Most Download

Published in last 1 year | In last 2 years| In last 3 years| All| Most Downloaded in Recent Month | Most Downloaded in Recent Year|

In last 2 years

Please wait a minute...


For Selected: Download Citations EndNote Ris BibTeX Toggle Thumbnails

Select

Advances in research of wildland-urban interface fires WANG Qiuhua, WANG Jin, LI Xiaona, MA Cheng, HONG Ruicheng, CAO Hengmao, GAO Zhongliang Journal of Nanjing Forestry University (Natural Sciences Edition）    2024, 48 (5): 1-10.   DOI: 10.12302/j.issn.1000-2006.202303047 Abstract （5338）   HTML （2667）    PDF（pc） （1599KB）（5412）       Knowledge map    Save As the wildland-urban interface (WUI) becomes an increasingly important area of focus, the close relationship between human settlements and natural ecosystems such as forests has led to a high incidence of fires. Human activities play significant roles in the occurrence of fires in the WUI, and with rapid urbanization and the construction of forest cities, the complexity and severity of these fires have increased. This paper provides an overview of research progress on fires in the WUI, through literature analysis and case studies. At present, the concept of the WUI in China lacks clarity and standardization, highlighting the need for further research in this area. Future research should focus on expanding the database of interface fire cases and integrating satellite monitoring systems, video surveillance, and big data platforms to establish an early warning and monitoring system for the WUI. In addition, strengthening the research on fire behavior simulation and remote sensing monitoring, especially UAV fire behavior detection technology research on fire behavior simulations and remote sensing monitoring of WUI and unmanned aerial, remote-sensing monitoring and other new technologies will be crucial for providing effective solutions for fire prevention and control in the WUI. This will also contribute to the improvement of management measures, standards, and risk reduction strategies. Overall, the emphasis on new technologies and scientific research will provide a solid foundation for the management and reduction of fire risks in the WUI. Table and Figures | Reference | Supplementary Material | Related Articles | Metrics | Comments（0） Cited:

Select

Affecting factors analysis of functional diversity at different forest strata in an old growth forest community in Yachang Natural Reserve WANG Yaoyi, WANG Hongxiang, WANG Yongqiang, ZENG Wenhao, YE Shaoming Journal of Nanjing Forestry University (Natural Sciences Edition）    2024, 48 (5): 28-38.   DOI: 10.12302/j.issn.1000-2006.202208030 Abstract （2220）   HTML （471）    PDF（pc） （2573KB）（3971）       Knowledge map    Save 【Objective】 Functional diversity is crucial for forest biodiversity protection and maintenance of ecosystem stability. Exploring the characteristics and causes of functional diversity variation at different stratum levels will assist in understanding the maintenance mechanisms of biodiversity and plant community assembly processes. 【Method】 Leaf traits and environmental factors were collected from forest plot data from an 1.6 hm2 evergreen broadleaved forest in Guangxi Yachang Orchidaceae National Natural Reserve. K-means partitioning and Gaussian kernel density estimation were used for stratification. The direct and indirect effects of species diversity and environmental factors (light, topography, and soil) on functional diversity were explored in different forest strata using structural equation models. 【Result】 The diversity indices of the understory were greater than those of the canopy (P<0.05). Species diversity had a significant direct impact on all four functional diversity indices in the understory layer, whereas only the functional richness index (FRic) and quadratic entropy (RAOQ) indices were affected directly by species diversity in the canopy layer (P<0.05). A significant indirect effect (β = 0.278, P<0.01) of canopy openness on FRic index and a direct effect (β = -0.593, P<0.01) of canopy openness on functional evenness index (FEve) were found for understory trees. Topographic factors affected canopy functional diversity. Specifically, there was a relatively significant direct effect of aspect on FEve (β = -0.420, P<0.01) and RAOQ (β = -0.300, P<0.05) indices for canopy trees. Soil factors mainly affected the functional diversity of different forest layers through species diversity. Soil total phosphorus, available phosphorus, alkaline hydrolysis nitrogen, and available potassium content had a greater effect on the functional diversity of the understory, whereas soil total potassium content affected the FRic and RAOQ indices of the canopy. 【Conclusion】 There were marked differences in the characteristics of functional diversity at different forest stratum in the study area. Functional diversity was mainly affected by species diversity, canopy openness, and soil nutrients of understory plants, whereas topographic factors determined the functional diversity of the canopy layer. This study reveals the effects of biological and abiotic environmental factors on functional diversity across different forest layers and expands the understanding of the diversity of vertical stratification. The findings provide a further reference for biodiversity protection in natural forests. Table and Figures | Reference | Related Articles | Metrics | Comments（0） Cited:

Select

A comprehensive evaluation of fruit characteristics of 46 blueberry cultivars ZHAO Huifang, ZHAO Fengyi, LIU Hongxia, WU Wenlong, WU Yaqiong, LI Weilin Journal of Nanjing Forestry University (Natural Sciences Edition）    2025, 49 (3): 52-62.   DOI: 10.12302/j.issn.1000-2006.202408010 Abstract （4608）   HTML （3608）    PDF（pc） （2145KB）（3596）       Knowledge map    Save 【Objective】This research aims to evaluate the fruit characteristics of 46 blueberry cultivars for selection and breeding purposes. 【Method】The fruit appearance, inner quality and sensory properties of 46 blueberry cultivars were investigated, then classified and evaluated by correlation, principal component and cluster analyses. 【Result】The northern highbush blueberry exhibited the largest fruit size, highest and acidity levels, and achieved the highest sensory score. The fruit of southern highbush blueberry was the smallest, with the lowest hardness and sensory score, the highest sweetness and total polyphenol content. The fruit of rabbit eye blueberry was larger than southern highbush blueberry, with the highest pericarp ratio and total anthocyanin content. Among appearance indices, the chromaticity a* of fruit with epicuticular powder removed exhibited the highest coefficient of variation (CV) at 2 926.91%, followed closely by the original fruit chromaticity a* at 370.96%. The CV of solid acid ratio (42.77%) was the highest among quality indices, followed by total anthocyanin content (39.78%), and the CV of sensory score was the lowest (<10%). Correlation analysis revealed that larger fruit size was associated with lower pericarp percentage and total polyphenol content. The acidity had the greatest effect on the solid acid ratio of blueberry fruits. Sensory evaluation indices, including appearance and comprehensive scores, showed significant positive correlations with fruit weight, transverse diameter, and longitudinal diameter, indicating that larger fruit size is a preferred characteristic in sensory evaluation. Cluster analysis showed that the 46 blueberry cultivars were divided into three types, among which the first type had the largest fruit with high hardness and acidity, the second type had the second-largest fruit with the highest sweetness, and the third type had the smallest fruit with high pericarp ratio, belonging to the processing type with high total anthocyanin and polyphenol content. Principal component analysis reduced the 11 appearance and quality indices to four principal components, explaining 75.217% of the total variance. Sensory evaluation revealed that, in addition to differences in size, sweet-sour balance and nutrient content, the 46 blueberry cultivars also varied in wax thickness, texture and fruity aroma, and seven large-fruit cultivars of ‘Primadonna’, ‘Millennia’, ‘Jewel’, ‘Chandler’, ‘Brigitta’, ‘Aurora’ and ‘Prolific’, 3 high sweetness cultivars of ‘Anna’ ‘Sweetheart’ and ‘Brightwell’, and four excellent taste cultivars of ‘Sunshine’, ‘Magnolia’, ‘Brigitta’ and ‘Beckyblue’ were selected. Comprehensive evaluation showed that sensory assessment results were largely consistent with principal component and cluster analyses. 【Conclusion】The 10 cultivars with large fruit size, high sweetness, crisp texture and rich aroma were ‘Prolific’, ‘Chandler’, ‘Sweetheart’, ‘Brigitta’, ‘Millennia’, ‘Beckyblue’, ‘Primadonna’, ‘Brigitta’, ‘Magnolia’ and ‘Pink Lemonade’, which were recommended for fresh consumption, and the 5 cultivars with high total anthocyanins and polyphenols content and high pericarp percent were ‘Gardenblue’, ‘Zhongzhi 4’, ‘Zhaixuan 4’, ‘Baldwin’ and ‘Centurion’, which can be used for processing blueberry products. These cultivars can also serve as parental materials for corresponding breeding objectives. Table and Figures | Reference | Supplementary Material | Related Articles | Metrics | Comments（0） Cited:

Select

Analyzing the genetic diversity of Nymphaea spp. based on SSR markers MAO Liyan, LI Huimin, LONG Lingyun, HUANG Qiuwei, TANG Yuwei, YU Yanping, HUANG Xinyi, TAN Xiaohui, NONG Xiaohui, ZHU Tianlong, LU Zushuang Journal of Nanjing Forestry University (Natural Sciences Edition）    2024, 48 (5): 57-68.   DOI: 10.12302/j.issn.1000-2006.202302029 Abstract （2456）   HTML （421）    PDF（pc） （2779KB）（3141）       Knowledge map    Save 【Objective】Nymphaea spp. (waterlily) are important aquatic flowers. The rapid development of the waterlily industry has led to challenges in identifying genetic backgrounds of newly introduced germplasms in a timely manner due to inadequate introduction management practices. This has resulted in issues such as mislabeling of seedlings and unclear parentage, hampering the effective utilization and innovation of waterlily germplasm resources. This study focuses on developing genome-wide SSR markers for conducting phylogenetic and genetic diversity analyses of waterlily species. These markers are intended to serve as a theoretical foundation for conserving and breeding waterlily germplasm resources.【Method】Based on the published complete genome sequence of waterlily, the SSR loci in 14 chromosome genes were analyzed using the micro satellite identification (MISA) tool. Subsequently, 150 pairs of SSR primers were designed with the assistance of Primer 3.0 software. Five native germplasms were chosen for PCR amplification utilizing the 150 primer pairs. Following PCR, SSR primers demonstrating high polymorphism were identified through agarose and denaturing polyacrylamide gel electrophoresis. The selected SSR primers were synthesized with fluorescent primers (FAM and HEX) before amplifying 147 waterlily samples via PCR. The resulting products were then assessed using capillary electrophoresis, and the raw data were analyzed using GeneMarker software. Fragment sizes at each allele site for every sample were compiled into a 0/1 matrix. Genetic diversity indices, cluster analysis, and principal component analysis were computed using Popgenen and NTSYS software toos.【Result】11 pairs of SSR primers exhibiting distinct bands and high polymorphism were carefully chosen following the analysis of agarose gel and polyacrylamide gel electrophoresis results. These selected primers were employed to evaluate the genetic diversity among 147 waterlilies. Capillary electrophoresis revealed the presence of 307 alleles. The polymorphism index (PIC) ranged from 0.46 to 0.60, average at 0.53. The effective allele number (Ne) rangedfrom 1.042 8 to 1.117 5, with an average of 1.071 8. Nei’s gene diversity index (H) ranged from 0.038 0 to 0.086 2, averaging at 0.056 2. The Shannon diversity information index (I) ranged from 0.085 6 to 0.163 8, averaging at 0.114 4. Cluster analysis indicated genetic similarity coefficients ranging from 0.781 8 to 0.993 5 among the 147 waterlilies, with an average coefficiency of 0.899 2. These waterlilies were classified into six groups based on a genetic similarity coefficient was 0.879 0. Principal coordinates analysis (PCoA) displayed results closely aligned with traditional morphological classification for the 147 waterlilies. The first, second and third principal coordinates accounted for 16.54%, 8.35% and 5.43% of the total genetic variation, respectively, comprising 30.32% altogether. The first coordinate was correlated with aroma formation and stamen development, the second with flowering time and environmental adaptability, and the third with flower types.【Conclusion】11 pairs of SSR primers displaying substantial polymorphism were chosen, demonstrating efficacy in distinguishing the genetic relationships among 147 waterlilies. These waterlilies were systematically categorized into six primary branches, with classification outcomes mirroring traditional morphology categorizations. The selected 11 pairs of SSR primers have potential utility in analyzing genetic diversity and identifying waterlily species. The SSR markers findings stand poised to offer a robust scientific foundation for germplasm collection, preservation, innovation, and the development of waterlily species. Table and Figures | Reference | Related Articles | Metrics | Comments（0） Cited:

Select

Conservation status and prioritization of rare and endangered plants in Jiangxi Wuyishan National Nature Reserve CHEN Lin, CAO Xiaomeng, PAN Tingting, LEI Ping, ZENG Lijian, LI Meng, YI Xiangui Journal of Nanjing Forestry University (Natural Sciences Edition）    2024, 48 (5): 39-47.   DOI: 10.12302/j.issn.1000-2006.202212017 Abstract （4482）   HTML （1947）    PDF（pc） （1499KB）（2954）       Knowledge map    Save 【Objective】This research aims to determine the actual threat level and prioritize the conservation of rare and endangered plants in the Wuyishan National Nature Reserve in Jiangxi Province, provide a reference for the conservation of these plants in the nature reserve.【Method】Using the line transect method combined with historical literature, the endangered coefficient, genetic value coefficient, and species value coefficient were used to quantitatively analyze the conservation priorities of 113 rare and endangered wild plants in the Wuyishan National Nature Reserve in Jiangxi Province. The relationship between the rare and endangered plants of Wuyishan in Jiangxi Province and five adjacent areas, namely Wuyishan in Fujian Province, Tianmushan in Zhejiang Province, Lushan in Jiangxi Province, Wanchaoshan in Hubei Province, and Jinfoshan in Chongqing City, were compared using the Sorensen similarity coefficient.【Result】(1) There were 113 species of rare and endangered plants in the reserve, belonging to 47 families and 85 genera. Among these, there were 6 species classified as National Class Ⅰ, 15 species as National Class Ⅱ. 20 species were classified as Jiangxi Province Class Ⅰ, 11 species as Jiangxi Province Class Ⅱ, 55 species as Jiangxi Province Class Ⅲ, and 12 species endemic to Jiangxi. (2) According to the quantitative analysis of conservation priority, 17 species (15.04%) were assessed as first class, 41 species (36.28%) as second class, 54 species (47.79%) as third class, and 1 species (0.89%) as fourth class. (3) The similarity coefficient between Wuyishan in Jiangxi Province and the five adjacent areas were about 30%. However, Wuyishan in Jiangxi Province owned more rare and endangered plants (38 species) and a higher proportion of species per unit area (0.24), indicated a higher conservation value.【Conclusion】 The excellent habitats and abundant rare and endangered plants in Wuyishan in Jiangxi Province indicate a high conservation value. The assessment and classification of the 113 rare and endangered plants more accurately reflect their current situation in the Nature Reserve, providing a reference for effective conservation plans for threatened species. Table and Figures | Reference | Related Articles | Metrics | Comments（0） Cited:

Select

Analysis of genetic diversity and construction of core collections of Korean pine (Pinus koraiensis) natural population YAN Pingyu, ZHANG Lei, WANG Jiaxing, FENG Kele, WANG Haohao, ZHANG Hanguo Journal of Nanjing Forestry University (Natural Sciences Edition）    2024, 48 (5): 69-80.   DOI: 10.12302/j.issn.1000-2006.202304035 Abstract （2674）   HTML （423）    PDF（pc） （2522KB）（2429）       Knowledge map    Save 【Objective】 Korean pine (Pinus koraiensis) is a valuable tree species that is distributed throughout northeastern China. Over the past century, human interference has led to a gradual decrease in the number of individuals and distribution of its natural population. Assessing the genetic diversity and building a core collection of natural Korean pine could provide a scientific basis for the effective conservation, management, and utilization of Korean pine germplasm resources. 【Method】 A total of five well-preserved natural populations of Korean pine in Hebei, Wuying, Xiaobeihu and Jixi in Heilongjiang Province and Lushuihe in Jilin Province in northeast China were studied. A combination of phenotypic data and molecular markers was used to construct the core collection. 【Result】 Molecular and phenotypic ANOVA results showed that the genetic variation of Korean pine natural populations mainly originated from inter-individual differences, which accounted for 96% and 72.84% of the total variation, respectively. The Jixi population was genetically distant from other populations, with an average Fst of 0.026 8. It also had a high genetic diversity, with Shannon and phenotypic diversity index values of 1.111 and 2.00, respectively. The population structure analysis showed that the five Korean pine natural populations had no obvious subpopulation structure. There were no significant changes in the genetic diversity of Korean pine populations among the different forest ages. Additionally, in the younger forest there was no evidence of heterozygous deletions or inbreeding. There was a broad correlation between needle traits and geographic factors, resulting in the phenotypic differentiation of Korean pine populations. 【Conclusion】 The Shannon and phenotypic diversity indexes of the core collection constructed by combining molecular and phenotypic markers with a 30% sampling ratio were 1.076 and 2.018, respectively, which was representative of the genetic status of Korean pine populations. This information can be used to better manage the germplasm resources of Korean pine and promote its protection and use. The genetic structure characteristics indicated a need to focus on in situ protection of the natural germplasm and to promote ecological recovery, germplasm protection, and use of Korean pine. Table and Figures | Reference | Related Articles | Metrics | Comments（0） Cited:

Select

Cloning of the Liriodendron chinense LcPIN1a genes and its effect on plant growth and development HAO Zhaodong, MA Xiaoxiao, WANG Dandan, LU Ye, SHI Jisen, CHEN Jinhui Journal of Nanjing Forestry University (Natural Sciences Edition）    2024, 48 (6): 51-61.   DOI: 10.12302/j.issn.1000-2006.202404005 Abstract （2035）   HTML （1104）    PDF（pc） （5246KB）（2415）       Knowledge map    Save 【Objective】This study aimed to explore the role of the auxin transporter PIN1 in plant growth and development in Liriodendron chinense.【Method】Three PIN1 homologous proteins were identified in the Liriodendron genome using bioinformatic methods, and expression pattern analyses of the three LcPIN1s genes were performed in different tissues and response to various abiotic stresses. An overexpression vector driven by the CaMV35S promoter was then constructed and transformed into Arabidopsis and Liriodendron×sinoamericanum, followed by phenotypic determination of growth and developmental traits in the transgenic positive lines.【Result】Three PIN1 homologous proteins were identified in the Liriodendron genome, named LcPIN1a, LcPIN1b and LcPIN1c. Expression pattern analyses showed that LcPIN1a was mainly expressed in leaves, while LcPIN1b and LcPIN1c were primarily expressed in roots and stems and stigmas when the plantlets transitioned into reproductive growth. In addition, all three LcPIN1 genes transcriptionally responded to drought stress, with LcPIN1b and LcPIN1c showing dependence on the biosynthesis of endogenous ABA, while LcPIN1a does not. Root length and plant height were significantly reduced in LcPIN1a-heterologous overexpression (LcPIN1a-HO) lines compared to wild-type Arabidopsis. The number of stamens was predominantly five in LcPIN1a-HO lines, whereas wild-type Arabidopsis typically contained six stamens. The regeneration of plantlets in LcPIN1a-overexpressing (LcPIN1a-OE) Liriodendron×sinoamericanum was significantly reduced compared to wild-type plants. In addition, the root length and plant height of LcPIN1a-OE regenerated seedlings were significantly lower than those of the wild type. The root structure of LcPIN1a-OE plants was significantly changed, with the taproot being less distinct.【Conclusion】The PIN1 proteins of L. chinense play a crucial role in vegetative and reproductive growth. Overexpression of LcPIN1 genes can be detrimental to normal plant growth and development. Table and Figures | Reference | Related Articles | Metrics | Comments（0） Cited:

Select

Catalogue of Chinese gymnosperms based on the most recent gymnosperm classification system LI Meng, ZHOU Yexi, YANG Yong Journal of Nanjing Forestry University (Natural Sciences Edition）    2024, 48 (4): 49-56.   DOI: 10.12302/j.issn.1000-2006.202405016 Abstract （4163）   HTML （1301）    PDF（pc） （1427KB）（2147）       Knowledge map    Save 【Objective】Professor Wan-Chun Cheng conducted the first systematic classification study of gymnosperms in China. In 1978, the results were published in Volume 7 of the Flora Republicae Popularis Sinicae, introducing a novel classification system for gymnosperms in the country. This seminal work systematically organized gymnosperm species in China, establishing a strong foundation for subsequent research in gymnosperm classification in the region.【Method】Building on the latest gymnosperm classification system proposed by Professor Yang Yong in 2022, this study compiled a new species checklist of gymnosperms in China. Data from Volume 7 of the Flora Republicae Popularis Sinicae (FRPS), Flora of China Vol. 4 (FOC), and the Catalogue of Life in China 2023 were reviewed, along with recent relevant literature.【Result】The results revealed that China is currently home to 267 wild gymnosperm species, distributed among 37 genera within nine families (including 27 infraspecific taxa). We propose two new combinations: Sabina carinata (Y.F. Yu & L.K. Fu) Y. Yang and Meng Li and Sabina arenaria (E.H.Wilson) W.C. Cheng and W.T. Wang ex Y. Yang and Meng Li.【Conclusion】This study enhances the species catalog of wild gymnosperms in China, serving as a crucial reference for further botanical classification research and conservation efforts aimed at native gymnosperms. Table and Figures | Reference | Supplementary Material | Related Articles | Metrics | Comments（0） Cited:

Select

Carbon dynamic simulation based on Biome-BGC model in mixed coniferous and broadleaved forest of Fengyang Mountain, Zhejiang Province HUANG Luyao, DU Shanfeng, JI Xiaofang, GUAN Xin, LIU Shenglong, YE Limin, JIANG Jiang Journal of Nanjing Forestry University (Natural Sciences Edition）    2024, 48 (5): 11-20.   DOI: 10.12302/j.issn.1000-2006.202211005 Abstract （2363）   HTML （619）    PDF（pc） （4196KB）（1891）       Knowledge map    Save 【Objective】This study aims to investigate the carbon dynamics of subtropical mixed coniferous and broadleaved forests in Fengyang Mountain, Zhejiang Province and their response to climate change. 【Method】The Biome-BGC model was used to simulate the net primary productivity (NPP), gross primary productivity (GPP), and net ecosystem productivity (NEP) in Fengyang Mountain from 1979 to 2018, to investigate the relationships between climate factors and NPP at different time scales. Pearson correlation analysis and quadratic function fitting were performed between climate factors and NPP at different temporal scales to explore the relationship and response patterns between NPP and major climate factors, and finally, different climate scenarios were applied to predict the carbon cycling trends in Fengyang Mountain in the next 100 years. 【Result】The average values of GPP, NPP and NEP of mixed coniferous and broadleaved forests in Fengyang Mountain for 40 years were 1 392.94, 451.25 and 16.21 g/(m2·a), respectively. Except for 1984, 2002, 2005, 2008 and 2010, which were carbon sinks and showed that the sensitivity of NPP to temperature change was the highest, and the increase of temperature in summer had a positive effect on the increase of NPP, while the increase of temperature in winter had a negative effect on NPP. To a certain extent, winter rainfall showed a positive effect on NPP, while summer precipitation showed a negative effect on NPP. The gross primary productivity of Fengyang Mountain forests in RCP2.6, RCP4.5 and RCP6.0 scenarios will keep increasing in the 21st century, and by 2100, the GPP of the studied forests in Fengyang Mountain under RCP2.6, RCP4.5 and RCP6.0 scenarios will reach 1 552.73, 1 660.30 and 1 960.41 g/(m2·a), respectively, and get increased 1.38%, 8.41% and 28.00% relative to the GPP in 2018. 【Conclusion】Overall, the forest ecosystem of Fengyang Mountain exhibited carbon sinks under normal conditions, but the cloudy and rainy summer weather in the mountainous area inhibited the increasing effect of temperature on carbon sinks to some extent. The future warming, increased rainfall and higher CO2 concentration simultaneously will favor the vegetation growth of mixed coniferous forests in Fengyang Mountain. Table and Figures | Reference | Related Articles | Metrics | Comments（0） Cited:

Select

Precise genomic editing technology and its application in the improvement of woody plants JIANG Bo, AN Xinmin Journal of Nanjing Forestry University (Natural Sciences Edition）    2025, 49 (1): 11-20.   DOI: 10.12302/j.issn.1000-2006.202402006 Abstract （2074）   HTML （424）    PDF（pc） （1995KB）（1818）       Knowledge map    Save The rapid advancement of genome precision editing technologies has revolutionized our understanding of life’s mysteries. Central to these technologies is the capacity to precisely insert, delete, and substitute DNA sequences at designated genomic positions, facilitating the targeted modification of genetic information within living organisms. These technologies have become the cornerstone of research in the field of biology, from early explorations to the groundbreaking application of the CRISPR system, emphasizing the profound impact of scientific inquiry. The application of the CRISPR system has brought about a significant leap in genomic editing, catalyzing the emergence of more precise editing tools, such as base editors and prime editors, which have significantly enhanced the ability to edit genomes with precision. This milestone shift has already demonstrated tremendous potential in a wide range of fields, including agricultural improvement and disease treatment. The potential applications for gene editing technology are vast, especially in forestry and grass breeding. The CRISPR/Cas system can knock out multiple genes, offering high targeting efficiency, ease of design and operation, and low costs, making it widely applicable in crop genetic improvement. However, it is also associated with numerous limitations, which can be overcomed by new editing tools. As the technologies continues to advance, gene editing technology is expected to solve complex biological problems in the future, bringing more innovation and breakthroughs to human health and agricultural development. Table and Figures | Reference | Related Articles | Metrics | Comments（0） Cited:

Select

Research progress on the dust retention effect and physiological-ecological response of Osmanthus fragrans YANG Jianxin, GUO Shuailong, MA Changle, LI Rui, GAO Can, KANG Xinling, LI Fulong Journal of Nanjing Forestry University (Natural Sciences Edition）    2025, 49 (2): 1-11.   DOI: 10.12302/j.issn.1000-2006.202308013 Abstract （1920）   HTML （947）    PDF（pc） （1519KB）（1621）       Knowledge map    Save Vegetation effectively removes airborne particulate matter through “direct” and “indirect” pathways, each with distinct mechanisms and processes. Osmanthus fragrans, one of the top ten traditional flowers in China, is widely distributed in south of the Qinling Mountains across the subtropical regions. Research results indicate that this species plays a significant role in mitigating atmospheric particulate pollution and improving air quality by retaining particulate matter. We analyzed and summarized advancements in understanding the dust retention capacity of O. fragrans, emphasizing key quantitative indicators such as dust retention per unit leaf area, per single leaf, and the ability to retain particulates of various sizes. This study also explored spatiotemporal variations in dust retention and its impact on spectral characteristics, morphological structure, and physiological ecology. Differences in dust retention among O. fragrans varieties and habitats were attributed to genetic traits and environmental factors, including air pollution levels, leaf morphology, leaf area, phyllotaxy, inclination angle and crown shape. Key findings include the following:(1) Correlation with environmental pollution. Dust retention per unit leaf area was positively correlated with the background level of air pollution. (2) Influence of morphological traits: dust retention per single leaf was determined by leaf morphology and environmental conditions, though no significant correlation was found between single-leaf area and particulate retention capacity. (3) Plant-level variation. Total dust retention varied significantly among plants due to differences in leaf area across growth stages, health conditions, and locations. (4) Particulate size specificity. Leaves retained coarse particulates more effectively than fine ones, and dust retention showed predictable spatiotemporal variation. Diurnal and seasonal trends aligned with fluctuations in airborne particulate matter, with species- and region-specific saturation times. (5) Impact on leaf traits. Particulate retention altered leaf spectral characteristics, affecting morphology, functional traits, and adsorption capacity. These changes influenced physiological ecology, growth, floral fragrance, and ornamental value. Table and Figures | Reference | Related Articles | Metrics | Comments（0）

Select

Distribution patterns of ant species in southeastern Yunnan Province YANG Lin, ZHU Huiqin, XU Zhenghui, ZHANG Xinmin, ZHOU Xueying, XU Guolian, LIU Xia Journal of Nanjing Forestry University (Natural Sciences Edition）    2024, 48 (5): 204-210.   DOI: 10.12302/j.issn.1000-2006.202211035 Abstract （2815）   HTML （1038）    PDF（pc） （2570KB）（1585）       Knowledge map    Save 【Objective】The study aimed to investigate the patterns and laws of ant species distribution, including the habitat, vertical distribution, foraging patterns and nesting sites in southeastern Yunnan Province, provide a scientific basis for biodiversity cataloging and protection in this area.【Method】The distribution patterns of ant species in southeastern Yunnan Province were investigated using the plot-sampling method, and the data were counted using the harvesting frequency method. The Pearson correlation between the altitude and species richness of the ants was analyzed using the SPSS 26 software. 【Result】 A total of 197 species of ants, belonging to 64 genera and nine subfamilies under the Formicidae family, were recorded from five vertical zones in southeastern Yunnan Province. The majority of ant species inhabited different vegetation zones; however, a few ants, including Aphaenogaster schurri and Lasius himalayanus, inhabited only a single vegetation type. Of the 13 habitats, the ants (87 species) were most abundant in the middle-and low-altitude monsoon evergreen broadleaf forest, and least abundant (eight species) in the high-altitude Quercus semicarpifolia forest. The majority of ant species had a narrow ecological adaptation range, and the height difference in vertical distribution was less than 500 m. A few species of ants, including Ectomomyrmex javana, Nylanderia flavipes, and others exhibited a wide ecological adaptation range, and the height difference in vertical distribution was greater than 2 000 m. There were differences in the foraging zones and nesting sites of the majority of ant species. A few species, including Crematogaster osakensis, Formica cunicularia and Dolichoderus taprobanae, foraged and nested in multiple sites, and the species that foraged on the ground and nested in the soil were the most abundant. Correlation analysis revealed that the altitude and species richness were significantly negatively correlated at 0.01 level of significance. 【Conclusion】There are abundant resource for ant populations in southeastern Yunnan Province, and the distribution patterns of ant species in the region are primarily related to the altitude, types of vegetation, and adaptability. The species richness was found to be high in the middle-and low-altitude areas, which have a hot and humid climate, rich vegetation types, and sufficient food resources and nesting sites; however, the ants in the high-altitude areas inhabited fewer habitats and their species richness was low due to the cold climate and single type of vegetation. The study revealed that the functional characteristics of the ants determine their viability. The majority of ant species exhibited a strict environmental selection, and there were obvious differences in the distribution patterns of different species. Table and Figures | Reference | Supplementary Material | Related Articles | Metrics | Comments（0） Cited:

Select

Effects of volatile organic components of Ilex rotunda on its insect pollinator, Apis cerana KUANG Zeyu, PENG Ye, FANG Yanming Journal of Nanjing Forestry University (Natural Sciences Edition）    2024, 48 (4): 254-260.   DOI: 10.12302/j.issn.1000-2006.202211014 Abstract （2349）   HTML （1039）    PDF（pc） （2099KB）（1566）       Knowledge map    Save 【Objective】The relationship between odorous substances in flowers and the visiting behavior of insects has attracted increasing attention from biologists. However, previous studies on specific plants and pollinators are scare. The present study investigated the species differences and dynamic changes in the volatile organic components of male and female flowers of Ilex rotunda during flowering, and recorded the species and behaviors of pollinators. The study aimed to elucidate the characteristics of the pollinating system, explore the potential effects of volatile compounds on the behaviors of pollinating insects, and provide a reference for improving the pollination and fruiting of I. rotunda.【Method】The changes in the volatile components during the four flowering stages, the big bud stage, first blooming stage, full blooming stage, and final flowering stage, were determined by head space solid phase microextraction (HS-SPME) and gas chromatography-mass spectrometry (GC-MS). The species, visiting behavior, and visiting frequency of the pollinators were recorded by observation.【Result】The results demonstrated that the flowering of I. rotunda began in late April and ended in early May in Nanjing, with a flowering period of nearly half a month. A total of 30 volatile substances were detected from the flowers, of which 19 and 21 were detected from female and male flowers, respectively. There was a moderate difference in the overall number of male and female plants. The results revealed that the levels of β-caryophyllene and other major components increased initially, reached a peak at the full-opening stage, and decreased thereafter. The proportion of the main components generally decreased at the final flowering stage. However, there was an increase in the proportion of alcohols and alkanes during final flowering, which was accompanied by the production of several new compounds. The sole insect pollinator of I. rotunda is Apis cerana. There was a considerable overlap between male and female plants during the peak visiting period. The findings revealed that A. cerana preferred visiting male flowers, and that there was a positive correlation between the changes in the visiting frequency pattern and β-caryophyllene content.【Conclusion】The results indicated that β-caryophyllene could be used as an information substance to attract A. cerana for pollinating I. rotunda. Additionally, male and female I. rotunda plants can be planted in a centralized and reasonable manner according to the “centralized” flower visiting behavior of A. cerana. The success rate of I. rotunda pollination can be effectively improved by complying with the consistent flowering period, the concentrated flowering period, and planting large numbers of male plants. Table and Figures | Reference | Related Articles | Metrics | Comments（0） Cited:

Select

An analysis of the disturbance mechanism of tourist destinations at the edge of world geological parks based on the MSOR theoretical model WANG Haoyi, ZHANG Jianguo, LYU Yuanwan Journal of Nanjing Forestry University (Natural Sciences Edition）    2025, 49 (3): 227-234.   DOI: 10.12302/j.issn.1000-2006.202311042 Abstract （2178）   HTML （1766）    PDF（pc） （1598KB）（1527）       Knowledge map    Save 【Objective】This study was conducted to explore the impact mechanisms of different tourism motivations on the tourism experience, satisfaction, and loyalty of tourists in the edge tourist destinations of Yandang Mountain Global Geopark, providing theoretical support and practical insights for understanding the disturbance mechanisms of edge tourist destinations. 【Method】Amos and Mplus software were employed to administer a survey questionnaire targeting the consumption behavior characteristics of tourists in the non-core scenic area of the Yandang Mountain Global Geopark, specifically the South Yandang Mountain. 【Result】(1) The primary tourist group in peripheral tourist destinations consists of local residents, predominantly core families. (2) Noelty motivation exhibited no direct or indirect effects on loyalty. (3) Relationship strengthening’s influence on loyalty was fully mediated, demonstrating positive effects on tourism experience and loyalty through mediating effects. (4) Isolation motivation and escape motivation exhibited both direct and indirect positive effects on loyalty, with complex and multiple chain-mediated impacts. These findings reveal the impact mechanisms of different tourism motivations on tourist experiences, satisfaction, and loyalty in edge tourism destinations. Local tourists were identified as playing a crucial role in the protection and sustainable development of edge tourism destinations. 【Conclusion】The study suggests that scenic spots should avoid large-scale construction, leverage customer characteristics to mitigate disturbance risks, and cautiously supplement tourist demand to prevent disturbances from expanding. Table and Figures | Reference | Related Articles | Metrics | Comments（0）

