management models and comprehensive evaluation methods for agroforestry in China
    ZHU Ling, ZHOU Yuxin, TANG Luozhong, TONG Tingting
    JOURNAL OF NANJING FORESTRY UNIVERSITY    2015, 39 (04): 149-156.   DOI: 10.3969/j.issn.1000-2006.2015.04.026
    Abstract6234)      PDF(pc) (1556818KB)(116461)       Save
    Agroforestry is regarded as an efficient, intensive and ecological land management pattern in rural area. Because of the long history in agriculture and the multiple conditions of nature, diversified agroforestry models were developed in China. Agroforestry could expand the ways of agriculture management, optimize the structures of rural economy, and improve land productivity and sustainability. However, inappropriate agroforestry management models could also intensify the pressure to land managers and environment. Therefore, it is necessary to evaluate existing models of agroforestry management and choose appropriate ones by using scientific methods. In China, agroforestry mainly includes four models such as forestry-agriculture based, forestry-livestock based, forestry-fisheries based and integrated type. Comprehensive evaluation methods on the agroforestry mainly include analytic hierarchy process(AHP), grey correlation analysis, principal component analysis, AHP—fuzzy evaluation method. In future, it is necessary to strengthen the research on the agroforestry pattern classification, comprehensive evaluation and management techniques.
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    Landscape ecological quality change of Anhui Province in 2000-2010
    WU Jian, ZHANG Yan, HOU Langong
    JOURNAL OF NANJING FORESTRY UNIVERSITY    2015, 39 (02): 84-90.   DOI: 10.3969/j.issn.1000-2006.2015.02.015
    Abstract6717)      PDF(pc) (2226228KB)(110576)       Save
    Landscape ecological quality is an important index to measure the ecological environment. There is greatly significance to study the landscape ecological quality from the angle of landscape ecology. Landscape ecological quality depends on the landscape ecosystem, and landscape ecosystem is influenced by its stability and external interference degree mainly. The evaluation index system was established from the two aspects which are stability and interference degree. Then a comprehensive evaluation model was built to evaluate the landscape ecological quality and its changes in Anhui Province in recent ten years. The results showed that the area of landscape ecological quality as the first class only decreased by 0.84%, and the area of landscape ecological quality as the third class only increased by 0.54% in 2000-2005. But in 2005-2010, the area of landscape ecological quality as the first class decreased by 2.46%, and the area of landscape ecological quality as the third class increased by 4.33%. Which showed that landscape ecological quality of Anhui Province decreased from 2000 to 2005, but the overall situation was still in a stable state. While the landscape ecological quality was in a declining trend from 2005 to 2010.
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    Research on the features of DNA methylation in embryo ripening period ofFraxinus mandshurica Rupr. based on the method of MSAP
    CONG Jianmin,CHEN Fengqing,SHEN Hailong2,4*,LI Yuhua3,4, ZHANG Peng2,4,YANG Ling2,4
    JOURNAL OF NANJING FORESTRY UNIVERSITY    2015, 39 (03): 39-44.   DOI: 10.3969/j.issn.1000-2006.2015.03.008
    Abstract6168)      PDF(pc) (1485460KB)(109208)       Save
    To research epigenetic regulation of genome of Fraxinus mandshurica seed development ripening period, Fraxinus mandshurica cotyledons from early July to mid-September 8 stages were selected as test material. Through MSAP(methylation-sensitive amplified polymorphism)method, we detected the change of DNA ethylation in each period. The result indicated that no significant difference among different development stages was detected(in total 933-939 clearly identified strips was detected in each stage). However, the cytosine methylation patterns and variation pattern of CCGG sites showed that, in early July, CNG, CG/CNG loci demethylation sites were higher than other stage, which indicated that large scale of DNA demethylation occurred following seed development of Fraxinus mandshurica. This result could serve as the regulation patterns and as an important basis for providing theoretical basis for seed development.
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    Soil quality comprehensive assessment of different plant communities in Shanghai green belt
    ZHANG Kaixuan, SHANG Kankan, DA Liangjun
    JOURNAL OF NANJING FORESTRY UNIVERSITY    2015, 39 (03): 71-77.   DOI: 10.3969/j.issn.1000-2006.2015.03.014
    Abstract5691)      PDF(pc) (1474960KB)(100330)       Save
    To explore the soil improvement service of plant communities, and provide a scientific basis for urban forest planning and management, soil samples were collected from seven plant communities in Shanghai green belt with bare lands as the control. Eight soil physical and chemical indicators were measured in different soil depth, including soil bulk density, capillary porosity, total porosity, organic matter, total N, total P, NH+4-N and NO-3-N. Principal component analysis was used to assess the comprehensive soil quality based on those indicators. Results showed that soil improvements were mainly on the surface of soil(0-20 cm), with different soil improvement effects of each plant communities. Deciduous broad leaved forest with Platanus×hispanica as dominate species and deciduous coniferous forest with Taxodium distichum var. imbricarium as dominate species had the greatest improvement effect, deciduous broad-leaved forest with Koelreuteria bipinnata var. integrifoliola as dominate species had the least improvement effect, and the other communities were in the middle level. Lower-intense-management was helpful to litter layer accumulating, and facilitate the recruitment of understory seedlings and herbs, which could contribute to the overall improvement of soil quality.
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    Variation in bark quality among eleven populations of Pteroceltis tatarinowii Maxim. and their relationship with environmental factors
    LIU Huijun, ZHANG Li, WANG Fang, JIANG Xiao, ZHANG Xiaoping
    JOURNAL OF NANJING FORESTRY UNIVERSITY    2015, 39 (03): 78-84.   DOI: 10.3969/j.issn.1000-2006.2015.03.015
    Abstract5753)      PDF(pc) (1519456KB)(99677)       Save
    In the paper, the relationship between the bark qualities of wingceltis(Pteroceltis tatarinowii)and ecological environment factors in the collecting locations was determined by redundancy analysis(RDA). The detected bark quality indexes contained cellulose content, lignin content, pentosan content and fiber morphology,while ecological environment factors contained soil pH, organic matter, mineral elements and climatic conditions. Wingceltis in the study was collected from six provinces and one city. The following results were obtained: wingceltis adapted to the environment which was more soil organic matter, higher calcium and lower phosphorus. The bark cellulose content, lignin content, fiber length and width in different populations had significant difference, in addition, the cellulose contents and fiber length could be remarkably influenced by climatic and soil factors. Concretely, the bark quality from the populations in Guizhou Anlong, Yunnan Luoping and Anhui Jingxian was the best owing to higher cellulose content, longer fiber length and lower lignin content. Besides, the cellulose content was mainly affected by frost free period, the latitude, the annual average temperature, soil organic matter and available P; while the main influence factor of the fiber length was soil available K. We could reach our conclusion that as Xuan paper materials, the bark quality from the populations in low latitude area was better than that in high latitude area universally.
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    BASIC PRINCIPLES AND METHODS TO DETERMINE WOOD DENSITY UNDER X-RAY
    Wang Wanhua
    JOURNAL OF NANJING FORESTRY UNIVERSITY    1984, 8 (02): 114-119.   DOI: 10.3969/j.jssn.1000-2006.1984.02.016
    Abstract5268)      PDF(pc) (1504574KB)(91917)       Save
    This paper introduces the basic principles and methods of X-ray densitometry used at the French National Forestry Research Centre, in which the author had a two-week stay and conducted some experiments under Professor Polge’s guidance.Testing results with Chinese-fir wood (from China) as samples showed that the average annual density changed slightly as the radial diameters of the tree increased and the average annual density differed only by 16.59 percent, while the maximum ring width was 2-18 times larger than the minimum ring width. Thus the author drew a conclusion that the Chinese-fir plants which grow faster would have evener wood quality and an intensive silvicultural system should be adopted to obtain more yeariy increments of the trees.
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    Induction and proliferation of embryogenic callus in nematode-resistant Pinus densiflora
    WU Jing, ZHU Lihua,XU Jianxiu, WU Xiaoqin, YE Jianren
    JOURNAL OF NANJING FORESTRY UNIVERSITY    2015, 39 (01): 17-21.   DOI: 10.3969/j.issn.1000-2006.2015.01.004
    Abstract4927)      PDF(pc) (1848996KB)(86255)       Save
    Embryogenic callus was induced from immature embryos of nematode-resistant Pinus densiflora. Factors affecting callus initiation were investigated. The results showed that successful initiation of embryogenic callus depended heavily on embryo developmental stage, the combination of auxin and cytokinins, and carbon source. The initiation of embryogenic callus worked best when immature mega gametophytes developed at stage 2-4 were used as starting material. The optimal combination of hormone was 2 mg /L 2,4-D and 1 mg /L 6-BA with the induction rate of 5%. Maltose was better than sucrose and glucose. Further proliferation of embryogenic callus was achieved by transfering explants to a medium with reduced levels of plant growth regulators.
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    A quantitative analysis of the heritage resources of Nanjing Ming Dynasty City Wall corridor based on AHP
    WANG Yanyan,WANG Hao
    JOURNAL OF NANJING FORESTRY UNIVERSITY    2015, 39 (04): 95-100.   DOI: 10.3969/j.issn.1000-2006.2015.04.016
    Abstract4729)      PDF(pc) (2323257KB)(65072)       Save
    Based on the survey of the heritage resources along the Nanjing Ming Dynasty City Wall, the paper registered and evaluated the corridor heritage resources. AHP and Delphi were applied to establish the three-level index evaluation system of the heritage resources, including their values, their possibility of integration with the city functions and their influential power. The weight of each index factor was determined and the comprehensive scores were also sequenced. Through qualitative analysis, the heritage resources to be registered were determined and the distribution of the registered heritage resources, the protection level and the present condition were analyzed. The author thinks that the distribution of the heritage resources is closely related to the historic development and the geographical environment of Nanjing City. The current protection status is also greatly related to level of the heritage resources and their integration with the city functions.
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    Analysis of errors introduced by DEM in geoid refinement
    WANG Zengli, LIU Xuejun
    JOURNAL OF NANJING FORESTRY UNIVERSITY    2014, 38 (01): 145-150.   DOI: 10.3969/j.issn.1000-2006.2014.01.026
    Abstract4904)      PDF(pc) (4449516KB)(29625)       Save
    There’re several factors affecting the accuracy of terrain correction to obtain geoid with centimeter-level: accuracy of DEM,resolution and the integral radius. Derive the linear relationship between DEM and terrain correction by computing the spherical harmonic expansions. The result showed that the accuracy of DEM should be better than 81m. After the relationship between resolution and the degree of spherical harmonic expansions and integral radiusanalyzed, the best suitable factors for geoid with centimeter-level was giant.This article chosed different resolution and different integral radius to compute terrain correction with DEM of Tibet. The test results showed that the resolution of 6″ DEM could meet the needs of geoid with centimeter-level. And it needs 50’ integral radius to meet the corresponding requirements.
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    The fecundity and virulence of offspring crossbred from virulent and avirulent isolates of Bursaphelenchus xylophilus
    ZHU Lihua, SHI Xuewen, HU Yudan, LIANG Wen, YE Jianren
    JOURNAL OF NANJING FORESTRY UNIVERSITY    2015, 39 (01): 1-5.   DOI: 10.3969/j.issn.1000-2006.2015.01.001
    Abstract5349)      PDF(pc) (1453198KB)(27222)       Save
    In order to understand the genetic variation in pine wood nematode, virulent and avirulent isolates of Bursaphelenchus xylophilus were used as parental isolates to establish crossbred lines, and the virulence and reproductive ability of offsprings were compared with parental isolates. The results showed that hybrid rates were different among different parents, even one pair parents for reciprocal cross. A significant difference was observed in reproductive ability among parental isolates and some crossbred lines on Botrytis cinerea and in masson pine seedlings. The proportion of dead masson pine seedlings inoculating with virulent parent and crossbred lines ranged from 90%-100% after 5 weeks, while only 20% of the seedlings inoculating with avirulent parent showed slight wilting symptons 10 weeks later. Statistical analyses revealed that virulence was positively correlated with propagation rate inside the host pine seedlings in some extent, but was weakly correlated with propagation rate on B. cinerea. Cluster analysis showed that the biological parameters varied between the parents and offsprings, with no kinship bias, suggesting the absence of sex-linked inheritance in virulence and rates of propagation.
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    Studies on the relationship between Cerasus serrulata distribution region and the environmental factors
    LI Meng, YI Xiangui, WANG Huachen, SHANG Tao, GU Yu, WANG Xianrong
    JOURNAL OF NANJING FORESTRY UNIVERSITY    2014, 38 (增刊): 74-80.   DOI: 10.3969/j.issn.1000-2006.2014.S1.016
    Abstract4907)      PDF(pc) (1558368KB)(17851)       Save
    Based on the geographical distribution information of Cerasus serrulata, the interaction of vegetations and climates have been studied by 13 widely used indices. The results showed, the distribution region of C. serrulata was temperate East Asian, it was 23-40°N, 102-141°E, and the elevation range was 50-1 800 meters. The climatic indices showed that the tree have a low heat range,and the range of heat distribution in China is much wider than in the Korean Peninsula-Japan distribution. The result of principal component analysis revealed that annual mean temperature,latitude,coldest temperature,average temperature of January and elevation ranges was the most important environment factors to influence the distribution. The suitable heat factors for the growth of C. serrulata was the range of annual mean temperature between 7.91-17.92 ℃, coldest temperature not less than 17.3 ℃, average temperature of January between 6.31 ℃ and 8.48 ℃. Supported by the two dimensional plot of principle components on climatic factors, we divided the distribution region into 7 parts, including the region of Southwest China, Central China, north of South China and south of East China, Northeast China, Taiwan of China, Korean Peninsula, Japan.
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    Nitrogen resorption machanism during leaf senescence in woody plants
    YANG Jiading, LIU Yujie, FENG Jianyuan, ZHANG Yuanlan
    JOURNAL OF NANJING FORESTRY UNIVERSITY    2023, 47 (5): 1-8.   DOI: 10.12302/j.issn.1000-2006.202212004
    Abstract1151)   HTML52)    PDF(pc) (1492KB)(16763)       Save