Select

Tree species identification of combined TLS date and UAV images ZHONG Hao, WANG Chuhong, LIN Wenshu Journal of Nanjing Forestry University (Natural Sciences Edition）    2024, 48 (4): 104-112.   DOI: 10.12302/j.issn.1000-2006.202205041 Abstract （2709）   HTML （509）    PDF（pc） （3331KB）（1510）       Knowledge map    Save 【Objective】Rapid and accurate identification of tree species is crucial for the research and protection of forest resources. Identification of tree species by remote sensing technology has become an important method of forest investigation. However, there are some problems in tree species identification by remote sensing, such as a lack of information in the upper canopy of terrestrial laser scanning (TLS) data and the lower canopy of unmanned aerial vehicle (UAV) images. Therefore, identifying tree species requires multi-source remote sensing data.【Method】In this study, Pinus sylvestris var. mongolica, P. tabuliformis var. mukdensis, Fraxinus mandshurica and Juglans mandshurica in the urban forestry demonstration base of Northeast Forestry University at Harbin were used as the research objects to identify tree species. TLS point cloud data and UAV image data were acquired. Through the processing of UAV images, the photogrammetric point cloud and orthophoto image were obtained. The UAV image point cloud and TLS point cloud data were registered and fused, then divided into single tree points. Based on the single tree point cloud, shape features, structure features, tree trunk color features, and crown color features were extracted, and tree species identification was performed by a support vector machine classification algorithm. Subsequently, the ability of the method to identify tree species using different characteristics was analyzed by random forest algorithm.【Result】Optimal results were obtained when all the features were used to identify tree species. The total accuracy and Kappa coefficient of tree species identification results were 93.48% and 0.91, respectively, which were improved by 4.35-16.31percentage points and 0.06-0.22, respectively, compared with other comparison schemes.【Conclusion】The tree species recognition method based on the fusion of TLS data and UAV image point cloud data proposed in this study can compensate for the lack of information in the upper canopy of TLS point cloud data and the lower canopy of UAV images to a certain extent, and make full use of the rich information contained in multi-source data for tree species recognition. The method can effectively improve the accuracy of tree species identification. Table and Figures | Reference | Related Articles | Metrics | Comments（0）

Select

Construction of molecular ID for Osmanthus fragrans cultivars based on phenotypic traits and single nucleotide polymorphisms (SNPs) WANG Yihan, LIU Jiaojiao, JIN Peiquan, LI Shuqing, WEI Jianfen, GUO Peng, SHANG Fude Journal of Nanjing Forestry University (Natural Sciences Edition）    2024, 48 (4): 12-24.   DOI: 10.12302/j.issn.1000-2006.202405026 Abstract （3810）   HTML （710）    PDF（pc） （5559KB）（1438）       Knowledge map    Save 【Objective】This study selected core genomic single-nucleotide polymorphism (SNP) loci to establish a rapid SNP genotyping method on the KASP platform, and to construct molecular IDs for Osmanthus fragrans cultivars. This study provides a theoretical foundation for identifying, tracing and protecting the intellectual property of O. fragrans cultivars.【Method】Field surveys were conducted to investigate key phenotypic characteristics of O. fragrans cultivars. Following two rounds of rigorous screening, we identified a set of core SNP markers capable of completely distinguishing previously sequenced cultivars. Subsequently, we analyzed the polymorphic information content (PIC) and expected heterozygosity (He) of each SNP locus. Using the genome sequences of ‘Rixianggui’ as a reference, species-specific KASP primers were designed for PCR amplification. Based on the genotyping results, we constructed cultivar DNA fingerprints and assessed the efficiency of core SNP markers for cultivar identification. Molecular IDs for O. fragrans cultivars were established by integrating phenotypic information codes with molecular fingerprint codes.【Result】We retained a total of 14 core SNP loci from genomic SNPs that fully discriminated the sequenced cultivars. The PIC values of these loci ranged from 0.246 to 0.375, with an average of 0.335, and the He indices ranged from 0.288 to 0.500, averaging 0.431. The KASP primers designed for these core SNP loci produced accurate genotyping results, enabling us to construct DNA fingerprints capable of distinguishing all 90 tested cultivars, including those not previously sequenced. Each cultivar was assigned a molecular ID composed of 34 digits.【Conclusion】In conclusion, 14 core SNP loci (SNP1 to SNP14) were identified that effectively discriminate among at least 90 O. fragrans cultivars. Unique molecular ID codes were constructed using DNA fingerprint codes along with serial codes derived from cultivar group types and phenotypic characteristics. Finally, barcode and quick response (QR) codes were generated for each cultivar. Table and Figures | Reference | Supplementary Material | Related Articles | Metrics | Comments（0） Cited:

Select

The bird diversity spatial and temporal distribution patterns and its hotspot areas identification in Chengdu City REN Shichao, ZHANG Yinlong, CAO Mingchang, LIU Wei, QIAO Shufan, ZHU Xiaojing, LUO Kangning Journal of Nanjing Forestry University (Natural Sciences Edition）    2024, 48 (5): 189-196.   DOI: 10.12302/j.issn.1000-2006.202212039 Abstract （2504）   HTML （482）    PDF（pc） （2513KB）（1432）       Knowledge map    Save 【Objective】This study aims to explore the structural composition and spatial distribution patterns of bird communities in Chengdu, Sichuan, China, analyze the differences in bird diversity across various habitats and seasons, and identify bird hotspots to provide a basis for bird diversity protection in Chengdu and similar areas.【Method】In 2020, a survey of bird diversity was conducted across different seasons in 20 administrative districts of Chengdu using the line transect method and the sampling point methods. Species richness, Shannon-Wiener diversity index, Simpson index, Pielou evenness index, and community similarity coefficient were calculated to analyze bird diversity in Chengdu. Additionally, 41 key bird species were selected and categorized into raptorial birds, terrestrial birds, natatorial birds, and wading birds based on their ecological habits. The MaxEnt model was used to predict the potential habitats of key birds and identify bird biodiversity hotspots in Chengdu.【Result】A total of 261 bird species across 17 Orders and 62 Families were identified, including one species under national first-class key protection and 29 species under national second-class key protection. Among these, there are 99 species of resident birds, 61 species of summer migratory birds, 60 species of winter migratory birds, and 41 species of transient birds. The number of bird species and individuals, the Shannon-Wiener diversity index, the Simpson index, and the Pielou evenness index were highest in winter, followed by spring, autumn, and summer. Among the six habitats studied, forests (200 species) and wetlands (169 species) had the highest bird species counts. Urban land and wetlands had the highest number of individual birds and population sizes. Forest habitats exhibited the highest Shannon-Wiener diversity index and Simpson index, while urban land had the highest evenness index. The MaxEnt model predictions showed that the distribution of raptorial birds was significantly influenced by land cover type, distance from lakes, and distance from forests. Terrestrial birds distribution was largely affected by distance from forests, altitude, and settlement density. The distribution of waterbirds, including natatorial birds and wading birds, was heavily influenced by land cover type and distance from lakes. Chengdu’s bird diversity hotspots are primarily located in nature reserves, the Longmen Mountain Range, Chaoyang Lake, Longquan Mountain Forest Park, Sancha Lake, Xinglong Lake, Qinglonghu Wetland Park, and along the Minjiang and Tuojiang Rivers.【Conclusion】The rich bird diversity in Chengdu is closely linked to its natural environment, characterized by extensive forest areas, high spatial heterogeneity, and abundant food sources, making it a crucial habitat for birds. Although wetlands comprise only about 2% of the city’s land area, they rank second to forests in bird richness and should be a focal point in future bird biodiversity conservation efforts. Table and Figures | Reference | Related Articles | Metrics | Comments（0）

Select

Characteristics of fruit set and fruit sensory quality of blueberry hybrids with different ploidy level LI Xueling, LIU Ying, WU Wenlong, LYU Lianfei, ZHANG Chunhong, LI Weilin, CAO Fuliang Journal of Nanjing Forestry University (Natural Sciences Edition）    2025, 49 (3): 41-51.   DOI: 10.12302/j.issn.1000-2006.202404027 Abstract （2236）   HTML （1478）    PDF（pc） （2577KB）（1409）       Knowledge map    Save 【Objective】In this study, 24 hybrids were made using blueberries of three distinct cultivation cultivar including Vaccinium corymbosum ‘O’Neal’, V. ashei ‘Pink Lemonade’ and V. ashei ‘Bonita’ as the male parent and three ploidy types of blueberries as the female parent. We aimed to investigate the differences in fruit setting and fruit sensory quality after pollinating these crosses. The results may provide some clues for proportion of pollination trees for cultivation and selection of breeding parents in hybrid breeding of blueberry. 【Method】Using the fruits from open pollination of the female parents as the control (CK), sensory quality indices of hybrid fruits from different crosses including fruit appearance parameters, contents of carbohydrates, antioxidant substances, and volatile compounds were evaluated.【Result】Hybridization compatibility between the three male parents and the rabbiteye blueberry cultivars ‘Vernon’ and ‘Briteblue’ was relatively low. Higher fruit set rate was solely found in the hybrid crosses with highbush types using ‘O’Neal’ and ‘Bonita’ as the male parents. After crossing with ‘O’Neal’, the single fruit mass of ‘Primadonna’ was decreased with a significant increase in fruit firmness. In addition, the fruit shape index of ‘Primadonna’ fruits pollinated by ‘Bonita’ increased significantly. Seed observatioin indicated that the hybrid crosses between blueberry ‘Pink Lemonade’ and three southern highbush cultivars all showed the relatively high fruit set rate, and hybrid seeds were not found in the hybrid fruits in the other crosses. Notably, the hybrid crosses between three male parents ‘O’Neal’, ‘Pink Lemonade’ and ‘Bonita’, and the rabbiteye type cultivar ‘Climax’ all revealed no seeds in the fruits. In comparison with each naturally open-pollinated female parent, the contents of sugar substances in the hybrid fruits of highbush types cultivars were significantly increased after the pollination of ‘O’Neal’ and ‘Bonita’. Especially, the solidity-acid ratios of the hybrid fruits of ‘Winsor’ and ‘Primadonna’ were extremely high after the pollination of ‘Bonita’. Further detection of aromatic substances demonstrated that there was a total of 59 volatile compounds in the fruits of all hybrid combinations. Although the types and contents of the volatile substances varied among the combinations, three main common substances, including linalool, caryophyllene oxide, and trans-2-hexenal were detected across almost all hybrid combinations. The content of linalool in the hybrid fruits of ‘Sharpblue’ was both significantly increased after pollination of ‘Bonita’ and ‘O’Neal’, while that of ‘Primadonna’ was significantly increased only after pollination by ‘O’Neal’. Espcecially, the content of trans-2-hexenal in the hybrid fruits of ‘Primadonna’ and ‘Sharpblue’ pollinated by ‘O’Neal’ was significantly higher than those of the naturally open-pollinated fruits. The volatile substances of the rabbiteye blueberry cultivar ‘Climax’ increased significantly after pollination by ‘Pink Lemonade’, and the substances of alkene type covered a relatively higher proportion. The content of total phenols and anthocyanins showed a similar trend in pollination combinations of different ploidy, with the highest content of total phenols and VC in the hybrid fruits of ‘Climax’ pollinated by ‘O’Neal’, and the content of anthocyanins in the fruit of ‘Primadonna’ and ‘Climax’ was relatively higher under natural open-pollination. 【Conclusion】Blueberries of different ploidy levels have a certain cross-compatibility for inter-ploidy hybridization, and the seedless fruit can be obtained in the crosses of the rabbiteye derived cultivar ‘Pink Lemonade’ and three southern highbush varieties. The solidity-acid ratios of hybrid fruits of most of the highbush cultivars were significantly increased after crossing with the rabbiteye cultivar ‘Bonita’. Especially, after crossing with the male parent ‘O’Neal’, the hybrid fruits of rabbiteye cultivar ‘Climax’ bore no seeds with the increase in the accumulation of antioxidant substances. Table and Figures | Reference | Supplementary Material | Related Articles | Metrics | Comments（0） Cited:

Select

Advancements in the research of miRNAs associated with plant drought and salt stress responses SONG Zihe, ZHEN Yan Journal of Nanjing Forestry University (Natural Sciences Edition）    2024, 48 (4): 1-11.   DOI: 10.12302/j.issn.1000-2006.202305016 Abstract （4828）   HTML （733）    PDF（pc） （1798KB）（1312）       Knowledge map    Save China is a maritime power with a long coastline and abundant coastal resources. However, this long costline brings vast areas of saline-alkali land. Additionally, global warming has led to more frequent seasonal drought. Under such extreme conditions, the survival rate of most plants is very low. Therefore, research on the molecular mechanisms of drought resistance and salt tolerance is particularly important. Improving the survival rate of plants in arid and saline-alkali areas can bring significant ecological benefits and economic value. Non-coding RNA (ncRNA) is a class of genetic information molecules transcribed from the genome that do not encode proteins. As a major focus of epigenetic research, ncRNAs were once considered the “dark matter” or “transcriptional noise” of the genome. The most well-known type of ncRNA is microRNA (miRNA), a highly conserved class of short non-coding small RNA molecules that are 20-24 nucleotides in length. They regulate the growth and development of organisms by cleaving and degrading target gene transcripts or inhibiting the translation of target genes through complementary base pairing with target sites. With the development of miRNA research methods such as small RNA sequencing and degradome sequencing, an increasing number of miRNAs and their target genes has been reported in animals and plants. Their biological synthesis, processing, maturation, and functional effects have been elucidated. Plant miRNAs complementarily pair with their target genes mostly in the open reading frame, with a full or nearly full complementarity to the target sequences. These characteristics stimulated the rapid development in plant miRNA research, and a large amount of research has revealed important regulatory roles of miRNAs in plant growth, development, and stress responses. This article provides a systematic review of the features, synthesis process, mode of action, and research progress of plant miRNAs in drought and salt resistance. We summarizes the main techniques and strategies for plant miRNA research in recent years, discuss the existing problems and prospects, and reveals the regulatory mechanisms of miRNA in plant drought and salt resistance, providing a basis for generating new varieties of drought and salt resistance. Table and Figures | Reference | Related Articles | Metrics | Comments（0） Cited:

Select

UAV forestry land-cover image segmentation method based on attention mechanism and improved DeepLabV3+ ZHAO Yugang, LIU Wenping, ZHOU Yan, CHEN Riqiang, ZONG Shixiang, LUO Youqing Journal of Nanjing Forestry University (Natural Sciences Edition）    2024, 48 (4): 93-103.   DOI: 10.12302/j.issn.1000-2006.202209055 Abstract （2837）   HTML （533）    PDF（pc） （3918KB）（1298）       Knowledge map    Save 【Objective】This study proposes the feature segmentation method Tree-DeepLab for unmanned aerial vehicle (UAV) forest images, based on an attention mechanism and the DeepLabV3+ semantic segmentation network, to extract the main feature distribution information in forest areas.【Method】First, the forest images were annotated according to feature types from six categories (Platanus orientalis, Ginkgo biloba, Populus sp., grassland, road, and bare ground) to obtain the semantic segmentation datasets. Second, the following improvements were made to the semantic segmentation network: (1) the Xception network, the backbone of the DeepLabV3+ semantic segmentation network, was replaced by ResNeSt101 with a split attention mechanism; (2) the atrous convolutions of different dilation rates in the atrous spatial pyramid pooling were connected using a combination of serial and parallel forms, while the combination of the atrous convolution dilation rates was simultaneously changed; (3) a shallow feature fusion branch was added to the decoder; (4) spatial attention modules were added to the decoder; and (5) efficient channel attention modules were added to the decoder.【Result】Training and testing were performed based on an in-house dataset. The experimental results revealed that the Tree-DeepLab semantic segmentation model had mean pixel accuracy (mPA) and mean intersection over union (mIoU) values of 97.04% and 85.01%, respectively, exceeding those of the original DeepLabV3+ by 4.03 and 14.07 percentage points, respectively, and outperforming U-Net and PSPNet.【Conclusion】The study demonstrates that the Tree-DeepLab semantic segmentation model can effectively segment UAV aerial photography images of forest areas to obtain the distribution information of the main feature types in forest areas. Table and Figures | Reference | Related Articles | Metrics | Comments（0） Cited:

Select

Phylogenetic relationship and evolutionary patterns of Tsuga pollen morphology: a cluster analysis-based study CHEN Zhihan, SHANG Xin, XIE Zin, ZHANG Xin Journal of Nanjing Forestry University (Natural Sciences Edition）    2024, 48 (4): 37-45.   DOI: 10.12302/j.issn.1000-2006.202406025 Abstract （3284）   HTML （480）    PDF（pc） （5207KB）（1290）       Knowledge map    Save 【Objective】To analyze the phylogenetic relationships and evolutional patterns of pollen morphology in Tsuga, we observed and compared the pollen characteristics of all 10 extant species of Tsuga along with pollen fossils.【Method】Pollen morphology was examined using scanning electron microscopy (SEM), and hierarchical cluster analysis (HCA) was conducted using seven quantitative indicators and one qualitative indicator.【Result】Tsuga pollens typically exhibits characteristics of the N1P3C1 type, with a leptoma on the distal face. The equatorial length ranges from 21.20 to 58.00 μm, predominantly lacking sacci but occasionally with echinae saccate forms. The pollen surface is typically warty or sparsely micro-warty, with spines or without. According to the hierarchical cluster analysis, Tsuga could be divided into three categories: ①saccate but lacking echinae type: T. mertensiana and Nothotsuga longibracteata; ②lacking sacci and echinae type: T. caroliniana, T. canadensis and pollen fossil T. sp.2; ③lacking sacci but echinate type: T. heterophylla, T. ulleungensis, T. diversifolia, T. forrestii, T. sieboldii, T. dumosa, T. chinensis and pollen fossil T. sp.1, T. sp.3.【Conclusion】Pollen morphology in Tsuga has evolved from saccate to non-saccate forms, and from lacking spines to possessing spines. These pollen characteristics are closely related to geographic distribution, indicating similarity among pollen from the same regions. The clustering analysis based on pollen morphology largely aligns with molecular phylogenetic trees, offering a method to distinguish extant species and fossils of Tsuga and providing valuable insights for phylogenetic studies of Tsuga. Table and Figures | Reference | Related Articles | Metrics | Comments（0） Cited:

Select

Analysis and comprehensive evaluation of nutrient components in bamboo shoots of different clones of Chimonobambusa utilis BIAN Lili, LIANG Dahong, FAN Meiling, WU Hongyu, ZHOU Binao, YAO Wenjing, WANG Fusheng, DING Yulong, LIN Shuyan Journal of Nanjing Forestry University (Natural Sciences Edition）    2024, 48 (4): 159-167.   DOI: 10.12302/j.issn.1000-2006.202206046 Abstract （2290）   HTML （449）    PDF（pc） （2345KB）（1280）       Knowledge map    Save 【Objective】Differences in nutrient composition and mineral and amino acid contents between the original species (FY) and six clones (F1-F6) of bamboo shoots of Chimonobambusa utilis were compared to provide an important reference for further breeding and planting of C. utilis.【Method】The nutritional composition of bamboo shoots of C. utilis were analyzed by liquid chromatography, and their quality were evaluated by principal component analysis and cluster analysis.【Result】The water content of the seven kinds of bamboo shoots were greater than 90%. The crude protein content ranged from 1.86% to 5.96%, with clone F4 having the highest content. The fat content ranged from 0.38% to 0.68%, with clone F5 having the lowest content. The dietary fiber content ranged from 22.12% to 29.52%, and clone F6 had the highest content. Vc content ranged from 0.170 5 mg/g to 0.396 1 mg/g, with clone F1 having the highest content. The highest content of total sugar was found in FY, whereas the highest contents of fructose and glucose were in clone F3 and F6, respectively. The highest flavonoid content was found in FY (1.39 mg/g). The total acid content was 13.68-21.04 mg/g, and tannin content was 4.55-9.71 mg/g. In mineral elements, clone F4 had a high content of each element, whereas F2 had the opposite. For selenium content, FY,clone F4 and F6 ranked as the top three among the seven kinds of bamboo shoots. Seventeen amino acids were found in all seven kinds of bamboo shoots. The highest total content of amino acids was in clone F1 (14.93 mg/g), and the lowest content was in clone F3 (8.72 mg/g). The highest essential amino acid content was found in clone F2 (34.25%) and the lowest was in clone F1 (30.38%). The total amount of flavor amino acids was highest in clone F1 (14.64 mg/g) and lowest in F3 (8.08 mg/g). Five key indicators (total acid, calcium, dietary fiber, flavonoids, Vc) were extracted by principal component analysis, with a cumulative variance contribution rate of 96.797%. The overall quality ranking was clone F1, FY, clone F5, F4, F6, F2 and F3. Cluster analysis showed that the seven kinds of C. utilis bamboo shoots were clustered into two groups.【Conclusion】Compared with other bamboo species, the quality of bamboo shoots of the original species and clones of C. utilis is excellent. clone F1, FY and clone F5 showed the best performance in the comprehensive nutritional indexes of nutrient composition, followed by clone F4, F6, F2 and F3. Table and Figures | Reference | Supplementary Material | Related Articles | Metrics | Comments（0） Cited:

Select

Prediction of potential suitable regions of Prunus pseudocerasus based on MaxEnt model under climate change FU Chenlong, LI Meng, TIAN Changfen, SONG Yanfeng, YI Xiangui, WANG Xianrong Journal of Nanjing Forestry University (Natural Sciences Edition）    2024, 48 (4): 235-242.   DOI: 10.12302/j.issn.1000-2006.202212007 Abstract （1612）   HTML （304）    PDF（pc） （4527KB）（1263）       Knowledge map    Save 【Objective】Prunus pseudocerasus, one of the oldest cultivated species in China, is the focus of this study which predicts its suitable growth regions currently and in the future. This serves as a reference for understanding its growth habits and enhancing its cultivation.【Method】The MaxEnt model simulated the distribution of potential suitable areas for P. pseudocerasus under four climate scenarios (SSP126,SSP245,SSP370 and SSP585) at different times. This was based on geographic and environmental climate data. Important climatic variables influencing these areas were identified, and changes in potential suitable regions were analyzed.【Result】The accuracy of the predictions was validated using ROC analysis, achieving a high score of 0.936. Key climatic factors included annual precipitation, minimum temperature of the coldest month, and mean temperature of the warmest quarter. Soil factors did not significantly limit the distribution of P. pseudocerasus. Currently, suitable areas are primarily in the eastern, central and southwest China, with highly suitable regions in central and eastern Shandong, and southern Shaanxi. Climate change is expected to reduce these areas while creating new suitable regions in central Xizang and eastern Jilin.【Conclusion】Under future climate change scenarios, the potential suitable areas for P. pseudocerasus will become fragmented and shift towards higher latitudes and altitudes. These findings provide a theoretical basis for the conservation and industrial development of P. pseudocerasus in China. Table and Figures | Reference | Supplementary Material | Related Articles | Metrics | Comments（0） Cited:

Select

Estimation of forest net primary productivity based on sentinel active and passive remote sensing data and canopy height TIAN Chunhong, LI Mingyang, LI Tao, LI Dengpan, TIAN Lei Journal of Nanjing Forestry University (Natural Sciences Edition）    2024, 48 (4): 132-140.   DOI: 10.12302/j.issn.1000-2006.202205014 Abstract （2662）   HTML （502）    PDF（pc） （1801KB）（1206）       Knowledge map    Save 【Objective】Using open source active and passive remote sensing data (Sentinel-1 & -2) combined with forest canopy height can improve the estimation accuracy of forest net primary productivity (NPP), to provide a scientific basis for the formulation of forest precision management stragtegies and a “carbon peaking and carbon neutrality” strategy.【Method】Zixing City, which is a key forest area in the south, was used as the research area. Based on Sentinel-1 and Sentinel-2 active and passive remote sensing data, four models, namely multiple stepwise regression, artificial neural network, K-nearest neighbor, and random forest, were used to estimate NPP. On this basis, the canopy height obtained by Sentinel-1 through the difference between InSAR and SRTM DEM was added to analyze its effect on the accuracy of NPP estimation.【Result】(1) The mean value of forest NPP in the study area in 2019 was 7.79 t/hm2, showing the spatial distribution characteristics of high in the central southwest and low in the northwest. (2) Among the four models, the accuracy of NPP estimation by active and passive remote sensing was higher than that by single remote sensing; the accuracy of random forest estimation of regional forest NPP was the highest, and the model performed the best. (3) Adding canopy height improved the estimation accuracy of forest NPP to a certain extent, with R2 increased from 0.70 to 0.75.【Conclusion】The accuracy of NPP estimation can be improved based on Sentinel active and passive remote sensing data and canopy height factor, which is obtained by DEM difference method. Table and Figures | Reference | Supplementary Material | Related Articles | Metrics | Comments（0） Cited:

Select

The sap flow characteristics and responses to branch and leaf pruning during reproductive phenological periods in Sapindus saponaria WANG Mianzhi, ZHENG Yulin, JIA Liming, LI Lu, LUO Shuijing, LIU Jiming, LIU Juntao Journal of Nanjing Forestry University (Natural Sciences Edition）    2024, 48 (6): 13-22.   DOI: 10.12302/j.issn.1000-2006.202204005 Abstract （1162）   HTML （300）    PDF（pc） （2235KB）（1137）       Knowledge map    Save 【Objective】The present study aimed to investigate the characteristics of sap flow velocity of Sapindus saponaria and its response pattern to pruning and meteorological factors during the key phenological periods, and to provide a theoretical reference for scientific water management during key reproductive phenological periods of Sapindus.【Method】The present study was conducted in the experimental area of the Sapindus National Forest Germplasm Repository in Jianning County, Sanming City, Fujian Province. Using five-year-old S. saponaria ‘Yuanhua’. Thermal dissipation probes (TDPs) and automatic weather stations were utilized to monitor sap flow and meteorological data from April 20, 2021 to November 9, 2021. The probes were installed approximately 30 cm above the ground to ensure that all probes were installed under the first branch. Sample trees in the pruning group were pruned once after inflorescence growth at the inflorescence growth period to remove branches and leaves that shaded the inflorescence from sunlight. Trees in the control group were left unpruned for comparison.【Result】The average daily sap flow velocity of S. saponaria showed a decreasing-increasing-decreasing trend over time during the seven key reproductive phenological periods. The strongest transpiration activities were (1.13±0.05)×10-3 cm/s (pruning) and (1.48±0.05)×10-3 cm/s (CK) in the early ovary growing period, while the weakest transpiration activities were (0.15±0.02)×10-3 cm/s (pruning) and (0.26±0.03)×10-3 cm/s (CK) in the fruit maturity period. Pruning significantly reduced the sap flow velocity of S. saponaria in all phenological periods, except for the bud swelling period. This reduction effect was more pronounced on sunny days when the tree was actively growing and was strongest at the end of the fruit development period. In addition, solar radiation, air temperature, wind speed and vapor pressure deficit (VPD) were the main drivers of sap flow velocity, while precipitation and air humidity were the main limiting factors. Meteorological factors, such as persistent rainfall, were the main limitations of the transpiration activity of S. saponaria from the bud swelling period to the early flowering period and middle fruit development period. Leaf aging and gradual dormancy of the tree were the main reasons for low sap flow velocity during the fruit maturity period.【Conclusion】Pruning significantly reduced the transpiration, with the reduction effect becoming more pronounced as the phenological periods progressed. There were large fluctuations in sap flow velocity throughout the reproductive growth of S. saponaria. Thus, these findings suggested that the water demand for tree and fruit growth is closely related to the water consumption behavior of trees. Meteorological factors, such as persistent excessive rainfall, during inflorescence growth and development are not conducive to transpiration. Table and Figures | Reference | Supplementary Material | Related Articles | Metrics | Comments（0） Cited:

Select

Analysis of SSR locus based on the whole genome sequences of Cyclocarya paliurus and the development of polymorphic primers LIU Li, QU Yinquan, YU Yanhao, WANG Qian, FU Xiangxiang Journal of Nanjing Forestry University (Natural Sciences Edition）    2024, 48 (4): 67-75.   DOI: 10.12302/j.issn.1000-2006.202206001 Abstract （3453）   HTML （1204）    PDF（pc） （2807KB）（1127）       Knowledge map    Save 【Objective】Genomic simple repeat sequence (SSR) loci were analyzed by screening the whole genome of Cyclocarya paliurus. DNA molecular ID cards of 19 excellent medicinal clones of C. paliurus were constructed based on the newly-developed SSR primers. These genomic SSR markers could support further research, such as the evaluation of the germplasm resource, analysis of genetic diversity, and identification of cultivars/clones.【Method】The SSR loci were screened along with the whole genome of C. paliurus and were enriched and analyzed using MISA software. Subsequently, SSR primers were designed using Primer 3.0. Furthermore, a system for identifying clones of C. paliurus was constructed based on selected SSR markers with high reproducibility and stability.【Result】(1) We detected 89 741 SSR loci from the whole genome, with an occurrence frequency of 62.07%. (2) Among all SSR loci, the proportion of SSRs with a mononucleotide motif was the highest (62.67%) and a hexa-nucleotide repeat was the lowest (0.15%). Most of the repeated motifs in the SSR loci were dominated by (A/T)n. (3) The repeat number of mono-nucleotide and di-nucleotide motifs ranged from 6 to 16. With the increase in the repeat number, the frequencies of various SSR repetition types displayed a downward trend. (4) The length of the SSR sequences varied from 10 to 476 bp, and this length variation existed in different repetitive motifs. Additionally, the frequency of SSR occurrence tended to decrease as the repeat number increased. (5) We successfully designed 78 285 pairs of SSR primers using Primer 3.0. A total of 377 primer pairs were randomly synthesized for amplifying polymorphic SSR fragments, among which 75 pairs primers were successful. Moreover, quick response code DNA molecular ID cards for 19 medical-use clones of C. paliurus were constructed by five pairs of polymorphic SSR primers with a mono-nucleotide motif.【Conclusion】The frequency of genomic SSR loci was high, and there was variability in the type of SSR loci. Simple repeat sequences developed from the whole genome of C. paliurus could be effective candidate molecular markers with further applications in germplasm resource evaluation and fingerprint construction for multi-use clones of C. paliurus. Table and Figures | Reference | Related Articles | Metrics | Comments（0） Cited:

Select

A comparative analysis of sugar metabolism-related characteristics in different development stage fruits of six blueberry cultivars YANG Haiyan, WU Yaqiong, ZHANG Chunhong, LYU Lianfei, WU Wenlong, WEI Linxiao, LI Weilin Journal of Nanjing Forestry University (Natural Sciences Edition）    2025, 49 (3): 73-82.   DOI: 10.12302/j.issn.1000-2006.202410028 Abstract （1939）   HTML （1354）    PDF（pc） （3072KB）（1127）       Knowledge map    Save 【Objective】The purpose of this study is to understand the changes in sugar and other quality indexes during fruit development, to explore the potential mechanism of carbohydrate accumulation during blueberry (Vaccinium spp.) fruit development, and to establish a theoretical foundation for blueberry quality enhancement.【Method】Fruits of six blueberry cultivars at different developmental stages were used as the test materials. The morphological traits (size and color), antioxidant system-related parameters, fruit quality indexes, the expression levels of VcSWEETs genes, and sugar composition and content were determined. The correlation analysis among these parameters was performed using the Pearson correlation model.【Result】With the development of the fruit, the fruits of each cultivar gradually increased in size and deepened in color, of which the ‘Legacy’ cultivar had the largest fruit weight and transverse diameter, and ‘Zhongzhi 2’ had the smallest during the purple fruit stage (S3). In the S3 period, ‘Legacy’ showed peak L value with minimal a* and b* values. The generation rate of O_2^{\stackrel{-}{\underset{·}{ }}} and MDA content steadily increased, while H2O2 content (excluding ‘Zhongzhi 4’) and SOD activity decreased first and then increased, and POD activity gradually increased. Total soluble solids (TSS) and anthocyanin contents gradually increased, flavonoid content gradually decreased, and ellagic acid content initially decreased and then increased. Among them, ‘Zhongzhi 4’ had the highest TSS and anthocyanin content during the S3 period. The expression patterns of three VcSWEETs genes exhibited distinct cultivar specificity and developmental stage dependence. Sucrose, glucose, fructose, and total sugar accumulated continuously, though glucose maintained basal levels. In the S3 period, the total sugar contents of all cultivars were more than 200 mg/g, except for ‘Zhongzhi 1’.【Conclusion】Sugar content increased continually during fruit development, with sucrose and fructose concentrations predominating over glucose. ‘Legacy’ had the greatest total sugar content during the S3 period, followed by ‘Zhongzhi 2’ and ‘Zhongzhi 4’. The expansion of the fruit, the rise in anthocyanin content and POD activity, and the up-regulated expression of the VcSWEET1 gene were significantly correlated with sugar accumulation patterns. Table and Figures | Reference | Related Articles | Metrics | Comments（0） Cited:

Select

Morphological and physiological responses of male and female Ginkgo biloba to temperature changes WU Changfeng, GUO Jing, WANG Guibin Journal of Nanjing Forestry University (Natural Sciences Edition）    2024, 48 (4): 150-158.   DOI: 10.12302/j.issn.1000-2006.202209064 Abstract （2461）   HTML （523）    PDF（pc） （1958KB）（1115）       Knowledge map    Save 【Objective】This study investigated the response mechanism of ginkgo (Ginkgo biloba) to temperature changes and its gender differences, and clarified the relationship between changes in ginkgo morphology and physiological indexes and their corresponding temperature adaptation.【Method】We explored the response of male and female ginkgo trees to temperature changes under field conditions by measuring the growth index, anatomical structure, and metabolite content of adult ginkgo trees in five areas with an average annual precipitation of 990-1 200 mm and average annual temperatures of 9.8, 14.4, 16.3, 18.0 and 19.8 ℃, respectively.【Result】Under suitable living conditions, the leaf area, tree height, breast diameter, photosynthetic rate (Pn), and terpene lactone and polyvalerenol contents increased with the temperature, while the total flavonoids, polyphenols, polysaccharides, and soluble sugars contents decreased. These indicated that a certain degree of warming was beneficial to the growth and photosynthesis of Ginkgo biloba and increased the content of major metabolites such as terpene lactones and polypentenol, yet it was not promoting the accumulation of antioxidant metabolites. The tree height, leaf area, and total flavonoids, polyphenols, polysaccharides, and soluble sugar contents of male plants were marked higher than those of female plants, while the intercellular CO2 concentration (Ci) was lower than that of female plants.【Conclusion】Under natural conditions, both male and female ginkgo can adjust their morphological characteristics, photosynthetic strategies, and metabolite contents according to different temperature conditions to ensure the normal growth of individuals. The annual average temperature of 16.3-18.0 ℃ is the optimal environmental temperature for G. biloba growth. There exist gender differences in the adaptation of G. biloba to temperature, with the male plant exhibiting a superior ability to adapt to temperature changes. Table and Figures | Reference | Related Articles | Metrics | Comments（0） Cited:

Select

The influence of plant root splitting on garden rockeries in Jiangnan: taking the Zhanyuan Garden as an example ZHANG Qingping, CHEN Fengyi Journal of Nanjing Forestry University (Natural Sciences Edition）    2024, 48 (4): 271-278.   DOI: 10.12302/j.issn.1000-2006.202301025 Abstract （1761）   HTML （362）    PDF（pc） （2941KB）（1102）       Knowledge map    Save 【Objective】Jiangnan garden rockeries are a critical aspect of garden heritage. The widespread issue of plant root splitting poses a serious threat to the longevity and preservation of these rockeries. Incorporating the study of plant root splitting into the preventive protection system for rockeries can strengthen the system’s foundation, leading to a more effective preservation of garden heritage.【Method】This study uses the Zhanyuan Garden in Nanjing as a case study to quantitatively analyze the mechanism of plant root splitting in rockeries. It identifies problematic tree species, proposes and verifies five plant variables significantly related to root splitting, and establishes a regression prediction model for the area of cracks caused by this phenomenon.【Result】The study found that tree species with straight and deep roots are more likely to cause cracks in artificial rockeries. In the Zhanyuan Garden, the most significant root splitting was observed in Puja and privet, followed by loquat, Longjaya sophora, Magnolia, and papaya. Five factors,namely root length, root diameter, canopy width, tree height, and base diameter,were identified as highly influential in root splitting, showing significant correlations with the area of the resulting cracks. A multiple regression analysis helped to construct a preliminary prediction model for rockery crack areas, setting a warning threshold for changes in rockery crack dimensions. It was suggested that inhibiting the growth of a single root system might be necessary if the root length changes by more than 11.25 cm while the diameter remains constant.【Conclusion】Plant root splitting accelerates the development and expansion of cracks on rockery surfaces, potentially leading to stone fractures and structural collapse, thus endangering the heritage’s long-term preservation. Based on the quantitative analysis, refining environmental variables, categorizing monitoring levels, and defining clear warning indicators can enhance the predictive capabilities and quantitative assessments for rockery protection. These measures aim to facilitate timely interventions to mitigate the impact of root splitting and improve the overall preventive protection system for rockery heritage. Table and Figures | Reference | Related Articles | Metrics | Comments（0） Cited:

Select

Research on the ecological compensation mechanism and standards for Qianjiangyuan-Baishanzu National Park YAO Hongwen, WANG Shihong, ZHU Chenghao, LI Zuohui, CHENG Linghong, LIAN Jihe, LIN Jie Journal of Nanjing Forestry University (Natural Sciences Edition）    2024, 48 (5): 21-27.   DOI: 10.12302/j.issn.1000-2006.202309044 Abstract （2090）   HTML （493）    PDF（pc） （1433KB）（1094）       Knowledge map    Save 【Objective】The study aims to establish a sound ecological compensation standard, which will provide a sustainable and stable financial guarantee for the construction of national parks. 【Method】 Taking the Qianjiangyuan-Baishanzu National Park as an example, this study calculated the upper and lower range of the national park compensation standard by comparing the difference values of provincial public welfare forests inside and outside the national park with regulating services and opportunity cost losses. 【Result】 Considering the value of forest ecosystem regulation services and opportunity cost losses comprehensively, the value of provincial public welfare forests within the national park reached 47 237.01 yuan/(hm2·a).Among them, the value of provincial public welfare forests regulating service function was 45 672.53 yuan/(hm2·a), which was 13 306.25 yuan/(hm2·a) higher than outside the scope, and the value of each regulating service was higher than that outside the implementation area. The opportunity cost loss of public welfare forests within the national park was 1 564.48 yuan/(hm2·a), which still showed a gap of 841.48 yuan/(hm2·a) compared with the current highest compensation standard of 723 yuan/(hm2·a) of the provincial public welfare forests. 【Conclusion】 At present, there was still a large gap between the ecological compensation standard implemented in Qianjiangyuan-Baisanzu National Park and that calculated based on the regulating service unit area and opportunity cost. In the future, we suggest that the compensation standard be gradually increased year by year, taking into account the actual development needs of the National Park. Table and Figures | Reference | Supplementary Material | Related Articles | Metrics | Comments（0） Cited:

Select

New records of lianas in Hunan Province TIAN Weishuo, TU Ronghui, LIU Ang, DING Cong, YU Xunlin Journal of Nanjing Forestry University (Natural Sciences Edition）    2024, 48 (4): 46-48.   DOI: 10.12302/j.issn.1000-2006.202206016 Abstract （2697）   HTML （444）    PDF（pc） （2247KB）（1030）       Knowledge map    Save Five species (Isotrema hyperxanthum (X.X. Zhu & J.S. Ma) X.X. Zhu, et al., Dalbergia velutina Benth., Trichosanthes rubriflos Thorel ex Cayla, Albizia corniculata (Lour.) Druce, Capparis cantoniensis Lour.,) and one variety (Ventilago leiocarpa var. pubescens Y.L. Chen & P.K. Chou) of liana were reported as new records from Hunan Province, China. The voucher specimens were deposited in the Herbarium of the Central South University of Forestry and Technology, Hunan, China. Table and Figures | Reference | Related Articles | Metrics | Comments（0）

Select

Journal of Nanjing Forestry University (Natural Sciences Edition）    2024, 48 (5): 0-0.   Abstract （1062）      PDF（pc） （3276KB）（1029）       Knowledge map    Save Related Articles | Metrics | Comments（0）

Select

Effect of variations in fertilization formula on the photosynthetic characteristics and growth of young Sapindus saponaria LIU Juntao, JIA Liming, YAN Xiaoli, ZHANG Weihua, CAI Wanting, ZHONG Jing, WANG Lixian, CAO Qiuli, ZHAO Pengli, CHEN Yiyong, YU Jiaxin, CHEN Na, WENG Xuehuang Journal of Nanjing Forestry University (Natural Sciences Edition）    2024, 48 (6): 23-33.   DOI: 10.12302/j.issn.1000-2006.202303013 Abstract （1036）   HTML （282）    PDF（pc） （1752KB）（1020）       Knowledge map    Save 【Objective】The study aimed to explore the effects of the combined application of nitrogen (N), phosphorus (P), and potassium (K) fertilizers on the growth and photosynthetic characteristics of the ‘Yuanhua’ and ‘Yueshuo Bodhi’ of Sapindus saponaria, and to provide a theoretical basis for the scientific fertilization of disease-free clones.【Method】Using the S. saponaria ‘Yuanhua’ and ‘Yueshuo Bodhi’ as the experimental materials, the “3414” field experimental method was used to study the effects of different N, P, and K fertilization regimes on the growth and photosynthetic characteristics of the S. saponaria.【Result】The tree heights of the offspring of S. saponaria were greatest following treatment with N2P1K2 and N2P2K3, being 28.42% and 39.14% higher, respectively, than that of the control, and the differences were found to be significant (P < 0.05). The ground diameter was largest following treatment with N1P2K1 and N2P3K2, and increased by 16.11% and 32.93%, respectively, compared to that of the control (N0P0K0). The tree heights and ground diameters of the ‘Yuanhua’ were in the following order: P > N > K, under different fertilization treatments, while those of the ‘Yueshuo Bodhi’ were in the order: N > K > P. The net photosynthetic rate of the ‘Yuanhua’ and ‘Yueshuo Bodhi’ was highest following treatment with N2P2K2 under different fertilization regimes, and increased significantly by 42.19% and 53.01%, respectively, compared to that of the control. The net photosynthetic rate, intercellular CO2 concentration, rate of transpiration, and foliar water use efficiency of the treatment groups improved significantly; however, the stomatal conductance of the ‘Yuanhua’ did not alter significantly, and the stomatal conductance of the ‘Yueshuo Bodhi’ differed significantly, The quantity of N fertilizer applied exhibited a significant positive correlation with the tree heights and ground diameters of ‘Yuanhua’ and ‘Yueshuo Bodhi’ trees. The growth and photosynthetic indicators of the ‘Yuanhua’ and ‘Yueshuo Bodhi’ under different fertilization regimes were comprehensively evaluated using the entropy weighted TOPSIS method. The findings revealed that N2P1K2 and N1P2K2 were superior for the fertilization of ‘Yuanhua’ trees, while N1P1K2 and N2P2K2 were superior for the fertilization of the ‘Yueshuo Bodhi’.【Conclusion】The application of N, P and K at an appropriate ratio can effectively promote the growth and photosynthetic characteristics of ‘Yuanhua’ and ‘Yueshuo Bodhi’ trees. The optimal strategy for the application of N, P, and K fertilizers is that the quantities of N, P and K should be 115.26, 64.14 and 44.37 kg/hm2, respectively, for the ‘Yuanhua’, and 117.90, 87.65 and 36.11 kg/hm2, respectively, for ‘Yueshuo Bodhi’ trees. Table and Figures | Reference | Supplementary Material | Related Articles | Metrics | Comments（0）

Select

Comprehensive effect of understory medicinal herb cultivation on individual volume growth in near-mature, mature and overmature Cunninghamia lanceolata forests YE Limin, XU Yuanke, ZHOU Yizhi, CHEN Zhenglu, WANG Yixiang, GE Hongli Journal of Nanjing Forestry University (Natural Sciences Edition）    2024, 48 (5): 48-56.   DOI: 10.12302/j.issn.1000-2006.202312027 Abstract （1865）   HTML （403）    PDF（pc） （2936KB）（1017）       Knowledge map    Save 【Objective】 In the past, the understory planting was primarily conducted in young to middle-aged forests. However, with the advancement of public welfare forest development and the promotion of large-diameter timber cultivation, the issue of understory planting in mature stands has become an urgent research topic. This study aims to investigate the comprehensive effects of understory medicinal herb cultivation activities, including related shrubs cleaning up, land preparation, fertilization, routine care, and the growth of herbs, on the individual tree volume growth in mature Chinese fir (Cunninghamia lanceolata) forests. 【Method】 A total of 10 plots with understory planting of medicinal herbs and 10 plots without understory planting were established in mature Chinese fir plantations within Daji Forest Farm and Caoyu Pond Forest Farm, Jingning She Autonomous County, located in the southwestern part of Zhejiang Province. The medicinal herbs have been growing for a period of three years. One average tree from each plot was selected for wood analysis. The impact analysis of understory planting was conducted using a widely adaptable linear modeling approach, considering the annual growth measurements of volume, diameter, and height over the past three years. 【Result】 Under the simultaneous consideration of factors such as age, diameter at breast height, tree height, density, and site index, understory planting of medicinal herbs showed a significant promotional effect on individual tree volume growth. 【Conclusion】 With appropriate measures, understory planting can still significantly promote the volume growth of mature Chinese fir trees. However, this promotion is constrained by the age of the tree. The linear modeling approach is particularly suitable for analyzing data in situations where complexity exists and strict experimental control is not feasible. This method offers flexibility and provides reliable results. In small sample cases when using this method, it is necessary to perform a series of checks on the data to determine if the conditions are met in the course of modelling. Application of remedial measures to data that do not meet requirements. Table and Figures | Reference | Related Articles | Metrics | Comments（0） Cited:

Select

An improved CART model for leaf and wood classification from LiDAR point clouds of Quercus glauca individual trees PAN Zhengshang, MA Kaisen, LONG Yi, LAI Zhengui, SUN Hua Journal of Nanjing Forestry University (Natural Sciences Edition）    2024, 48 (4): 123-131.   DOI: 10.12302/j.issn.1000-2006.202211006 Abstract （2200）   HTML （356）    PDF（pc） （3088KB）（1005）       Knowledge map    Save 【Objective】Due to the complex structure and features, traditional classification models for tree branches and leaf point clouds typically face several problems, including poor stability, low accuracy, model overfitting, and high computational costs. In this study, we propose an improved CART (classification and regression tree) model for leaf and branch classification based on Quercus glauca individual tree point cloud data from terrestrial laser LiDAR.【Method】First, the feature descriptor was constructed according to the neighborhood points, and the optimal value of the neighborhood search parameter was then determined. The CART model was improved by gradually introducing variables and adjusting the structure of the decision tree. The classification results of the improved CART model were compared with those of the Logistics regression and K-nearest neighbor (KNN) models.【Result】The accuracy of the improved CART model using the test data increased after introducing the feature descriptors as variables, exceeding that of the Logistics regression and KNN model by 13.1% and 13.6%, respectively. Moreover, the improved CART model exhibited higher accuracy, better stability, and marked reduced model size following the improvement. In particular, the model size was reduced by 99.9% compared with before the improvement, while the data training time was only 51.3% of that before the adjustment. The comprehensive evaluation index of the improved CART model was approximately 0.9 on both trunk and leaf data, with the difference between accuracy on train data and test data lower than 0.001, indicating no overfitting.【Conclusion】The improved CART model has a high accuracy and stability, and achieves good classification results on small samples. This study provides a methodological reference for the accurate and rapid classification of trunk and leaf point clouds from terrestrial laser LiDAR. Table and Figures | Reference | Supplementary Material | Related Articles | Metrics | Comments（0） Cited:

Select

An prediction of the potential distribution of suitable habitat for Grus leucogeranus using the MaxEnt model LIU Xiaoyan, ZHANG Zengxin, LI Jun, CHEN Juan, HUA Jun, PENG Ye, YAN Xin, QIU Jian Journal of Nanjing Forestry University (Natural Sciences Edition）    2024, 48 (5): 181-188.   DOI: 10.12302/j.issn.1000-2006.202303053 Abstract （2432）   HTML （352）    PDF（pc） （3409KB）（1001）       Knowledge map    Save 【Objective】The middle and lower reaches of the Yangtze River basin are the most important wintering habitats for the Grus leucogeranus. However, in recent years, the distribution of suitable habitats for the G. leucogeranus in this area has been greatly altered by the combined effects of climate change and various other factors. Therefore, conducting research on the habitat suitability of G. leucogeranus in this area is of great significance for the protection and scientific management of this endangered species.【Method】In this study, based on the changes in the distribution of suitable areas of the G. leucogeranus wintering habitat in China, we used the Maximum Entropy Model (MaxEnt) to predict the potential suitable areas of the G. leucogeranus wintering habitat. The prediction was based on the G. leucogeranus distribution points, vegetation, topography, observed air temperature, precipitation, Global Climate Models (GCMs) data, and other various environmental factors.【Result】(1) The MaxEnt model proved highly effective in predicting the distribution of suitable habitats and assessing climate suitability for G. leucogeranus in their wintering grounds, with an AUC value of 0.978. The most influential environmental factors affecting the distribution of suitable habitat for G. leucogeranus were elevation, precipitation during the wettest month, slope, and NDVI. (2) The contemporary suitable habitat of the G. leucogeranus mainly distributed in the middle and lower reaches of the Yangtze River, with the the Poyang Lake being the most important high suitable area for the species. The medium and low suitable areas also mainly distributed around the the Poyang Lake. In addition, low suitable areas were found in the middle and lower reaches of the Yangtze River, along the coast of the Bohai Bay, and in the Haihe River, the Liaohe River, the Nenjiang, and the Songhua River basins. (3) Global climate model prediction results showed predictions indicate that the next 20 years, although the low suitable areas for G. leucogeranus during wintering is on the rise, the medium and high suitable areas generally decreasing. Among them, compared to the contemporary period, the medium suitable living area are projected to shrink by 2 500 to 25 700 km2, and the high suitable living area will shrink by 3 800 to 12 200 km2.【Conclusion】Different greenhouse gas emission scenarios will have different varying impacts on the distribution of G. leucogeranus. In the context of global warming, the wintering habitat of G. leucogeranus may shrink significantly, which will pose a serious challenge to the conservation of this rare species, and we should strengthen the research on G. leucogeranus habitat in the context of climate change, to provide data support for the conservation of G. leucogeranus and the management of their habitats. Table and Figures | Reference | Related Articles | Metrics | Comments（0） Cited:

Select

A comparative study on leaf characters between diploid and tetraploid of Cyclocarya paliurus LIU Xialan, SONG Ziqi, HU Fengrong, SHANG Xulan Journal of Nanjing Forestry University (Natural Sciences Edition）    2024, 48 (4): 76-84.   DOI: 10.12302/j.issn.1000-2006.202305024 Abstract （3226）   HTML （1183）    PDF（pc） （2639KB）（997）       Knowledge map    Save 【Objective】To compare the differences in leaf phenotypes characters, anatomical structures and stomatal characteristics between diploid and tetraploid Cyclocarya paliurus, we obtained data on the leaf characteristics of C. paliurus with different ploidy.【Method】Diploid and tetraploid seedlings of C. paliurus were used as study materials, and 32 leaf traits were measured. Independent samples t-tests, a correlation analysis, and principal component analysis (PCA) were conducted.【Result】There were no significant differences between diploid and tetraploid seedlings for sawtooth width and the position of the maximum leaf width of the middle leaflet. For the other 30 leaf traits, there were significant or extremely significant differences between diploid and tetraploid seedlings. Among them, the number of leaflets of tetraploids was significantly less than that of diploids, while the area and specific leaf weight of compound leaves of tetraploids were significantly greater than those of diploids. The length, width and area of the terminal and middle leaflets of tetraploids were significantly greater than those of diploids, while the leaf shape index was significantly smaller than that of diploids. The sawtooth density of tetraploids was significantly smaller than that of diploids. The leaflet thickness, the upper epidermis thickness, lower epidermis thickness, palisade tissue thickness, and sponge tissue thickness of tetraploids were all significantly higher than those of diploids, while the ratio of palisade tissue to sponge tissue and cell tense ratio were significantly smaller than those of diploids. The stomatal length and width of tetraploids were significantly greater than those of diploids, while the stomatal density was significantly smaller than that of diploids. The correlation analysis results indicated that there were broad and significant correlations between the phenotypic characteristics, anatomical structure, and stomatal characteristics of C. paliurus. The indicators reflecting the differences of leaf characteristics between diploid and tetraploid C. paliurus were extracted by a PCA, including leaflet width, leaflet area, leaf shape index, sponge tissue thickness, stomatal length, palisade tissue thickness and cell tense ratio.【Conclusion】There were significant differences in leaf traits between diploid and tetraploid C. paliurus, and leaf phenotypic characteristics, such as leaflet width, leaflet area, and leaf shape index, can be used for the preliminary screening of diploid and tetraploid C. paliurus. Table and Figures | Reference | Supplementary Material | Related Articles | Metrics | Comments（0） Cited:

Select

The growth and photosynthetic characteristics of Acer truncatum seedlings in response to soil water and fertilizer coupling LI Panting, DU Manyi, WANG Yue, PEI Shunxiang, XIN Xuebing Journal of Nanjing Forestry University (Natural Sciences Edition）    2024, 48 (5): 113-122.   DOI: 10.12302/j.issn.1000-2006.202302008 Abstract （2218）   HTML （318）    PDF（pc） （1794KB）（993）       Knowledge map    Save 【Objective】 This research aims to investigate the growth and photosynthetic physiological characteristics of Acer truncatum seedlings in response to water-fertilizer coupling, and to analyze the optimal water-fertilizer combination for the growth of A. truncatum seedlings. 【Method】 One-year-old A. truncatum seedlings were subjected to varying water and fertilizer couplings in an orthogonal test design involving four factors and four levels of soil water content, nitrogen, phosphorus and potassium, resulting in 16 treatments. Growth indexes, relative chlorophyll content of the leaves SPAD values, photosynthetic characteristics, and light response curves were measured after the treatments. 【Result】 The growth indices generally increased firstly and then decreased with increasing fertilization amount. At a soil water content of 75%,1.2, 1.8 and 0 g/plant of N, P, K, respectively, the diameter and total biomass of the plant were significantly higher than those of other treatments. Suitable soil water and fertilizer coupling could significantly improve the height, ground diameter, and total biomass of A. truncatum seedlings. Moreover, there was no significant difference in the utilization ability of low light among different soil water and fertilizer coupling treatments, and nitrogen fertilizer had significant effects on the maximum net photosynthetic rate and light saturation point of A. truncatum seedlings. In addition, there was no significant difference in water use efficiency (WUE) between the different treatments for A. truncatum seedlings. The seedlings grew normally even under lower water conditions, such as 45% soil water content, and exhibited strong drought resistance. The effects of soil water and fertilizer on the growth of A. truncatum seedlings were ranked from high to low as nitrogen fertilizer > soil water content > potash fertilizer > phosphate fertilizer, based on a comprehensive scoring method. The optimal combination of water and fertilizer was 75% soil water content, with fertilizer comprising 1.2 g of nitrogen, 1.8 g of phosphorous, and 0 g of potassium per plant. 【Conclusion】 Water-fertilizer coupling had a significant effect on ground diameter and biomass of A. truncatum seedlings, but an insignificant effect on seedling height, while soil water content and nitrogen fertilizer also had a significant effect on the growth indexes of the seedlings. These findings provide a theoretical basis for the appropriate use of soil water and fertilizer in A. truncatum. Table and Figures | Reference | Related Articles | Metrics | Comments（0） Cited:

Select

Effects of arbuscular mycorrhizal fungi on the growth and physiological characteristics of Xanthoceras sorbifolium under salt stress ZONG Jianwei, LI Cheng, ZHANG Jing, YANG Yuhua Journal of Nanjing Forestry University (Natural Sciences Edition）    2024, 48 (4): 168-176.   DOI: 10.12302/j.issn.1000-2006.202207008 Abstract （2077）   HTML （362）    PDF（pc） （1486KB）（991）       Knowledge map    Save 【Objective】An investigation on the effects of arbuscular mycorrhizal (AM) fungi inoculation on the growth, physiological metabolism, and salt tolerance of Xanthoceras sorbifolium under salt stress was conducted.【Method】In this study, Funneliformis mosseae was used to infect seedlings and the pot experiment was carried out using one-year-old X. sorbifolium seedlings. We applied different concentrations of NaCl stress (0, 80, 160, 240, and 320 mmol/L). After the stress period, the biomass, colonization characteristics, and physiological indicators of the X. sorbifolium seedlings were measured.【Result】(1)The aboveground and underground biomasses of X. sorbifolium seedlings were enhanced significantly by AM symbiosis upon exposure to salinity. Increasing salinity significantly caused a decrease in the mycorrhizal colonization rate.(2)AM fungi inoculation significantly improved the contents of soluble protein, proline, glutathione(GSH), and ascorbic acid (AsA), and resulted in an increasement in the activities of superoxide dismutase (SOD) and peroxidase (POD), yet a reduction in the relative conductivity and accumulation of malondialdehyde (MDA).(3)Furthermore, two-way analysis of variance(Two-way ANOVA)demonstrated that the duration and concentration were interactively and significantly affecting correlated with the content of MDA, proline, soluble protein, and AsA (P<0.01). Based on principal component analysis(PCA), it was found that the inoculation group had the most profound impact on the physiological characteristics of X. sorbifolium at 320 mmol/L NaCl treatment.【Conclusion】This study found that inoculation with AM fungi may advance osmotic mediation ability, significantly improve the activities of antioxidant enzymes and the contents of antioxidants, and enhance the salt tolerance of plants and eventually promote the growth of X. sorbifolium seedlings under different salt concentrations. Table and Figures | Reference | Supplementary Material | Related Articles | Metrics | Comments（0） Cited:

Select

Leaf phenotypic diversity analysis of holly germplasm resources WANG Xuejie, ZHOU Peng, HOU Sixuan, FANG Yanming, ZHANG Min Journal of Nanjing Forestry University (Natural Sciences Edition）    2024, 48 (5): 90-96.   DOI: 10.12302/j.issn.1000-2006.202302015 Abstract （2060）   HTML （363）    PDF（pc） （1949KB）（987）       Knowledge map    Save 【Objective】 This study aims to understand the leaf phenotypic diversity of holly (Ilex spp.) germplasm resources, elucidate the mechanisms behind this diversity, and support the introduction, cultivation and breeding of new varieties. 【Method】 We observed 18 phenotypic traits across 42 holly germplasm resources and analyzed the phenotypic variation using differential analysis, variance analysis, principal component analysis (PCA), and cluster analysis. 【Result】 There was significant variation in the leaf phenotypic traits among the holly germplasm resources, particularly in leaf area. The Shannon diversity index ranged from 1.00 to 2.03, indicating rich phenotypic diversity primarily due to interspecies variation. Correlation analysis showed a significant negative relationship between external leaf traits and anatomical traits. PCA identified four principal components, namely leaf length, tissue compactness, palisade tissue thickness, and stomatal density, that accounted for 85.20% of the variation, effectively capturing the overall characteristics of the holly plants. Cluster analysis grouped the 42 resources into four categories based on their leaf traits: large leaf with large petiole, small leaf with small petiole, medium leaf with hard serrations, and medium leaf with thin texture. 【Conclusion】 The study confirms substantial intrageneric phenotypic diversity in holly, driven predominantly by interspecies differences. External leaf traits were pivotal in phenotypic differentiation. The identified principal components, namely leaf length, compactness, palisade structure, and stomatal density, are crucial for classifying and identifying holly germplasm resources. Based on the leaf phenotypes, the resources were categorized into four distinct groups, providing a theoretical foundation for further classification, resource utilization, and breeding within the genus Ilex L. Table and Figures | Reference | Related Articles | Metrics | Comments（0）