    The application of nitrogen fertilizer is the main driving force for biomass production in artificial systems of agriculture and forestry. However, problems such as the increased cost and substantial pollution of soil, water and atmosphere are resulted from nitrogen leaching. Improving plant nitrogen use efficiency (NUE) is the crucial way to resolve these problems. NUE is a complex trait associated with an interplay between the genetic background and environmental factors, and is the comprehensive reflection of respective efficiencies with uptake of soil nitrogen by plant roots, assimilation and utilization of absorbed nitrogen inside plants, and resorption of nitrogen from senescent tissues or organs. The physiological steps of nitrogen resorption include a protein degradation in senescing organs,amino acid interconversion, and phloem loading of amino acids for remobilization. Phloem loading is thought to be the rate-limiting step of nitrogen resorption from senescing leaves. Nitrogen resorption is always accompanied with either natural senescence of the whole plant or certain tissues/organs determined by a developmental age or precocious senescence induced by environmental stresses. Nitrogen resorption in senescing leaves is an important strategy for perennial woody plants to conserve intrinsic nitrogen and increase stress tolerance through the winter season. The efficiency of nitrogen resorption is not only associated with species, age and functional types (i.e. deciduous or evergreen, broad- or needle-leaved) of woody plants, but also influenced by environmental factors such as geographic habitat, status of soil nitrogen and water supply. Improving efficiency of nitrogen resorption from senescing leaves may alleviate the requirement of trees for nitrogen fertilizer in the next growth season. As studies about mechanisms of leaf senescence and nitrogen resorption in trees are rare at present, characterization of key factors (such as NAC and WRKY family members) regulating leaf senescence, certain transporters responsible for phloem loading of amino acids in senescing leaves and signaling components mediating functions of phytohormones (such as abscisic acid and ethylene) which may promote leaf senescence is desired in woody plants in coming years, thus providing putative technological supports for breeding tree cultivars with the improved NUE through the genetic modification.

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    Recent advances in plant immune system
    CHEN Ying, TAN Biyue, HUANG Minren*
    JOURNAL OF NANJING FORESTRY UNIVERSITY    2012, 36 (01): 129-136.   DOI: 10.3969/j.jssn.1000-2006.2012.01.027
    Abstract4943)      PDF(pc) (1679274KB)(16020)       Save
    Many plantassociated microbes are pathogens that impair plant growth and reproduction. Plants have evolved a series of complicated defense mechanism against pathogens during their long term process of coevolution. Plants innate immune system efficiently detects and wards off potentially dangerous microbes. A first layer of this system is based on sensitive perception of pathogenor microbeassociated molecular patterns (PAMPs) through pattern recognition receptors (PRRs) at the plants cell surface,which would active mitogenactivated protein kinase(MAPK) signaling cascade and burst early responses in plant resistance to pathogen attacks. The first layer recognizes and responds to many classes of microbes, including nonpathogens. With pathogens produce effectors to inhibit PTI, plants can perceive such effectors through additional receptors, typically nucleotide binding leucinerich repeat (NBLRR) proteins, to mount a second layer of defense called effect or triggered immunity (ETI). Here, we highlight recent literature on plants innate immune system, and based on a detailed understanding of plant immune function, we should focus on the combination of the use of PTI and ETI to expand the antimicrobial spectrum of plant effectively and improve plantss diseaseresistance.
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    Response of forest soil greenhouse gases fluxes to forest management and global atmospheric change
    GAO Fei, GAO Lei, CUI Xiaoyang
    JOURNAL OF NANJING FORESTRY UNIVERSITY    2017, 41 (04): 173-180.   DOI: 10.3969/j.issn.1000-2006.201603051
    Abstract3155)      PDF(pc) (1766580KB)(12505)       Save
    Forest soils are important sources and sinks of greenhouse gases(GHG). The objectives of this study were to explore the characteristics of forest soil GHG fluxes under different forest management practices and global atmospheric change, and to provide a reference for the reduction of GHG release and sustainable forest management. Publications were searched by using China Knowledge Resource Integrated Database, Wanfang Database, Science Direct, and Springer Link, using three keywords “forest soil greenhouse gases”, “forest management”, and “atmospheric change”. Papers reporting on forest soil GHGs were analyzed, and the general pattern of forest soil GHG fluxes under different forest management practices and global atospheric change scenarios was analyzed. CO2, CH4 and N2O are three important GHGs, and the coupling between them are complex including synergism, antagonism and random interactions. Forest soil GHG fluxes were affected by forest management practices, such as forest fire occurrence and suppression, cutting and afforestation. Forest fire occurrence generally decreased soil N2O flux, and increased soil CH4 absorption. Forest fires could enhance, reduce or show no effects on soil CO2 flux, depending on fire type, fire intensity and ecosystem type. Forest cutting usually increased soil CO2, N2O and CH4 emission. Afforestation might reduce soil CO2 emissions, where asits effects on N2O and CH4 varied with ecosystem type, planted tree species, etc. Generally, atmospheric change impacts that may affect forest soil GHG fluxes are elevated atmospheric CO2, nitrogen deposition, and rising temperatures. Elevated atmospheric CO2 increased soil CO2 and N2O emission, and decreased soil CH4 absorption. Nitrogen deposition stimulated soil N2O emissions, and suppressed soil CH4 absorption. Rising temperatures increased soil CO2 and N2O emissions. The combined effects of forest management practices and atmospheric change on forest soil GHG fluxes may be non-additive, and effective forest management may alter the response of soil GHG fluxes to atmospheric change.
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    Cloning and stress response of the JrERF2⁃2 gene from Juglans regia
    LIU Yumei, ZHAO Huanyuan, CUI Maokai, WANG Tianyu, GAO Xiangqian, YANG Guiyan
    JOURNAL OF NANJING FORESTRY UNIVERSITY    2020, 44 (3): 58-64.   DOI: 10.3969/j.issn.1000-2006.201909031
    Abstract2515)   HTML92)    PDF(pc) (2879KB)(10080)       Save
    Objective

    Walnut (Juglans regia) is an important woody oil stratey and poverty allevintion tree species in China. Whose growth and development can be affected by adverse environmental conditions such as low temperature, drought or high salt concentrations. However, adaptation mechanisms of walnut trees to such conditions are not comprehensively understood, which limits optimization of management strategies in the walnut industry in terms of improving production quantity and quality. In order to investigate walnut resistance to stress, candidate genes associated with stress resistance were screened in the present study.

    Method

    The Ethylene Response Factor (ERF) was used to screen members of the ERF transcription factor family in the transcriptome of ‘Xiangling’ walnut. Following homology alignments, open reading frame (ORF) confirmation, sequence basic characteristics analyses, and PCR-based vector cloning, an ERF termed JrERF2?2 was selected for further analyses.

    Result

    Using the ExPASy bioinformatics portal, prediction analyses suggested a 906 bp ORF of the JrERF2?2 gene that encodes a protein consisting of 262 amino acids; the mole?cular weight of this protein is 74.43 ku, and the theoretical isoelectric point is 5.07. CD?Search showed an AP2 domain in the JrERF2?2 protein. BLASTP and MEGA analyses suggested considerable similarity of the JrERF2?2 protein with ERF proteins of Betula platyphylla and Quercus suber, indicating potentially similar functions of JrERF2?2 and its homologs. Moreover, to investigate potential adaption to adverse stimuli, a 1 455 bp upstream promoter was identified in the walnut genome, and cis-acting elements were predicted using New PLACE analyses. The results showed that this promoter segment included a variety of stress-related cis-acting elements such as the drought early response element (ACGTATERD1), heat stress response element (CCAATBOX1), and low temperature response element (LTRE1HVBLT49), suggesting that this promoter may regulate JrERF2?2 expression during stress responses. Therefore, to further investigate the role of JrERF2?2 in stress response mechanisms, 2-year-old grafted ‘Xiangling’ walnut seedlings were subjected to salt, drought and abscisic acid (ABA) stress. JrERF2?2 expression was quantified using reverse-transcription quantitative PCR (qRT-PCR) analysis. Salt treatment was applied using 0.3 mol/L sodium chloride (NaCl) for 0, 3, 12 and 24 h; drought stress was elicited using 100 g/kg PEG6000 for 0, 3, 4 and 5 days, and the ABA treatment was applied using 0.1 mmol/L ABA for 0, 3, 24 and 48 h. Total RNA was isolated from leaf samples using a cetyltrimethylammonium bromide (CTAB) method. RNA was then reverse-transcribed to cDNA which was diluted 10-fold for use as a qRT-PCR template. Reverse transcription was performed using a PrimeScriptTM RT Reagent Kit (CWBIO, Kangwei Century, China), and the reaction was incubated at 42 ℃ for 60 min and at 85 ℃ for 5 s. A SYBR Green Real-time PCR Master Mix and the PCR primers JrERF2-2-F (5′-TGTCACCGAAGTTCCGGAT-3′) and JrERF2-2-R (5′-GATGCAGCTTCTCTAGTC-3′) were used for qRT-PCR. Walnut 18S rRNA (HE574850) was used as an internal reference. Relative expression le?vels were recorded using a 2-ΔΔCt method. The qRT-PCR results showed that JrERF2?2 expression was induced by the NaCl, PEG6000 and ABA treatments, at different transcription levels. Under NaCl stress, JrERF2?2 expression levels increased over time. Relative expression at 12 and 24 h was 2.68- and 6.70-fold higher, respectively, than that at 3 h. Under exposure to PEG6000, JrERF2?2 expression also increased continuously over time with up to 4.12-fold increased expression compared to the control. In the ABA treatment, JrERF2?2 expression was similar to that under NaCl stress, and maximum values observed at 48 h were 6.57-fold higher than those at 3 h. NaCl, drought and ABA treatment results indicated that JrERF2?2 expression can be induced by these stressors.

    Conclusion

    Thus, JrERF2?2 is an important member of the ERF family and may have similar functions to homologs in other species. Its upstream promoter contains many cis-elements associated with stress responses, suggesting that this promoter can effectively regulate JrERF2?2 expression in response to stress. JrERF2?2 expression can be induced by salt, drought and ABA treatments, to varying degrees, and the expression patterns shared certain similarities, indicating that JrERF2?2 can respond to drought- and salt-induced osmotic stress. Moreover, JrERF2?2 may be involved in the ABA signaling pathway. Taken together, JrERF2?2 is an important candidate gene for revealing the mechanism of walnut adaptation to adverse conditions.

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    Advance on miR160/miR167/miR390 family and its target genes in plants
    YANG Chunxia, XU Meng, WANG Mingxiu, HUANG Minren
    JOURNAL OF NANJING FORESTRY UNIVERSITY    2014, 38 (03): 155-159.   DOI: 10.3969/j.issn.1000-2006.2014.03.030
    Abstract6428)      PDF(pc) (1406834KB)(9003)       Save
    MicroRNA(miRNA)is endogenous and non-coding small RNA with 21-23 nucleotides, and it widely exits in eukaryotes. MiRNAs repress gene expression at post transcriptional level by acting on near-perfect complementary sequences in mRNA coding region to guide cleavage and translational repression, and it plays an important role in regulating cell cycle and development timing of biology. Three miRNA families including miR160, miR167 and miR390 all targeting to ARF(Auxin response factor)gene family have been reviewed, and which indicates that they fulfil the similar and differential function. For example, miR160/miR167/miR390 plays an important role in plant development. But miR160 focuses on regulating embryo and root development, miR167 focuses on the regulation of flowers and fruit development, and miR390 has remarkable effect on regulating plant lateral organ development. Moreover, feedback regulation exists between miR160/miR167/miR390 and their target genes.
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    Taxonomic and geographic differentiation of phylogenetic conservatism of plant functional traits: a case study of maximum plant height of Chinese angiosperms
    XING Bingbing, LI Yao, MAO Lingfeng
    JOURNAL OF NANJING FORESTRY UNIVERSITY    2024, 48 (1): 59-66.   DOI: 10.12302/j.issn.1000-2006.202204044
    Abstract2776)   HTML39)    PDF(pc) (3438KB)(8219)       Save

    【Objective】 Plant height is a fundamental component of plant ecological strategies. This study aims to elucidate the phylogenetic conservatism of maximum plant height in Chinese angiosperms, investigate its variation across taxonomic groups and geographic regions, and explore its associations with environmental factors. 【Method】 Using data on the maximum plant height and geographic distribution of 20 295 Chinese angiosperm species, this study assessed the phylogenetic conservatism of maximum plant height among different growth forms, taxonomic groups, and vegetation zones. The study analyzed the correlation between the phylogenetic signal (Pagel’s λ) and factors such as latitude, temperature, and precipitation. 【Result】 The maximum plant height of Chinese angiosperms exhibited a strong phylogenetic conservatism (Pagel’s λ = 0.893). Among them, herbaceous plants showed slightly lower phylogenetic conservatism compared to woody plants, while six orders including Fabales exhibited phylogenetic signals higher than 0.9. The phylogenetic signals of all plants and woody plants displayed a unimodal relationship with latitude and temperature but showed a negative correlation with precipitation. The latitudinal pattern of phylogenetic signals in herbaceous plants was less pronounced, instead showing a multi-peak relationship with temperature and precipitation. 【Conclusion】 The maximum plant height of Chinese angiosperms demonstrated a clear geographic pattern in terms of phylogenetic conservatism, but the trend varied among different growth forms. Overall, the phylogenetic conservatism of maximum plant height of angiosperms and woody plants was stronger in middle latitudes compared to both high and low latitudes.

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    Dynamic changes of nutrients during flora development in heterodichogamous Cyclocarya paliurus
    HUANG Peng, MAO Xia, HAN Ge, CHEN Xiaolin, FU Xiangxiang
    JOURNAL OF NANJING FORESTRY UNIVERSITY    2018, 42 (05): 1-9.   DOI: 10.3969/j.issn.1000-2006.201802018
    Abstract6720)      PDF(pc) (2593848KB)(8160)       Save
    【Objective】Cyclocarya paliurus is a typical heterodichogamous species. To demonstrate nutrient the regulatory mechanism of heterodichogamous characteristics of flowering in C. paliurus, dynamic changing patterns of nutrients in the flowers and surrounding tissues during various developmental stages of the floral were monitored. 【Method】 In two mating types(protandry, PA and protogyny, PG)of C. paliurus selected from a plantation, changes in morphology and length of male and female inflorescences(male, ♂; female, ♀)were observed and measured; also, dynamic changes in starch, soluble sugar, and soluble protein content in the flowers and surrounding tissues(branch surrounding male bud,♂Z; branch and leaves surrounding female bud,♀Z and♀Y)during the process of floral development were measured. 【Result】 ① Starch: much starch was found in the flower buds and branches before initiation, and the contents declined with the process of inflorescence development. The content of starch in♂Z was significantly higher than in♀Z, and the content in♂Z of PA was higher than that of PG. ②Soluble sugar: large amounts of soluble sugar were found in♂Z before bud initiation and changing patterns of soluble sugar showed a dynamic pattern of “declining to increasing, then a tendency to stabilize.” The content in the♂fluctuated with a little change, whereas the tendency of “rising to declining” and the maximum in the end of elongation growth of the ♀ occurred in the flower and surrounding tissues. ③ Soluble protein: more soluble protein was accumulated in the♂of PA than PG before bud initiation, and the content decreased gradually with floral development; whereas the content in♂Z changed a little. Soluble protein in the♀reached a peak before elongation growth of the inflorescence; whereas changing patterns of the content in♀Y of PA were observed. However, changing patterns of the soluble protein content in♀Z were different between PA and PG. 【Conclusion】Soluble sugar for floral development was from soluble sugar and hydrolysis products of starch stored in flowers and surrounding the branch, whereas soluble sugar stored in♂Z of PA was much higher than that of PG. Soluble protein stored in the♂or photosynthetic products input provided the needs for male flower development, whereas photosynthetic products were the only source for female flower development. Although changing patterns of starch and soluble nutrients are different in stages and sequences of male and female flowers between PA and PG, they were synchronized precisely with the process of floral development.
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    Research advances and molecular mechanism on SPL transcription factors in regulating plant flower development
    TIAN Jing, ZHAO Xueyuan, XIE Longsheng, QUAN Jinyi, YAO Lianmei, WANG Guodong, ZHENG Yaoqang, LIU Xuemei
    JOURNAL OF NANJING FORESTRY UNIVERSITY    2018, 42 (03): 159-166.   DOI: 10.3969/j.issn.1000-2006.201708015
    Abstract6739)      PDF(pc) (1538677KB)(8070)       Save
    Abstract: SPL(squamosa promoter-binding protein-like)transcription factor is a kind of gene family unique to plants. It is widely found in green plants and plays an important role in plant growth and development. Flower development is the most important process in plant reproductive development, including changes in the different developmental patterns, such as flowering determination, flower evocation, and floral organ development. This paper summarized the structure and function of SPL transcriptional factors, and in particular described the molecular mechanism and biological function of the SPL gene during plant flower development. Finally, we concluded that SPL transcription factors can be directly or indirectly involved in the photoperiod pathway, gibberellin pathway, and age pathway to control the flowering time in plants. The SPL gene can directly activate the downstream floral meristem identity genes,such as LEAFY(LFY), which regulate the flowering transition in plants. The SPL gene can regulate floral organ and fertility development by the interaction of downstream floral organ identity genes, such as controlling the length and shape of the inflorescence and pedicel, and the size of floral organ. The SPL gene can also regulate plant microsporogenesis and megasporogenesis, male and female gametophyte development. According to related research results with Arabidopsis, we have preliminarily mapped the molecular mechanism of Arabidopsis flowering regulation.
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    Advances in developmental biology of bamboos
    DING Yulong, LIN Shuyan, WEI Qiang, YAO Wenjing, QUE Feng, LI Long
    JOURNAL OF NANJING FORESTRY UNIVERSITY    2022, 46 (6): 23-40.   DOI: 10.12302/j.issn.1000-2006.202208067
    Abstract1449)   HTML66)    PDF(pc) (1881KB)(8058)       Save

    Although the history of bamboo resources utilization can be traced back to 7 000 years ago, the understanding of bamboo as a special group of Gramineae was not established until the late 18th century. Carl von Liné, the founder of modern plant classification, treated all bamboo species in the world as one species belonging to the genus Arundo (Arundo arbor) in his book “Species Plantarum”. It was not until 1789 that bamboo was separated from Arundo and the first bamboo genus, Bambusa, was established. Since its recognition as a distinct species from other grasses, there has been a wealth of literature addressing these aspects, including morphology, anatomy, systematics, physiology, ecology, cytology, genetics and molecular biology. However, systematic research on the occurrence, development and senescence of various organs of bamboo plants is still lacking. This article reviews and summarizes research on the developmental biology of the bamboo root, rhizome, culm, leaves and reproductive organs. We highlight what is insufficient or lacking in the field of bamboo developmental biology and what should be further investigated. We proposed to consider the entire process of cell division, differentiation and morphogenesis when studying the developmental biology of bamboo, and to investigate the dynamics of physiological and biochemical indices throughout the process to reveal the gene expression and signal transduction pathways that control each step of the entire developmental process.

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    Cited:
    STUDY ON RELATIONSHIP BETWEEN FIRST-ORDER BRANCH CHARACTERISTICS AND GROWTH TRAITS, STEM FORMFOR POPULUS DELTOIDES×POPULUSEURAMERICANH F_1 CLONES
    Li Huogen;Huang Minren;Wan Minxiu
    JOURNAL OF NANJING FORESTRY UNIVERSITY    1994, 18 (01): 1-7.   DOI: 10.3969/j.jssn.1000-2006.1994.01.001
    Abstract3017)      PDF(pc) (158465KB)(7533)       Save
    First-order branch characteristics,growth traits and stem form of populus hybrids(1-69*I-45)F1 clones were researched by divided the crown into three parts.Greater variation among clones for growth and branch traits are found. analysis on correlation and path shows that the ideal branch structure of I-69*I-45 F1 clones is:branch angle,branch lenth and branch number gradully increase from upper crown to down crown,less little branch in the upper and middle crown,more in the down crown. In the end of this poper,three types of branch structures ate classified.
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    THE USE OF THE CALCULATION OF THE SOLAR APPARENT COORDINATE TO REFORM THE HEIGHT OF AN OBJECT SURVEY AND POSITIONS
    Lin Genshi
    JOURNAL OF NANJING FORESTRY UNIVERSITY    1991, 15 (03): 89-93.   DOI: 10.3969/j.jssn.1000-2006.1991.03.018
    Abstract4435)      PDF(pc) (246897KB)(7303)       Save
    Based on spherical astronomy, this paper deals with the establishment of a simple mathematical model, and complicated calculation of solar apparent coordinate, and it is also concerned with a simple convenient multifunction program designed and developed by the calculation of the solar apparent coordinate suitable for a computer or program calculator on the basis of general engineering requirement and survey theory. The use of the calculating procedure can perform calculation of the geographical coordinate and direct and invert calculation of the shadow’ s length and the height of an object. This method has the advantage of both convenience and exactness and the practical value as well.
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    Study on the Osmanthus fragrans blooming season phenology
    DONG Lige, WANG Xianrong, DING Yulong
    JOURNAL OF NANJING FORESTRY UNIVERSITY    2014, 38 (增刊): 51-56.   DOI: 10.3969/j.issn.1000-2006.2014.S1.011
    Abstract4087)      PDF(pc) (2085604KB)(7204)       Save
    Through three years observation of Nanjing area a few autumn sweet Osmanthus cultivars blooming season phenology,compared their blooming season phenology and the external morphological characteristics of the flower bud or flower. At the same time, analyzes of the relationship between phenology and temperature, think that after the completion of flower bud differentiation and development to some extent, and daily minimum temperature below critical temperature is the key to enter the florescence. That performance for the ball-shaped flower bud expansion immediately, and then enter to top bracts stage; during the stage of top bracts to blossom stage is not strict requirements on low temperature. In the six research cultivars, the florescence of Osmanthus fragrans ‘Zaoyingui’ is the earliest, then O. fragrans ‘Zaoziyin’ and O. fragrans ‘Xionghuang’ research florescence,the florescence of the large number of blossom of O. fragrans ‘Wanyingui’, O. fragrans ‘Jinqiugui’ and O. fragrans ‘Jinshi’ at the latest.
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    A review on the studies of molecular interaction between forest trees and phytopathogens
    TIAN Chengming, WANG Xiaolian, YU Lu, HAN Zhu
    JOURNAL OF NANJING FORESTRY UNIVERSITY    2021, 45 (1): 1-12.   DOI: 10.12302/j.issn.1000-2006.202010001
    Abstract1280)   HTML976)    PDF(pc) (1791KB)(7082)       Save

    In recent years, great achivements have been made on the study of the molecular interaction between forest trees and phytopathogens. Particularly, thanks to the novel technology including HIGS and CRISPR/Cas9, there are many breakthroughs towards the functional analysis of key virulence-related genes, pathogen genome and transcriptome analysis, pathogenic effector protein analysis, functional analysis of R gene in forest trees, the balance between disease resistance and growth and molecular breeding for disease control. This paper starts with the basic issue of molecular plant-pathogen interaction currently and reviews the current progress and hotspots of molecular interaction between forest trees and pathogens, including the signal network of pathogen invasion, the detoxification mechanism of reactive oxygen species and the multiple action mechanisms of effector proteins, the research progress of omics between trees and pathogens, the key defense mechanism of trees, the interaction mechanism between trees and endophytic fungi and exophytic fungi, and so on. Based on the current research progress, we prospect the future development trend of tree-pathogen molecular interaction. At the same time, the emergence of new theories such as the Zigzag model and Decoy hypothesis, new technologies such as efficient sequencing and molecular manipulation, and new research directions such as effector protein and immune receptor interaction indicate a whole new stage of forest trees and pathogen interactions.

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    Observation of morphological and anatomical characters on staminate and pistillate flower differentiation in Carya illinoensis(Wangenh.)C. Koch
    HAN Minghui, PENG Fangren, DENG Qiuju, TAN Pengpeng
    JOURNAL OF NANJING FORESTRY UNIVERSITY    2017, 41 (06): 1-7.   DOI: 10.3969/j.issn.1000-2006.201703020
    Abstract6239)      PDF(pc) (2977093KB)(6488)       Save
    【Objective】 The demand for the pecan cultivar ‘Pawnee'(pro)is high in China. However, its heterodicogamous often cases unstable yield. Studying the process of flower differentiation is essential for the adjustment of the flowering period and balance year's production. 【Method】We combined phonological observation with photographs of paraffin sections to study the developmental progress during flower differentiation; we observed ‘Pawnee''s(protandrous)fruiting bud differentiation and the growth of female and male gametophytes. 【Result】We established a connection between external morphology and internal anatomy during development. Male pecan flower budding initiates in early April; this is then followed by the inflorescence differentiation stage, stamen differentiation stage, sepal primordium differentiation stage, stamen growth stage, anther develop stage, and pollen sac and pollen formation stage. The corresponding morphological changes included: male flower buds changed from green and soft to brown and hard, the buds swelled, male flower bud scales cracked, inflorescence elongated, flower bracts swelled and cracking, then anther turn to yellow. The female flower buds began to develop from a terminal branch since mid-April. They then entered the critical period of morphological differentiation as follows: pistillate inflorescence differentiation stage, flower stalk and flower primordium differentiation phase, perianth primordium stage, bract primordium differentiation phase, sepal differentiation stage, pistil differentiation stage, and ovule differentiation stage. The corresponding morphological changes included: female flower bud scales changed from yellow green to brown to gray green, the scales gradually opened, flake off epidermis, floret primordium appeared swollen, exposing the stigma and style of elongation. Thus, it is feasible to judge the period of pecan flower differentiation based on flower buds' morphology and anatomical structure preliminary.
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    A study on somatic embryogenesis in loblolly pine and
    application of several patent media
    WU Li-jun , YE Jian-ren
    JOURNAL OF NANJING FORESTRY UNIVERSITY    2008, 32 (01): 1-.   DOI: 10.3969/j.jssn.1000-2006.2008.01.001
    Abstract9319)      PDF(pc) (750856KB)(6087)       Save
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    JOURNAL OF NANJING FORESTRY UNIVERSITY    2016, 40 (05): 0-.  
    Abstract5397)      PDF(pc) (338317KB)(6042)       Save
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    A review on the sensitivity of soil respiration to temperature
    TANG Yan-fei, WANG Guo-bing, RUAN Hong-hua
    JOURNAL OF NANJING FORESTRY UNIVERSITY    2008, 32 (01): 128-.   DOI: 10.3969/j.jssn.1000-2006.2008.01.031
    Abstract10050)      PDF(pc) (884069KB)(6026)       Save
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    Phytoremediation mechanisms of air formaldehyde pollution
    XIONG Yanfei, CHEN Yuefeng, MAO Zhiqiang, CAO Xinghong, YE Yuhan, ZHANG Jiankun
    JOURNAL OF NANJING FORESTRY UNIVERSITY    2023, 47 (4): 1-12.   DOI: 10.12302/j.issn.1000-2006.202111029
    Abstract1217)   HTML47)    PDF(pc) (1835KB)(5992)       Save

    Phytoremediation is the most energy-saving, environmentally friendly, and effective method for the air formaldehyde removal based on the physiological and metabolic characteristics and environmental adaptability of plants. The formaldehyde removal efficiencies of many indoor ornamental plants and some small wild plants, crops and genetically modified plants have been investigated. Plant formaldehyde scavenging involves three stages: absorption, transport and metabolism. Formaldehyde is absorbed by plants mainly through the leaves (stomata, guard cells, and leaf surface cuticle diffusion) and root hair. Most of the formaldehyde absorbed by plants was metabolized in tissue cells at the absorption site, and a small portion is transported to the rhizosphere or from the roots to the leaves. Formaldehyde is metabolized primarily through the Calvin cycle, C1 metabolism and glyoxylate pathway. Different metabolic pathways have distinct roles and characteristics. Based on this, the following suggestions are proposed:(1) Development of plant species with a strong formaldehyde-scavenging ability. (2) Exploring new ways for plants to metabolize formaldehyde. (3) It is necessary to make a profound study on the effects of formaldehyde metabolisms on normal photosynthesis and biomass production in plants.

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    The poplar genome project and progress in poplar molecular biology studies
    CHENG Qiang, PAN Huixin,XU Lian, ZHUGE Qiang,WANG Mingxiu*,HUANG Minren*
    JOURNAL OF NANJING FORESTRY UNIVERSITY    2009, 33 (01): 131-135.   DOI: 10.3969/j.jssn.1000-2006.2009.01.028
    Abstract9515)      PDF(pc) (742762KB)(5877)       Save
    Forest trees as renewable resources are economically important which possess treespecific characteristics comparing to herbaceous plants such as extensive secondary growth, seasonal growth and dormancy, longgeneration times. Studies on the molecular mechanism underlying treespecific traits have facilitated our understanding of plant biology and have potential value for genetic engineering. Poplar has been chosen as the model species for woody plants and the poplar wholegenome has been sequenced successfully. Meanwhile, profound studies have been carried out on poplar transcriptome and proteome. Poplar genome project has enriched our knowledge of tree genome evolution, wood formation, flowering, dormancy and treepathogen interactions.
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    TabZIP transferred Betula platyphylla generation and salt tolerance analysis
    LI Yuanyuan,YANG Guang,WEI Rui,SUN Yanshuang,GUO Yuhua, ZHANG Ruiping,LIU Guifeng*
    JOURNAL OF NANJING FORESTRY UNIVERSITY    2013, 37 (05): 6-12.   DOI: 10.3969/j.issn.1000-2006.2013.05.002
    Abstract4046)      PDF(pc) (1935985KB)(5872)       Save
    Plant basic leucine zipper (bZIP) proteins was an important group of plant transcription factors, as these proteins could interact with cisacting ABRE elements. These proteins activated the expression of some resistance related genes such as drought and salt tolerant genes. In order to create novel salttolerant transgenic lines, the transcription factor gene TabZIP was imported from Tamarix into the birch genome by using agrobacterium mediated transfection. Six transgenic lines were confirmed by qRTPCR and Western blotting. We compared the relative height growth rate, chlorophyll fluorescence parameters and salt injury index in transgenic lines before and after salt stress treatments. The results showed that the TB4 transgenic line was superior to other transgenic lines after salt stress treatments, with a relatively higher height growth(524%), increased by 73.5% if compared to control. The salt damage of TB4 transgenic gene was the lowest, with a salt injury index of 005, while controls were 019. After salt treatment, TB4 demonstrated different chlorophyll fluorescence parameters from the other transgenic lines, with the highest increase in the nonphotochemical quenching parameter, the lowest decrease in the actual photochemical efficiency of PSⅡ and the photochemical quenching coefficient, and the maximal quantum yield of PSⅡ. The changes in these indicators demonstrated that the expression of exogenous TabZIP could improve the resistance of plants to salt stress, and of these transgenic lines obtained would lay foundation for further research into the resistance mechanism of TabZIP.
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    JOURNAL OF NANJING FORESTRY UNIVERSITY    2002, 26 (03): 97-97.   DOI: 10.3969/j.jssn.1000-2006.2002.03.027
    Abstract1569)      PDF(pc) (9238277KB)(5762)       Save
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    Cloning and function analysis of PnsGA20ox1 gene in Populus simonii ×P. nigra
    ZHANG Lijie,ZHANG Kaixuan,WEI Zhigang
    JOURNAL OF NANJING FORESTRY UNIVERSITY    2013, 37 (06): 11-16.   DOI: 10.3969/j.issn.1000-2006.2013.06.003
    Abstract6546)      PDF(pc) (1716603KB)(5706)       Save
    The gene fragment of GA20ox was cloned from Populus simonii×P. nigra by using degenerate primers based on the conservative of GA20ox gene homologous genes. The full-length of cDNA was isolated by using RACE.The cDNA was 1 678 bp and encoded a protein of 385 amino acids with Mr= 44. 04 ku and pI=5.97.The nucleotides shared 99% identity with the GA20ox1 gene of Populus trichocarpa.It was named PnsGA20ox1. Quantitative real-time PCR analysis showed the PnsGA20ox1 gene expressed in all organs of Populus simonii×P.nigra, and the expression level of PnsGA20ox1 was the highest in mature xylem but the least in internode organizations.The transgenic tobacco showed that the holocellulose content and fiber length in overexpression of the PnsGA20ox1 gene of tobacco improved comparised with the control,while there was no significant difference in lignin content comparised with the control.
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    Study on the surface dynamic wettability and free energy of poplar
    ZHANG Yang,ZHOU Zhao-bing,YUAN Shao-fei,PAN Hui-xin
    JOURNAL OF NANJING FORESTRY UNIVERSITY    2008, 32 (01): 53-.   DOI: 10.3969/j.jssn.1000-2006.2008.01.011
    Abstract11944)      PDF(pc) (387310KB)(5660)       Save
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    Physiological-biochemical changes? in seedlings of different Toona sinensis?provenances under drought stress
    YANG Yu-zhen,PENG Fang-ren*,CEN Xian-chao,PENG Cheng
    JOURNAL OF NANJING FORESTRY UNIVERSITY    2008, 32 (01): 28-.   DOI: 10.3969/j.jssn.1000-2006.2008.01.006
    Abstract10141)      PDF(pc) (965626KB)(5630)       Save
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    The current scenario and sustainable developmental strategies for genetically improved tree seed production bases in China
    WANG Zhangrong
    JOURNAL OF NANJING FORESTRY UNIVERSITY    2020, 44 (5): 1-8.   DOI: 10.3969/j.issn.1000-2006.202005016
    Abstract1723)   HTML548)    PDF(pc) (1426KB)(5604)       Save

    The year 1964 was remarkable and historical time in terms of the construction of large-scale genetic improvement programs and improved tree seed production bases in China. Seed orchards of Chinese fir (Cunninghamia lanceolata) at the state-owned Yangkou Forest Farm, Fujian Province; slash pine (Pinus elliottii) in Hongling, Taishan, Guangdong Province; and Mongol scotch pine (Pinus sylvestris var. mongolica) at Qingshan Forest Farm, Linkou, Heilongjiang Province, were established. Over half a century later, there great progress was noted. A total of 99 national tree species germplasm banks and 294 national key improved seed productive bases (seed orchards and breeding arboretums) were established, and 170 million kilograms of improved seeds and over 280 billion nursery stocks were produced. Moreover, 3 224 improved tree varieties were tested and approved, and the supplement of genetically improved seeds covered 65% of the new afforestation area. The achievement of genetic improvement was also satisfactory. For instance, the genetic gains for volume growth of 6-year-old 1 st, 1.5 th and 2 nd generation offspring of Chinese fir clone seed orchards in Zhejiang Province were 16.97%, 22.58% and 26.42%, respectively. The genetic gains were up to 32.82% and 37.93% from the original and bi-clone Chinese fir seed orchards, respectively, and the genetic gains for volume growth of the 3 rd generation off spring of Chinese fir clone seed orchard in Fujian Province reached 76%. In the 21 st century, the structure of tree species for constructing forest breeding bases was adjusted. Owing to the abundant resources of tree species in China (there are over 2 000 tree species, many of which serve special purposes and specific functions), the management and direction of improvement of breeding bases in China are highly diversified. In addition to the breeding of good varieties of common industrial wood species such as Pinus, Cunninghamia and Larix, the varieties of the following tress were genetically improved: Chinese fir, masson pine (Pinus massoniana), Chinese pine (Pinus tabulaeformis). special wood species such as fragrant rosewood (Dalbergia odorifera), Nanmu (Phoebe bournei), and Manchur ash (Fraxinus mandshurica); naturally resistant tree species such as saxoul (Haloxylon ammodensron), sandthorn (Hippophae rhamnoides), and diversiform-leaved poplar (Populus euphratica); landscape tree species such as tulip tree hybrids (Liriodendron sino-americanum), Yulan magnolia (Magnolia denudata), and golden larch (Pseudolarix amabilis); oil and spice species such as oil-tea tree (Camellia oleifera), oil tung (Vernicia fordii), and star anise (Illicium verum); dry fruit tree species such as hickory (Carya cathayensis), Chinese torreya (Torreya grandis), and Chinese dates (Ziziphus jujuba); and medicinal species such as Tu-Chung (Eucommia ulmoides), officinal magnolia (Magnolia officinalis), and dogwood (Cornus officinalis), among others. The construction of forest breeding bases is a basic project to realize the modernization of forestry in China, and it is the key step in the construction of forest seed programs. In the future, strategic measures for sustainable development of forest breeding bases in China will include the following six steps: ① stabilizing the long-term economic subsidy investment and ensuring long-term normal operation of the base; ② strengthening the operational political management and supervision as well as formulating and revising technical standards and regulations; ③ strengthening scientific and technological supports and improving the coordination of the “Three combinations”(taking the breeding base of improved varieties of production units as the platform, the administrative leading departments of forest seedling management as the leading, and the scientific and technological teams of key disciplines or key laboratories of teaching or scientific research institutions as the technical support team); ④ developing long-term tree breeding programs to ensure the continuation of tree genetic improvement; ⑤ setting up a management steering committee and establishing a scientific decision-making mechanism; and ⑥ regularly holding breeding seminars to discuss the key issues in development.

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    Structure, function and mechanisms of bacterial protein Hfq
    FAN Ben, CHEN Sheng, LI Yulong
    JOURNAL OF NANJING FORESTRY UNIVERSITY    2016, 40 (05): 155-162.   DOI: 10.3969/j.issn.1000-2006.2016.05.025
    Abstract5886)      PDF(pc) (1725366KB)(5572)       Save
    Bacterial small RNAs(sRNAs)have been one of the important research subjects in microbiology in last decade. They mainly act as a group of regulators controlling bacterial gene expression at post-transcriptional level. Most sRNAs regulate their mRNA targets involved by Hfq, which can facilitate base-paring of sRNA-mRNA and further affect mRNA stability or translation process. In this work the structure of Hfq, the molecular mechanisms of Hfq in sRNA regulation, and its influences detected in various bacterial species are reviewed. As a small chaperon protein ubiquitously present in many bacteria, the homohexamer form of Hfq possesses two unequivocal facets which can bind different types RNAs and promote their interaction. Such a structure provides a basis for its functional roles in mediating sRNA-mRNA interaction. While the hitherto studies on Hfq are mainly restricted in Gram-negative bacteria, its roles in some Gram-positive bacteria remain elusive. Moreover, the mechanisms underlying the impacts of Hfq on many important bacterial species have been still not clear. In future, it is necessary to further investigate the fine structure of Hfq with regard to its function as well as the detailed mechanism of the diverse bacterial phenotype affected by Hfq.
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    Genetic variations of open-pollinated progenies from the second seed
    orchard and selection of superior families of Chinese fir
    WENG Yu-zhen
    JOURNAL OF NANJING FORESTRY UNIVERSITY    2008, 32 (01): 19-.   DOI: 10.3969/j.jssn.1000-2006.2008.01.004
    Abstract8328)      PDF(pc) (700642KB)(5462)       Save
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